5k28 - Revision history

OCA at 19:18, 20 September 2023

2023-09-20T19:18:53Z

←Older revision		Revision as of 19:18, 20 September 2023
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	==Structure of the unbound SH3 domain of MLK3==		==Structure of the unbound SH3 domain of MLK3==
-	<StructureSection load='5k28' size='340' side='right' caption='[[5k28]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='5k28' size='340' side='right'caption='[[5k28]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k28]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K28 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5K28 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k28]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K28 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5k26\|5k26]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">MAP3K11, MLK3, PTK1, SPRK ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=~~5&~~id=9606 HUMAN])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k28 OCA], [https://pdbe.org/5k28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k28 RCSB], [https://www.ebi.ac.uk/pdbsum/5k28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k28 ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mitogen-activated_protein_kinase_kinase_kinase Mitogen-activated protein kinase kinase kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.25 2.7.11.25] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5k28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k28 OCA], [~~http~~://pdbe.org/5k28 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5k28 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5k28 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5k28 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/M3K11_HUMAN M3K11_HUMAN]] Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.<ref>PMID:12529434</ref> <ref>PMID:15258589</ref> <ref>PMID:8195146</ref> <ref>PMID:9003778</ref>	+	[https://www.uniprot.org/uniprot/M3K11_HUMAN M3K11_HUMAN] Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.<ref>PMID:12529434</ref> <ref>PMID:15258589</ref> <ref>PMID:8195146</ref> <ref>PMID:9003778</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Mitogen-activated protein kinase kinase kinase~~]]	+	[[Category: Large Structures]]
-	[[Category: Kall~~, S K~~]]	+	[[Category: Kall SK]]
-	[[Category: Lavie, A]]	+	[[Category: Lavie A]]
-	~~[[Category: Mlk3]]~~	+
-	~~[[Category: Phage display]]~~	+
-	~~[[Category: Sh3]]~~	+
-	~~[[Category: Signalling protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:28, 26 December 2018

2018-12-26T08:28:42Z

←Older revision		Revision as of 08:28, 26 December 2018
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	<StructureSection load='5k28' size='340' side='right' caption='[[5k28]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='5k28' size='340' side='right' caption='[[5k28]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k28]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K28 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k28]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K28 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k26\|5k26]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k26\|5k26]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAP3K11, MLK3, PTK1, SPRK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mitogen-activated_protein_kinase_kinase_kinase Mitogen-activated protein kinase kinase kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.25 2.7.11.25] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mitogen-activated_protein_kinase_kinase_kinase Mitogen-activated protein kinase kinase kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.25 2.7.11.25] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k28 OCA], [http://pdbe.org/5k28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k28 RCSB], [http://www.ebi.ac.uk/pdbsum/5k28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k28 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k28 OCA], [http://pdbe.org/5k28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k28 RCSB], [http://www.ebi.ac.uk/pdbsum/5k28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k28 ProSAT]</span></td></tr>
Line 10:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/M3K11_HUMAN M3K11_HUMAN]] Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.<ref>PMID:12529434</ref> <ref>PMID:15258589</ref> <ref>PMID:8195146</ref> <ref>PMID:9003778</ref>		[[http://www.uniprot.org/uniprot/M3K11_HUMAN M3K11_HUMAN]] Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.<ref>PMID:12529434</ref> <ref>PMID:15258589</ref> <ref>PMID:8195146</ref> <ref>PMID:9003778</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mixed-lineage kinase 3 (MLK3; also known as MAP3K11) is a Ser/Thr protein kinase widely expressed in normal and cancerous tissues, including brain, lung, liver, heart, and skeletal muscle tissues. Its Src homology 3 (SH3) domain has been implicated in MLK3 autoinhibition and interactions with other proteins, including those from viruses. The MLK3 SH3 domain contains a six-amino-acid insert corresponding to the n-Src insert, suggesting that MLK3 may bind additional peptides. Here, affinity selection of a phage-displayed combinatorial peptide library for MLK3's SH3 domain yielded a 13-mer peptide, designated "MLK3 SH3-interacting peptide" (MIP). Unlike most SH3 domain peptide ligands, MIP contained a single proline. The 1.2-A crystal structure of the MIP-bound SH3 domain revealed that the peptide adopts a beta-hairpin shape, and comparison with a 1.5-A apo SH3 domain structure disclosed that the n-Src loop in SH3 undergoes an MIP-induced conformational change. A 1.5-A structure of the MLK3 SH3 domain bound to a canonical proline-rich peptide from hepatitis C virus nonstructural 5A (NS5A) protein revealed that it and MIP bind the SH3 domain at two distinct sites, but biophysical analyses suggested that the two peptides compete with each other for SH3 binding. Moreover, SH3 domains of MLK1 and MLK4, but not MLK2, also bound MIP, suggesting that the MLK1-4 family may be differentially regulated through their SH3 domains. In summary, we have identified two distinct peptide-binding sites in the SH3 domain of MLK3, providing critical insights into mechanisms of ligand binding by the MLK family of kinases.
		+
		+	Identification of two distinct peptide-binding pockets in the SH3 domain of human mixed-lineage kinase 3.,Kokoszka ME, Kall SL, Khosla S, McGinnis JE, Lavie A, Kay BK J Biol Chem. 2018 Aug 31;293(35):13553-13565. doi: 10.1074/jbc.RA117.000262. Epub, 2018 Jul 6. PMID:29980598<ref>PMID:29980598</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5k28" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Mitogen-activated protein kinase kinase kinase]]		[[Category: Mitogen-activated protein kinase kinase kinase]]
	[[Category: Kall, S K]]		[[Category: Kall, S K]]

OCA at 06:53, 13 December 2017

2017-12-13T06:53:25Z

←Older revision		Revision as of 06:53, 13 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5k28 is ~~ON HOLD until Dec 13 2018~~	+	==Structure of the unbound SH3 domain of MLK3==
-		+	<StructureSection load='5k28' size='340' side='right' caption='[[5k28]], [[Resolution\|resolution]] 1.50Å' scene=''>
-	~~Authors~~: ~~Kall~~, S.K., ~~Lavie~~, A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5k28]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K28 FirstGlance]. <br>
-	~~Description~~: ~~Structure of~~ the ~~unbound SH3 domain~~ of ~~MLK3~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5k26\|5k26]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mitogen-activated_protein_kinase_kinase_kinase Mitogen-activated protein kinase kinase kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.25 2.7.11.25] </span></td></tr>
-	[[Category: Kall, S.K]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k28 OCA], [http://pdbe.org/5k28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k28 RCSB], [http://www.ebi.ac.uk/pdbsum/5k28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k28 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/M3K11_HUMAN M3K11_HUMAN]] Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.<ref>PMID:12529434</ref> <ref>PMID:15258589</ref> <ref>PMID:8195146</ref> <ref>PMID:9003778</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Mitogen-activated protein kinase kinase kinase]]
		+	[[Category: Kall, S K]]
	[[Category: Lavie, A]]		[[Category: Lavie, A]]
		+	[[Category: Mlk3]]
		+	[[Category: Phage display]]
		+	[[Category: Sh3]]
		+	[[Category: Signalling protein]]
		+	[[Category: Transferase]]

OCA at 06:21, 8 November 2017

2017-11-08T06:21:12Z

←Older revision		Revision as of 06:21, 8 November 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5k28 is ON HOLD until ~~Nov 22~~ 2018	+	The entry 5k28 is ON HOLD until Dec 13 2018

	Authors: Kall, S.K., Lavie, A.		Authors: Kall, S.K., Lavie, A.

OCA at 10:06, 17 May 2017

2017-05-17T10:06:31Z

←Older revision		Revision as of 10:06, 17 May 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5k28 is ON HOLD until ~~Paper Publication~~	+	The entry 5k28 is ON HOLD until Nov 22 2018

	Authors: Kall, S.K., Lavie, A.		Authors: Kall, S.K., Lavie, A.

OCA at 23:56, 12 July 2016

2016-07-12T23:56:16Z

←Older revision		Revision as of 23:56, 12 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5k28 is ON HOLD	+	The entry 5k28 is ON HOLD until Paper Publication

	Authors: Kall, S.K., Lavie, A.		Authors: Kall, S.K., Lavie, A.

OCA: Protected "5k28" [edit=sysop:move=sysop]

2016-06-01T14:13:05Z

Protected "5k28" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:13, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5k28 is ON HOLD Authors: Kall, S.K., Lavie, A. Description: Structure of the unbound SH3 domain of MLK3 Category: Unreleased Structures [[Categ...

2016-06-01T14:13:04Z

New page: '''Unreleased structure''' The entry 5k28 is ON HOLD Authors: Kall, S.K., Lavie, A. Description: Structure of the unbound SH3 domain of MLK3 Category: Unreleased Structures [[Categ...

New page

'''Unreleased structure'''

The entry 5k28 is ON HOLD

Authors: Kall, S.K., Lavie, A.

Description: Structure of the unbound SH3 domain of MLK3
[[Category: Unreleased Structures]]
[[Category: Kall, S.K]]
[[Category: Lavie, A]]