5k3w - Revision history

OCA at 10:39, 27 September 2023

2023-09-27T10:39:12Z

←Older revision		Revision as of 10:39, 27 September 2023
Line 3:		Line 3:
	<StructureSection load='5k3w' size='340' side='right'caption='[[5k3w]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='5k3w' size='340' side='right'caption='[[5k3w]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k3w]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_19096 Atcc 19096~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K3W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5K3W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k3w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Curtobacterium_pusillum Curtobacterium pusillum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K3W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K3W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAB:3-AMINOBENZOIC+ACID'>GAB</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.503Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5k3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k3w OCA], [~~http~~://pdbe.org/5k3w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5k3w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5k3w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5k3w ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAB:3-AMINOBENZOIC+ACID'>GAB</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k3w OCA], [https://pdbe.org/5k3w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k3w RCSB], [https://www.ebi.ac.uk/pdbsum/5k3w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k3w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1S4NYF0_9MICO A0A1S4NYF0_9MICO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 19096]]
-	[[Category: Large Structures]]
-	[[Category: Diepold, M]]
-	[[Category: Gruber, K]]
-	[[Category: Pavkov-Keller, T]]
-	[[Category: Steiner, K]]
	[[Category: Curtobacterium pusillum]]		[[Category: Curtobacterium pusillum]]
-	[[Category: ~~Transaminase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Transferase~~]]	+	[[Category: Diepold M]]
		+	[[Category: Gruber K]]
		+	[[Category: Pavkov-Keller T]]
		+	[[Category: Steiner K]]

OCA at 09:17, 25 June 2020

2020-06-25T09:17:33Z

←Older revision		Revision as of 09:17, 25 June 2020
Line 1:		Line 1:

	==Structural characterisation of fold IV-transaminase, CpuTA1, from Curtobacterium pusillum==		==Structural characterisation of fold IV-transaminase, CpuTA1, from Curtobacterium pusillum==
-	<StructureSection load='5k3w' size='340' side='right' caption='[[5k3w]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='5k3w' size='340' side='right'caption='[[5k3w]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k3w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K3W OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5K3W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k3w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19096 Atcc 19096]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K3W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5K3W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAB:3-AMINOBENZOIC+ACID'>GAB</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAB:3-AMINOBENZOIC+ACID'>GAB</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5k3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k3w OCA], [http://pdbe.org/5k3w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k3w RCSB], [http://www.ebi.ac.uk/pdbsum/5k3w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k3w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5k3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k3w OCA], [http://pdbe.org/5k3w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k3w RCSB], [http://www.ebi.ac.uk/pdbsum/5k3w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k3w ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 19096]]
		+	[[Category: Large Structures]]
	[[Category: Diepold, M]]		[[Category: Diepold, M]]
	[[Category: Gruber, K]]		[[Category: Gruber, K]]

OCA at 19:02, 15 December 2016

2016-12-15T19:02:16Z

←Older revision		Revision as of 19:02, 15 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5k3w is ~~ON HOLD~~	+	==Structural characterisation of fold IV-transaminase, CpuTA1, from Curtobacterium pusillum==
		+	<StructureSection load='5k3w' size='340' side='right' caption='[[5k3w]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5k3w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K3W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K3W FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GAB:3-AMINOBENZOIC+ACID'>GAB</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k3w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k3w OCA], [http://pdbe.org/5k3w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k3w RCSB], [http://www.ebi.ac.uk/pdbsum/5k3w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k3w ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Transaminases are useful biocatalysts for the production of amino acids and chiral amines as intermediates for a broad range of drugs and fine chemicals. Here, we describe the discovery and characterisation of new transaminases from microorganisms which were enriched in selective media containing (R)-amines as sole nitrogen source. While most of the candidate proteins were clearly assigned to known subgroups of the fold IV family of PLP-dependent enzymes by sequence analysis and characterisation of their substrate specificity, some of them did not fit to any of these groups. The structure of one of these enzymes from Curtobacterium pusillum, which can convert d-amino acids and various (R)-amines with high enantioselectivity, was solved at a resolution of 2.4 A. It shows significant differences especially in the active site compared to other transaminases of the fold IV family and thus indicates the existence of a new subgroup within this family. Although the discovered transaminases were not able to convert ketones in a reasonable time frame, overall, the enrichment-based approach was successful, as we identified two amine transaminases, which convert (R)-amines with high enantioselectivity, and can be used for a kinetic resolution of 1-phenylethylamine and analogues to obtain the (S)-amines with e.e.s >99%.

-	~~Authors~~:	+	Discovery and structural characterisation of new fold type IV-transaminases exemplify the diversity of this enzyme fold.,Pavkov-Keller T, Strohmeier GA, Diepold M, Peeters W, Smeets N, Schurmann M, Gruber K, Schwab H, Steiner K Sci Rep. 2016 Dec 1;6:38183. doi: 10.1038/srep38183. PMID:27905516<ref>PMID:27905516</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5k3w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Diepold, M]]
		+	[[Category: Gruber, K]]
		+	[[Category: Pavkov-Keller, T]]
		+	[[Category: Steiner, K]]
		+	[[Category: Curtobacterium pusillum]]
		+	[[Category: Transaminase]]
		+	[[Category: Transferase]]

OCA: Protected "5k3w" [edit=sysop:move=sysop]

2016-06-01T14:17:27Z

Protected "5k3w" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:17, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5k3w is ON HOLD Authors: Description: Category: Unreleased Structures

2016-06-01T14:17:25Z

New page: '''Unreleased structure''' The entry 5k3w is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5k3w is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]