5k67 - Revision history

OCA at 10:41, 27 September 2023

2023-09-27T10:41:39Z

←Older revision		Revision as of 10:41, 27 September 2023
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	<StructureSection load='5k67' size='340' side='right'caption='[[5k67]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5k67' size='340' side='right'caption='[[5k67]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k67]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K67 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5K67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k67]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K67 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K67 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SI4:[CUII(BIOT-PR-DPEA)]2+'>SI4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5k67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k67 OCA], [~~http~~://pdbe.org/5k67 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5k67 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5k67 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5k67 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SI4:[CUII(BIOT-PR-DPEA)]2+'>SI4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k67 OCA], [https://pdbe.org/5k67 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k67 RCSB], [https://www.ebi.ac.uk/pdbsum/5k67 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k67 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Borovik, A S~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: ~~Heinisch, T~~]]	+	[[Category: Borovik AS]]
-	[[Category: ~~Hendrich, M R]]~~	+	[[Category: Heinisch T]]
-	~~[[Category: Mann, S I]]~~	+	[[Category: Hendrich MR]]
-	~~[[Category: Ward,~~ T R]]	+	[[Category: Mann SI]]
-	[[Category: ~~Weitz, A C~~]]	+	[[Category: Ward TR]]
-	[[Category: ~~Artificial metalloprotein~~]]	+	[[Category: Weitz AC]]
-	[[Category: ~~Beta-barrel~~]]	+
-	[[Category: ~~Biotin binding protein]]~~	+
-	~~[[Category: Copper protein]]~~	+
-	~~[[Category: Cupredoxin]]~~	+
-	~~[[Category: Tetramer~~]]	+

OCA at 09:22, 25 June 2020

2020-06-25T09:22:29Z

←Older revision		Revision as of 09:22, 25 June 2020
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	==Designed Artificial Cupredoxins==		==Designed Artificial Cupredoxins==
-	<StructureSection load='5k67' size='340' side='right' caption='[[5k67]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5k67' size='340' side='right'caption='[[5k67]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K67 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5K67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K67 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5K67 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SI4:[CUII(BIOT-PR-DPEA)]2+'>SI4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SI4:[CUII(BIOT-PR-DPEA)]2+'>SI4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5k67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k67 OCA], [http://pdbe.org/5k67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k67 RCSB], [http://www.ebi.ac.uk/pdbsum/5k67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k67 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5k67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k67 OCA], [http://pdbe.org/5k67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k67 RCSB], [http://www.ebi.ac.uk/pdbsum/5k67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k67 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5k67" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5k67" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Borovik, A S]]		[[Category: Borovik, A S]]
	[[Category: Heinisch, T]]		[[Category: Heinisch, T]]

OCA at 06:54, 31 January 2018

2018-01-31T06:54:55Z

←Older revision		Revision as of 06:54, 31 January 2018
Line 3:		Line 3:
	<StructureSection load='5k67' size='340' side='right' caption='[[5k67]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5k67' size='340' side='right' caption='[[5k67]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5k67]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K67 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5k67]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K67 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SI4:[CUII(BIOT-PR-DPEA)]2+'>SI4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SI4:[CUII(BIOT-PR-DPEA)]2+'>SI4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k67 OCA], [http://pdbe.org/5k67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k67 RCSB], [http://www.ebi.ac.uk/pdbsum/5k67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k67 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k67 OCA], [http://pdbe.org/5k67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k67 RCSB], [http://www.ebi.ac.uk/pdbsum/5k67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k67 ProSAT]</span></td></tr>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1583]]
	[[Category: Borovik, A S]]		[[Category: Borovik, A S]]
	[[Category: Heinisch, T]]		[[Category: Heinisch, T]]

OCA at 15:11, 26 July 2016

2016-07-26T15:11:18Z

←Older revision		Revision as of 15:11, 26 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5k67 is ~~ON HOLD until Paper~~ Publication	+	==Designed Artificial Cupredoxins==
		+	<StructureSection load='5k67' size='340' side='right' caption='[[5k67]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5k67]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5K67 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SI4:[CUII(BIOT-PR-DPEA)]2+'>SI4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5k67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k67 OCA], [http://pdbe.org/5k67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5k67 RCSB], [http://www.ebi.ac.uk/pdbsum/5k67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5k67 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cupredoxins are electron-transfer proteins that have active sites containing a mononuclear Cu center with an unusual trigonal monopyramidal structure (Type 1 Cu). A single Cu-Scys bond is present within the trigonal plane that is responsible for its unique physical properties. We demonstrate that a cysteine-containing variant of streptavidin (Sav) can serve as a protein host to model the structure and properties of Type 1 Cu sites. A series of artificial Cu proteins are described that rely on Sav and a series of biotinylated synthetic Cu complexes. Optical and EPR measurements highlight the presence of a Cu-Scys bond, and XRD analysis provides structural evidence. We further provide evidence that changes in the linker between the biotin and Cu complex within the synthetic constructs allows for small changes in the placement of Cu centers within Sav that have dramatic effects on the structural and physical properties of the resulting artificial metalloproteins. These findings highlight the utility of the biotin-Sav technology as an approach for simulating active sites of metalloproteins.

-	~~Authors:~~ Mann~~, S.I.~~, Heinisch, T., Weitz~~, A.C.~~, Hendrich~~, M.R.~~, Ward~~, T.R.~~, Borovik~~, A~~.S.	+	Modular Artificial Cupredoxins.,Mann SI, Heinisch T, Weitz AC, Hendrich MP, Ward TR, Borovik AS J Am Chem Soc. 2016 Jul 14. PMID:27385206<ref>PMID:27385206</ref>

-	~~Description: Designed Artificial Cupredoxins~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category: Weitz~~, A.~~C]]~~	+	<div class="pdbe-citations 5k67" style="background-color:#fffaf0;"></div>
-	~~[[Category~~: ~~Hendrich, M.R]]~~	+	== References ==
-	[[Category: Borovik, A.S]]	+	<references/>
-	~~[[Category: Mann, S.I]]~~	+	__TOC__
-	~~[[Category: Ward, T.R~~]]	+	</StructureSection>
		+	[[Category: Borovik, A S]]
	[[Category: Heinisch, T]]		[[Category: Heinisch, T]]
		+	[[Category: Hendrich, M R]]
		+	[[Category: Mann, S I]]
		+	[[Category: Ward, T R]]
		+	[[Category: Weitz, A C]]
		+	[[Category: Artificial metalloprotein]]
		+	[[Category: Beta-barrel]]
		+	[[Category: Biotin binding protein]]
		+	[[Category: Copper protein]]
		+	[[Category: Cupredoxin]]
		+	[[Category: Tetramer]]

OCA at 13:28, 20 June 2016

2016-06-20T13:28:17Z

←Older revision		Revision as of 13:28, 20 June 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5k67 is ON HOLD	+	The entry 5k67 is ON HOLD until Paper Publication

	Authors: Mann, S.I., Heinisch, T., Weitz, A.C., Hendrich, M.R., Ward, T.R., Borovik, A.S.		Authors: Mann, S.I., Heinisch, T., Weitz, A.C., Hendrich, M.R., Ward, T.R., Borovik, A.S.

OCA: Protected "5k67" [edit=sysop:move=sysop]

2016-06-01T14:22:47Z

Protected "5k67" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:22, 1 June 2016

OCA: New page: '''Unreleased structure''' The entry 5k67 is ON HOLD Authors: Mann, S.I., Heinisch, T., Weitz, A.C., Hendrich, M.R., Ward, T.R., Borovik, A.S. Description: Designed Artificial Cupredox...

2016-06-01T14:22:46Z

New page: '''Unreleased structure''' The entry 5k67 is ON HOLD Authors: Mann, S.I., Heinisch, T., Weitz, A.C., Hendrich, M.R., Ward, T.R., Borovik, A.S. Description: Designed Artificial Cupredox...

New page

'''Unreleased structure'''

The entry 5k67 is ON HOLD

Authors: Mann, S.I., Heinisch, T., Weitz, A.C., Hendrich, M.R., Ward, T.R., Borovik, A.S.

Description: Designed Artificial Cupredoxins
[[Category: Unreleased Structures]]
[[Category: Weitz, A.C]]
[[Category: Hendrich, M.R]]
[[Category: Borovik, A.S]]
[[Category: Mann, S.I]]
[[Category: Ward, T.R]]
[[Category: Heinisch, T]]