5kf2 - Revision history

OCA at 09:59, 27 September 2023

2023-09-27T09:59:29Z

←Older revision		Revision as of 09:59, 27 September 2023
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	<StructureSection load='5kf2' size='340' side='right'caption='[[5kf2]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5kf2' size='340' side='right'caption='[[5kf2]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kf2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cloab Cloab~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KF2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KF2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kf2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_acetobutylicum_ATCC_824 Clostridium acetobutylicum ATCC 824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KF2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KF2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kf1\|5kf1]], [[5kf8\|5kf8]], [[5kf9\|5kf9]], [[5kga\|5kga]], [[5kgh\|5kgh]], [[5kgj\|5kgj]], [[5kgp\|5kgp]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CA_C0184 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272562 CLOAB])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kf2 OCA], [https://pdbe.org/5kf2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kf2 RCSB], [https://www.ebi.ac.uk/pdbsum/5kf2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kf2 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kf2 OCA], [~~http~~://pdbe.org/5kf2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kf2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kf2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kf2 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q97ML2_CLOAB Q97ML2_CLOAB]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cloab~~]]	+	[[Category: Clostridium acetobutylicum ATCC 824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dopkins~~, B J~~]]	+	[[Category: Dopkins BJ]]
-	[[Category: Holden~~, H M~~]]	+	[[Category: Holden HM]]
-	[[Category: Thoden~~, J B~~]]	+	[[Category: Thoden JB]]
-	[[Category: Tipton~~, P A]]~~	+	[[Category: Tipton PA]]
-	~~[[Category: Gnat]]~~	+
-	~~[[Category: N-acetyltransferase]]~~	+
-	~~[[Category: Tandem-gnat]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:11, 25 December 2019

2019-12-25T12:11:52Z

←Older revision		Revision as of 12:11, 25 December 2019
Line 1:		Line 1:

	==X-ray structure of a glucosamine N-Acetyltransferase from Clostridium acetobutylicum, apo form, pH 8==		==X-ray structure of a glucosamine N-Acetyltransferase from Clostridium acetobutylicum, apo form, pH 8==
-	<StructureSection load='5kf2' size='340' side='right' caption='[[5kf2]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5kf2' size='340' side='right'caption='[[5kf2]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5kf2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cloab Cloab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KF2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5kf2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cloab Cloab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KF2 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Cloab]]		[[Category: Cloab]]
		+	[[Category: Large Structures]]
	[[Category: Dopkins, B J]]		[[Category: Dopkins, B J]]
	[[Category: Holden, H M]]		[[Category: Holden, H M]]

OCA at 10:08, 15 November 2017

2017-11-15T10:08:54Z

←Older revision		Revision as of 10:08, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5kf2' size='340' side='right' caption='[[5kf2]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5kf2' size='340' side='right' caption='[[5kf2]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kf2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KF2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kf2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cloab Cloab]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KF2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kf1\|5kf1]], [[5kf8\|5kf8]], [[5kf9\|5kf9]], [[5kga\|5kga]], [[5kgh\|5kgh]], [[5kgj\|5kgj]], [[5kgp\|5kgp]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kf1\|5kf1]], [[5kf8\|5kf8]], [[5kf9\|5kf9]], [[5kga\|5kga]], [[5kgh\|5kgh]], [[5kgj\|5kgj]], [[5kgp\|5kgp]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CA_C0184 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272562 CLOAB])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kf2 OCA], [http://pdbe.org/5kf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kf2 RCSB], [http://www.ebi.ac.uk/pdbsum/5kf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kf2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kf2 OCA], [http://pdbe.org/5kf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kf2 RCSB], [http://www.ebi.ac.uk/pdbsum/5kf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kf2 ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cloab]]
	[[Category: Dopkins, B J]]		[[Category: Dopkins, B J]]
	[[Category: Holden, H M]]		[[Category: Holden, H M]]

OCA at 18:04, 13 July 2016

2016-07-13T18:04:19Z

←Older revision		Revision as of 18:04, 13 July 2016
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kf2 OCA], [http://pdbe.org/5kf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kf2 RCSB], [http://www.ebi.ac.uk/pdbsum/5kf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kf2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kf2 OCA], [http://pdbe.org/5kf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kf2 RCSB], [http://www.ebi.ac.uk/pdbsum/5kf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kf2 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glucosamine/glucosaminide N-acetyltransferase or GlmA catalyzes the transfer of an acetyl group from acetyl CoA to the primary amino group of glucosamine. The enzyme from Clostridium acetobutylicum is thought to be involved in cell wall rescue. In addition to glucosamine, GlmA has been shown to function on di- and trisaccharides of glucosamine as well. Here we present a structural and kinetic analysis of the enzyme. For this investigation, eight structures were determined to resolutions of 2.0 A or better. The overall three-dimensional fold of GlmA places it into the tandem GNAT superfamily. Each subunit of the dimer folds into two distinct domains which exhibit high three-dimensional structural similarity. Whereas both domains bind acetyl CoA, it is the C-terminal domain that is catalytically competent. On the basis of the various structures determined in this investigation, two amino acid residues were targeted for further study: Asp 287 and Tyr 297. Although their positions in the active site suggested that they may play key roles in catalysis by functioning as active site bases and acids, respectively, this was not borne out by characterization of the D287N and Y297F variants. The kinetic properties revealed that both residues were important for substrate binding, but had no critical roles as acid/base catalysts. Kinetic analyses also indicated that GlmA follows an ordered mechanism with acetyl CoA binding first followed by glucosamine. The product, N-acetylglucosamine is then released prior to CoA. The investigation described herein provides significantly new information on enzymes belonging to the tandem GNAT superfamily.
		+
		+	Structural Studies on a Glucosamine/Glucosaminide N-Acetyltransferase.,Dopkins BJ, Tipton PA, Thoden JB, Holden HM Biochemistry. 2016 Jun 27. PMID:27348258<ref>PMID:27348258</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5kf2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 03:03, 13 July 2016

2016-07-13T03:03:28Z

←Older revision		Revision as of 03:03, 13 July 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5kf2 is ON HOLD~~	+	==X-ray structure of a glucosamine N-Acetyltransferase from Clostridium acetobutylicum, apo form, pH 8==
-		+	<StructureSection load='5kf2' size='340' side='right' caption='[[5kf2]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors: Holden, H.M., Thoden, J.B., Dopkins, B.J., Tipton, P.A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5kf2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KF2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KF2 FirstGlance]. <br>
-	~~Description:~~ X-ray structure of a glucosamine N-Acetyltransferase from Clostridium acetobutylicum, apo form, pH 8	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kf1\|5kf1]], [[5kf8\|5kf8]], [[5kf9\|5kf9]], [[5kga\|5kga]], [[5kgh\|5kgh]], [[5kgj\|5kgj]], [[5kgp\|5kgp]]</td></tr>
-	[[~~Category~~: ~~Thoden~~, J.B]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kf2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kf2 OCA], [http://pdbe.org/5kf2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kf2 RCSB], [http://www.ebi.ac.uk/pdbsum/5kf2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kf2 ProSAT]</span></td></tr>
-	[[Category: Holden, H.M]]	+	</table>
-	[[Category: Tipton, P.A]]	+	__TOC__
-	[[Category: ~~Dopkins, B.J~~]]	+	</StructureSection>
		+	[[Category: Dopkins, B J]]
		+	[[Category: Holden, H M]]
		+	[[Category: Thoden, J B]]
		+	[[Category: Tipton, P A]]
		+	[[Category: Gnat]]
		+	[[Category: N-acetyltransferase]]
		+	[[Category: Tandem-gnat]]
		+	[[Category: Transferase]]

OCA: Protected "5kf2" [edit=sysop:move=sysop]

2016-06-23T21:32:34Z

Protected "5kf2" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:32, 23 June 2016

OCA: New page: '''Unreleased structure''' The entry 5kf2 is ON HOLD Authors: Holden, H.M., Thoden, J.B., Dopkins, B.J., Tipton, P.A. Description: X-ray structure of a glucosamine N-Acetyltransferase ...

2016-06-23T21:32:32Z

New page: '''Unreleased structure''' The entry 5kf2 is ON HOLD Authors: Holden, H.M., Thoden, J.B., Dopkins, B.J., Tipton, P.A. Description: X-ray structure of a glucosamine N-Acetyltransferase ...

New page

'''Unreleased structure'''

The entry 5kf2 is ON HOLD

Authors: Holden, H.M., Thoden, J.B., Dopkins, B.J., Tipton, P.A.

Description: X-ray structure of a glucosamine N-Acetyltransferase from Clostridium acetobutylicum, apo form, pH 8
[[Category: Unreleased Structures]]
[[Category: Thoden, J.B]]
[[Category: Holden, H.M]]
[[Category: Tipton, P.A]]
[[Category: Dopkins, B.J]]