5kfn - Revision history

OCA at 09:59, 27 September 2023

2023-09-27T09:59:53Z

←Older revision		Revision as of 09:59, 27 September 2023
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	<StructureSection load='5kfn' size='340' side='right'caption='[[5kfn]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='5kfn' size='340' side='right'caption='[[5kfn]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kfn]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KFN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KFN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kfn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KFN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STP:2-DEOXYADENOSINE+5-O-(1-THIOTRIPHOSPHATE)'>STP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kfa\|5kfa]], [[5kfb\|5kfb]], [[5kfc\|5kfc]], [[5kg0\|5kg0]], [[5kg1\|5kg1]], [[5kg2\|5kg2]], [[5kg3\|5kg3]], [[5kg4\|5kg4]], [[5kg5\|5kg5]], [[5kg6\|5kg6]], [[5kg7\|5kg7]], [[5kfd\|5kfd]], [[5kfe\|5kfe]], [[5kfs\|5kfs]], [[5kft\|5kft]], [[5kfu\|5kfu]], [[5kfv\|5kfv]], [[5kfw\|5kfw]], [[5kfx\|5kfx]], [[5kfg\|5kfg]], [[5kfy\|5kfy]], [[5kfz\|5kfz]], [[5kfh\|5kfh]], [[5kfk\|5kfk]], [[5kfl\|5kfl]], [[5kfi\|5kfi]], [[5kfj\|5kfj]], [[5kfm\|5kfm]], [[5kfo\|5kfo]], [[5kfp\|5kfp]], [[5kfq\|5kfq]], [[5kfr\|5kfr]], [[5kff\|5kff]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STP:2-DEOXYADENOSINE+5-O-(1-THIOTRIPHOSPHATE)'>STP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POLH, RAD30, RAD30A, XPV ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kfn OCA], [https://pdbe.org/5kfn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kfn RCSB], [https://www.ebi.ac.uk/pdbsum/5kfn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kfn ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kfn OCA], [~~http~~://pdbe.org/5kfn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kfn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kfn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kfn ProSAT]</span></td></tr>	+
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/POLH_HUMAN POLH_HUMAN]] Defects in POLH are the cause of xeroderma pigmentosum variant type (XPV) [MIM:[~~http~~://omim.org/entry/278750 278750]]; also designated as XP-V. Xeroderma pigmentosum (XP) is an autosomal recessive disease due to deficient nucleotide excision repair. It is characterized by hypersensitivity of the skin to sunlight, followed by high incidence of skin cancer and frequent neurologic abnormalities. XPV shows normal nucleotide excision repair, but an exaggerated delay in recovery of replicative DNA synthesis. Most XPV patients do not develop clinical symptoms and skin neoplasias until a later age. Clinical manifestations are limited to photo-induced deterioration of the skin and eyes.<ref>PMID:10385124</ref> <ref>PMID:10398605</ref> <ref>PMID:11032022</ref> <ref>PMID:11121129</ref> <ref>PMID:11773631</ref>	+	[https://www.uniprot.org/uniprot/POLH_HUMAN POLH_HUMAN] Defects in POLH are the cause of xeroderma pigmentosum variant type (XPV) [MIM:[https://omim.org/entry/278750 278750]; also designated as XP-V. Xeroderma pigmentosum (XP) is an autosomal recessive disease due to deficient nucleotide excision repair. It is characterized by hypersensitivity of the skin to sunlight, followed by high incidence of skin cancer and frequent neurologic abnormalities. XPV shows normal nucleotide excision repair, but an exaggerated delay in recovery of replicative DNA synthesis. Most XPV patients do not develop clinical symptoms and skin neoplasias until a later age. Clinical manifestations are limited to photo-induced deterioration of the skin and eyes.<ref>PMID:10385124</ref> <ref>PMID:10398605</ref> <ref>PMID:11032022</ref> <ref>PMID:11121129</ref> <ref>PMID:11773631</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/POLH_HUMAN POLH_HUMAN]] DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.<ref>PMID:10385124</ref> <ref>PMID:11743006</ref> <ref>PMID:11376341</ref> <ref>PMID:14630940</ref> <ref>PMID:14734526</ref>	+	[https://www.uniprot.org/uniprot/POLH_HUMAN POLH_HUMAN] DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.<ref>PMID:10385124</ref> <ref>PMID:11743006</ref> <ref>PMID:11376341</ref> <ref>PMID:14630940</ref> <ref>PMID:14734526</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~DNA-directed DNA polymerase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gao, Y]]	+	[[Category: Gao Y]]
-	[[Category: Yang, W]]	+	[[Category: Yang W]]
-	~~[[Category: Dna polymerase]]~~	+
-	~~[[Category: In crystallo reaction]]~~	+
-	~~[[Category: Metal ion dependent catalysis]]~~	+
-	~~[[Category: Replication]]~~	+
-	~~[[Category: Transferase-dna complex~~]]	+

OCA at 12:14, 25 December 2019

2019-12-25T12:14:20Z

←Older revision		Revision as of 12:14, 25 December 2019
Line 1:		Line 1:

	==Human DNA polymerase eta-DNA ternary complex with Sp-dATP-alpha-S: reaction with 1 mM Mg2+ for 1800s==		==Human DNA polymerase eta-DNA ternary complex with Sp-dATP-alpha-S: reaction with 1 mM Mg2+ for 1800s==
-	<StructureSection load='5kfn' size='340' side='right' caption='[[5kfn]], [[Resolution\|resolution]] 1.45Å' scene=''>	+	<StructureSection load='5kfn' size='340' side='right'caption='[[5kfn]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5kfn]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KFN FirstGlance]. <br>		<table><tr><td colspan='2'>[[5kfn]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KFN FirstGlance]. <br>
Line 23:		Line 23:
	</div>		</div>
	<div class="pdbe-citations 5kfn" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5kfn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 29:		Line 32:
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]
	[[Category: Yang, W]]		[[Category: Yang, W]]

OCA at 18:16, 15 November 2017

2017-11-15T18:16:26Z

←Older revision		Revision as of 18:16, 15 November 2017
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	<StructureSection load='5kfn' size='340' side='right' caption='[[5kfn]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='5kfn' size='340' side='right' caption='[[5kfn]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kfn]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KFN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kfn]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KFN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STP:2-DEOXYADENOSINE+5-O-(1-THIOTRIPHOSPHATE)'>STP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STP:2-DEOXYADENOSINE+5-O-(1-THIOTRIPHOSPHATE)'>STP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kfa\|5kfa]], [[5kfb\|5kfb]], [[5kfc\|5kfc]], [[5kg0\|5kg0]], [[5kg1\|5kg1]], [[5kg2\|5kg2]], [[5kg3\|5kg3]], [[5kg4\|5kg4]], [[5kg5\|5kg5]], [[5kg6\|5kg6]], [[5kg7\|5kg7]], [[5kfd\|5kfd]], [[5kfe\|5kfe]], [[5kfs\|5kfs]], [[5kft\|5kft]], [[5kfu\|5kfu]], [[5kfv\|5kfv]], [[5kfw\|5kfw]], [[5kfx\|5kfx]], [[5kfg\|5kfg]], [[5kfy\|5kfy]], [[5kfz\|5kfz]], [[5kfh\|5kfh]], [[5kfk\|5kfk]], [[5kfl\|5kfl]], [[5kfi\|5kfi]], [[5kfj\|5kfj]], [[5kfm\|5kfm]], [[5kfo\|5kfo]], [[5kfp\|5kfp]], [[5kfq\|5kfq]], [[5kfr\|5kfr]], [[5kff\|5kff]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kfa\|5kfa]], [[5kfb\|5kfb]], [[5kfc\|5kfc]], [[5kg0\|5kg0]], [[5kg1\|5kg1]], [[5kg2\|5kg2]], [[5kg3\|5kg3]], [[5kg4\|5kg4]], [[5kg5\|5kg5]], [[5kg6\|5kg6]], [[5kg7\|5kg7]], [[5kfd\|5kfd]], [[5kfe\|5kfe]], [[5kfs\|5kfs]], [[5kft\|5kft]], [[5kfu\|5kfu]], [[5kfv\|5kfv]], [[5kfw\|5kfw]], [[5kfx\|5kfx]], [[5kfg\|5kfg]], [[5kfy\|5kfy]], [[5kfz\|5kfz]], [[5kfh\|5kfh]], [[5kfk\|5kfk]], [[5kfl\|5kfl]], [[5kfi\|5kfi]], [[5kfj\|5kfj]], [[5kfm\|5kfm]], [[5kfo\|5kfo]], [[5kfp\|5kfp]], [[5kfq\|5kfq]], [[5kfr\|5kfr]], [[5kff\|5kff]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POLH, RAD30, RAD30A, XPV ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kfn OCA], [http://pdbe.org/5kfn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kfn RCSB], [http://www.ebi.ac.uk/pdbsum/5kfn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kfn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kfn OCA], [http://pdbe.org/5kfn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kfn RCSB], [http://www.ebi.ac.uk/pdbsum/5kfn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kfn ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: DNA-directed DNA polymerase]]		[[Category: DNA-directed DNA polymerase]]
		+	[[Category: Human]]
	[[Category: Gao, Y]]		[[Category: Gao, Y]]
	[[Category: Yang, W]]		[[Category: Yang, W]]

OCA at 02:29, 13 July 2016

2016-07-13T02:29:39Z

←Older revision		Revision as of 02:29, 13 July 2016
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kfa\|5kfa]], [[5kfb\|5kfb]], [[5kfc\|5kfc]], [[5kg0\|5kg0]], [[5kg1\|5kg1]], [[5kg2\|5kg2]], [[5kg3\|5kg3]], [[5kg4\|5kg4]], [[5kg5\|5kg5]], [[5kg6\|5kg6]], [[5kg7\|5kg7]], [[5kfd\|5kfd]], [[5kfe\|5kfe]], [[5kfs\|5kfs]], [[5kft\|5kft]], [[5kfu\|5kfu]], [[5kfv\|5kfv]], [[5kfw\|5kfw]], [[5kfx\|5kfx]], [[5kfg\|5kfg]], [[5kfy\|5kfy]], [[5kfz\|5kfz]], [[5kfh\|5kfh]], [[5kfk\|5kfk]], [[5kfl\|5kfl]], [[5kfi\|5kfi]], [[5kfj\|5kfj]], [[5kfm\|5kfm]], [[5kfo\|5kfo]], [[5kfp\|5kfp]], [[5kfq\|5kfq]], [[5kfr\|5kfr]], [[5kff\|5kff]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kfa\|5kfa]], [[5kfb\|5kfb]], [[5kfc\|5kfc]], [[5kg0\|5kg0]], [[5kg1\|5kg1]], [[5kg2\|5kg2]], [[5kg3\|5kg3]], [[5kg4\|5kg4]], [[5kg5\|5kg5]], [[5kg6\|5kg6]], [[5kg7\|5kg7]], [[5kfd\|5kfd]], [[5kfe\|5kfe]], [[5kfs\|5kfs]], [[5kft\|5kft]], [[5kfu\|5kfu]], [[5kfv\|5kfv]], [[5kfw\|5kfw]], [[5kfx\|5kfx]], [[5kfg\|5kfg]], [[5kfy\|5kfy]], [[5kfz\|5kfz]], [[5kfh\|5kfh]], [[5kfk\|5kfk]], [[5kfl\|5kfl]], [[5kfi\|5kfi]], [[5kfj\|5kfj]], [[5kfm\|5kfm]], [[5kfo\|5kfo]], [[5kfp\|5kfp]], [[5kfq\|5kfq]], [[5kfr\|5kfr]], [[5kff\|5kff]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kfn OCA], [http://pdbe.org/5kfn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kfn RCSB], [http://www.ebi.ac.uk/pdbsum/5kfn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kfn OCA], [http://pdbe.org/5kfn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kfn RCSB], [http://www.ebi.ac.uk/pdbsum/5kfn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kfn ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA: Protected "5kfn" [edit=sysop:move=sysop]

2016-06-23T23:08:33Z

Protected "5kfn" [edit=sysop:move=sysop]

←Older revision

Revision as of 23:08, 23 June 2016

OCA: New page: ==Human DNA polymerase eta-DNA ternary complex with Sp-dATP-alpha-S: reaction with 1 mM Mg2+ for 1800s== == Structural highlights ==
<table><tr><td colspan='2'>[[5kfn]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KFN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KFN FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STP:2-DEOXYADENOSINE+5-O-(1-THIOTRIPHOSPHATE)'>STP</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5kfa|5kfa]], [[5kfb|5kfb]], [[5kfc|5kfc]], [[5kg0|5kg0]], [[5kg1|5kg1]], [[5kg2|5kg2]], [[5kg3|5kg3]], [[5kg4|5kg4]], [[5kg5|5kg5]], [[5kg6|5kg6]], [[5kg7|5kg7]], [[5kfd|5kfd]], [[5kfe|5kfe]], [[5kfs|5kfs]], [[5kft|5kft]], [[5kfu|5kfu]], [[5kfv|5kfv]], [[5kfw|5kfw]], [[5kfx|5kfx]], [[5kfg|5kfg]], [[5kfy|5kfy]], [[5kfz|5kfz]], [[5kfh|5kfh]], [[5kfk|5kfk]], [[5kfl|5kfl]], [[5kfi|5kfi]], [[5kfj|5kfj]], [[5kfm|5kfm]], [[5kfo|5kfo]], [[5kfp|5kfp]], [[5kfq|5kfq]], [[5kfr|5kfr]], [[5kff|5kff]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kfn OCA], [http://pdbe.org/5kfn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kfn RCSB], [http://www.ebi.ac.uk/pdbsum/5kfn PDBsum]</span></td></tr>
</table>
== Disease ==
[[http://www.uniprot.org/uniprot/POLH_HUMAN POLH_HUMAN]] Defects in POLH are the cause of xeroderma pigmentosum variant type (XPV) [MIM:[http://omim.org/entry/278750 278750]]; also designated as XP-V. Xeroderma pigmentosum (XP) is an autosomal recessive disease due to deficient nucleotide excision repair. It is characterized by hypersensitivity of the skin to sunlight, followed by high incidence of skin cancer and frequent neurologic abnormalities. XPV shows normal nucleotide excision repair, but an exaggerated delay in recovery of replicative DNA synthesis. Most XPV patients do not develop clinical symptoms and skin neoplasias until a later age. Clinical manifestations are limited to photo-induced deterioration of the skin and eyes.<ref>PMID:10385124</ref> <ref>PMID:10398605</ref> <ref>PMID:11032022</ref> <ref>PMID:11121129</ref> <ref>PMID:11773631</ref>
== Function ==
[[http://www.uniprot.org/uniprot/POLH_HUMAN POLH_HUMAN]] DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI to replication foci.<ref>PMID:10385124</ref> <ref>PMID:11743006</ref> <ref>PMID:11376341</ref> <ref>PMID:14630940</ref> <ref>PMID:14734526</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
It is generally assumed that an enzyme-substrate (ES) complex contains all components necessary for catalysis and that conversion to products occurs by rearrangement of atoms, protons, and electrons. However, we find that DNA synthesis does not occur in a fully assembled DNA polymerase-DNA-deoxynucleoside triphosphate complex with two canonical metal ions bound. Using time-resolved x-ray crystallography, we show that the phosphoryltransfer reaction takes place only after the ES complex captures a third divalent cation that is not coordinated by the enzyme. Binding of the third cation is incompatible with the basal ES complex and requires thermal activation of the ES for entry. It is likely that the third cation provides the ultimate boost over the energy barrier to catalysis of DNA synthesis.

Capture of a third Mg(2)(+) is essential for catalyzing DNA synthesis.,Gao Y, Yang W Science. 2016 Jun 10;352(6291):1334-7. doi: 10.1126/science.aad9633. PMID:27284197<ref>PMID:27284197</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5kfn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: DNA-directed DNA polymerase]]
[[Category: Gao, Y]]
[[Category: Yang, W]]
[[Category: Dna polymerase]]
[[Category: In crystallo reaction]]
[[Category: Metal ion dependent catalysis]]
[[Category: Replication]]
[[Category: Transferase-dna complex]]