5kk9 - Revision history

OCA at 10:48, 27 September 2023

2023-09-27T10:48:14Z

←Older revision		Revision as of 10:48, 27 September 2023
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	==Connexin 32 G12R N-Terminal Mutant,==		==Connexin 32 G12R N-Terminal Mutant,==
-	<StructureSection load='5kk9' size='340' side='right'caption='[[5kk9~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5kk9' size='340' side='right'caption='[[5kk9]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kk9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KK9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kk9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KK9 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk9 OCA], [~~http~~://pdbe.org/5kk9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kk9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kk9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk9 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk9 OCA], [https://pdbe.org/5kk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kk9 RCSB], [https://www.ebi.ac.uk/pdbsum/5kk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk9 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/CXB1_HUMAN CXB1_HUMAN]] X-linked Charcot-Marie-Tooth disease type 1;X-linked progressive cerebellar ataxia. The disease is caused by mutations affecting the gene represented in this entry. The gene represented in this entry may act as a disease modifier.	+	[https://www.uniprot.org/uniprot/CXB1_HUMAN CXB1_HUMAN] X-linked Charcot-Marie-Tooth disease type 1;X-linked progressive cerebellar ataxia. The disease is caused by mutations affecting the gene represented in this entry. The gene represented in this entry may act as a disease modifier.
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CXB1_HUMAN CXB1_HUMAN]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.	+	[https://www.uniprot.org/uniprot/CXB1_HUMAN CXB1_HUMAN] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Barigello~~, T A~~]]	+	[[Category: Barigello TA]]
-	[[Category: Dowd~~, T L]]~~	+	[[Category: Dowd TL]]
-	~~[[Category: Connexin 32 g12r n-terminal mutant]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 12:18, 25 December 2019

2019-12-25T12:18:13Z

←Older revision		Revision as of 12:18, 25 December 2019
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	==Connexin 32 G12R N-Terminal Mutant,==		==Connexin 32 G12R N-Terminal Mutant,==
-	<StructureSection load='5kk9' size='340' side='right' caption='[[5kk9]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5kk9' size='340' side='right'caption='[[5kk9]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5kk9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KK9 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5kk9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KK9 FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 5kk9" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5kk9" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Connexin 3D structure\|Connexin 3D structure]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Barigello, T A]]		[[Category: Barigello, T A]]
	[[Category: Dowd, T L]]		[[Category: Dowd, T L]]
	[[Category: Connexin 32 g12r n-terminal mutant]]		[[Category: Connexin 32 g12r n-terminal mutant]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 16:48, 3 October 2016

2016-10-03T16:48:19Z

←Older revision		Revision as of 16:48, 3 October 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5kk9 is ~~ON HOLD~~ ~~until sometime~~ in the ~~future~~	+	==Connexin 32 G12R N-Terminal Mutant,==
		+	<StructureSection load='5kk9' size='340' side='right' caption='[[5kk9]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kk9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KK9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KK9 FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kk9 OCA], [http://pdbe.org/5kk9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kk9 RCSB], [http://www.ebi.ac.uk/pdbsum/5kk9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kk9 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/CXB1_HUMAN CXB1_HUMAN]] X-linked Charcot-Marie-Tooth disease type 1;X-linked progressive cerebellar ataxia. The disease is caused by mutations affecting the gene represented in this entry. The gene represented in this entry may act as a disease modifier.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CXB1_HUMAN CXB1_HUMAN]] One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Alterations in gap junctions underlie the etiologies of syndromic deafness (KID) and Charcot-Marie Tooth disease (CMTX). Functional gap junctions are composed of connexin molecules with N-termini containing a flexible turn around G12, inserting the N-termini into the channel pore allowing voltage gating. The loss of this turn correlates with loss of Connexin 32 (Cx32) function by impaired trafficking to the cell membrane. Using (1)H NMR we show the N-terminus of a syndromic deafness mutation Cx26G12R, producing "leaky channels", contains a turn around G12 which is less structured and more flexible than wild-type. In contrast, the N-terminal structure of the same mutation in Cx32 chimera, Cx32*43E1G12R shows a larger constricted turn and no membrane current expression but forms membrane inserted hemichannels. Their function was rescued by formation of heteromeric channels with wild type subunits. We suggest the inflexible Cx32G12R N-terminus blocks ion conduction in homomeric channels and this channel block is relieved by incorporation of wild type subunits. In contrast, the increased open probability of Cx26G12R hemichannels is likely due to the addition of positive charge in the channel pore changing pore electrostatics and impairing hemichannel regulation by Ca(2+). These results provide mechanistic information on aberrant channel activity observed in disease.

-	~~Authors~~:	+	Structural studies of N-terminal mutants of Connexin 26 and Connexin 32 using (1)H NMR spectroscopy.,Batir Y, Bargiello TA, Dowd TL Arch Biochem Biophys. 2016 Oct 15;608:8-19. doi: 10.1016/j.abb.2016.06.019. Epub , 2016 Jul 1. PMID:27378082<ref>PMID:27378082</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5kk9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Barigello, T A]]
		+	[[Category: Dowd, T L]]
		+	[[Category: Connexin 32 g12r n-terminal mutant]]
		+	[[Category: Transport protein]]

OCA at 14:04, 9 September 2016

2016-09-09T14:04:45Z

←Older revision		Revision as of 14:04, 9 September 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kk9 is ON HOLD	+	The entry 5kk9 is ON HOLD until sometime in the future

	Authors:		Authors:

OCA: Protected "5kk9" [edit=sysop:move=sysop]

2016-07-13T00:28:14Z

Protected "5kk9" [edit=sysop:move=sysop]

←Older revision

Revision as of 00:28, 13 July 2016

OCA: New page: '''Unreleased structure''' The entry 5kk9 is ON HOLD Authors: Description: Category: Unreleased Structures

2016-07-13T00:28:13Z

New page: '''Unreleased structure''' The entry 5kk9 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5kk9 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]