5kp7 - Revision history

OCA at 10:50, 27 September 2023

2023-09-27T10:50:31Z

←Older revision		Revision as of 10:50, 27 September 2023
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	<StructureSection load='5kp7' size='340' side='right'caption='[[5kp7]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5kp7' size='340' side='right'caption='[[5kp7]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kp7]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula] and [~~http~~://en.wikipedia.org/wiki/~~Lyngbya_majuscula_3l Lyngbya majuscula 3l~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KP7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KP7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kp7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula] and [https://en.wikipedia.org/wiki/Moorena_producens_3L Moorena producens 3L]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KP7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=PNS:4~~-~~PHOSPHOPANTETHEINE'>PNS</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>LYNGBM3L_74540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~489825 Lyngbya majuscula 3L]), curB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158786 Lyngbya majuscula])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Hydroxymethylglutaryl-CoA_synthase Hydroxymethylglutaryl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.10 2.3.3.10]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kp7 OCA], [https://pdbe.org/5kp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kp7 RCSB], [https://www.ebi.ac.uk/pdbsum/5kp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kp7 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kp7 OCA], [~~http~~://pdbe.org/5kp7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kp7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kp7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kp7 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/F4Y432_9CYAN F4Y432_9CYAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hydroxymethylglutaryl-CoA synthase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lyngbya majuscula]]		[[Category: Lyngbya majuscula]]
-	[[Category: ~~Lyngbya majuscula 3l~~]]	+	[[Category: Moorena producens 3L]]
-	[[Category: Maloney~~, F P~~]]	+	[[Category: Maloney FP]]
-	[[Category: Smith~~, J L]]~~	+	[[Category: Smith JL]]
-	~~[[Category: Acyl carrier protein]]~~	+
-	~~[[Category: Enzyme-acp complex]]~~	+
-	~~[[Category: Hmg synthase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:15, 4 December 2019

2019-12-04T09:15:17Z

←Older revision		Revision as of 09:15, 4 December 2019
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	==Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with Holo Donor-ACP==		==Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with Holo Donor-ACP==
-	<StructureSection load='5kp7' size='340' side='right' caption='[[5kp7]], [[Resolution\|resolution]] 1.60Å' scene=''>	+	<StructureSection load='5kp7' size='340' side='right'caption='[[5kp7]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5kp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula] and [http://en.wikipedia.org/wiki/Lyngbya_majuscula_3l Lyngbya majuscula 3l]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KP7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5kp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula] and [http://en.wikipedia.org/wiki/Lyngbya_majuscula_3l Lyngbya majuscula 3l]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KP7 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Hydroxymethylglutaryl-CoA synthase]]		[[Category: Hydroxymethylglutaryl-CoA synthase]]
		+	[[Category: Large Structures]]
	[[Category: Lyngbya majuscula]]		[[Category: Lyngbya majuscula]]
	[[Category: Lyngbya majuscula 3l]]		[[Category: Lyngbya majuscula 3l]]

OCA at 18:27, 15 November 2017

2017-11-15T18:27:07Z

←Older revision		Revision as of 18:27, 15 November 2017
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	<StructureSection load='5kp7' size='340' side='right' caption='[[5kp7]], [[Resolution\|resolution]] 1.60Å' scene=''>		<StructureSection load='5kp7' size='340' side='right' caption='[[5kp7]], [[Resolution\|resolution]] 1.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kp7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KP7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kp7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula] and [http://en.wikipedia.org/wiki/Lyngbya_majuscula_3l Lyngbya majuscula 3l]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KP7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LYNGBM3L_74540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=489825 Lyngbya majuscula 3L]), curB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158786 Lyngbya majuscula])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydroxymethylglutaryl-CoA_synthase Hydroxymethylglutaryl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.10 2.3.3.10] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydroxymethylglutaryl-CoA_synthase Hydroxymethylglutaryl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.10 2.3.3.10] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kp7 OCA], [http://pdbe.org/5kp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kp7 RCSB], [http://www.ebi.ac.uk/pdbsum/5kp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kp7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kp7 OCA], [http://pdbe.org/5kp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kp7 RCSB], [http://www.ebi.ac.uk/pdbsum/5kp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kp7 ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Hydroxymethylglutaryl-CoA synthase]]		[[Category: Hydroxymethylglutaryl-CoA synthase]]
		+	[[Category: Lyngbya majuscula]]
		+	[[Category: Lyngbya majuscula 3l]]
	[[Category: Maloney, F P]]		[[Category: Maloney, F P]]
	[[Category: Smith, J L]]		[[Category: Smith, J L]]

OCA at 08:12, 14 September 2016

2016-09-14T08:12:45Z

←Older revision		Revision as of 08:12, 14 September 2016
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kp7 OCA], [http://pdbe.org/5kp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kp7 RCSB], [http://www.ebi.ac.uk/pdbsum/5kp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kp7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kp7 OCA], [http://pdbe.org/5kp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kp7 RCSB], [http://www.ebi.ac.uk/pdbsum/5kp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kp7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Alkyl branching at the beta position of a polyketide intermediate is an important variation on canonical polyketide natural product biosynthesis. The branching enzyme, 3-hydroxy-3-methylglutaryl synthase (HMGS), catalyzes the aldol addition of an acyl donor to a beta-keto-polyketide intermediate acceptor. HMGS is highly selective for two specialized acyl carrier proteins (ACPs) that deliver the donor and acceptor substrates. The HMGS from the curacin A biosynthetic pathway (CurD) was examined to establish the basis for ACP selectivity. The donor ACP (CurB) had high affinity for the enzyme (Kd = 0.5 muM) and could not be substituted by the acceptor ACP. High-resolution crystal structures of HMGS alone and in complex with its donor ACP reveal a tight interaction that depends on exquisite surface shape and charge complementarity between the proteins. Selectivity is explained by HMGS binding to an unusual surface cleft on the donor ACP, in a manner that would exclude the acceptor ACP. Within the active site, HMGS discriminates between pre- and postreaction states of the donor ACP. The free phosphopantetheine (Ppant) cofactor of ACP occupies a conserved pocket that excludes the acetyl-Ppant substrate. In comparison with HMG-CoA (CoA) synthase, the homologous enzyme from primary metabolism, HMGS has several differences at the active site entrance, including a flexible-loop insertion, which may account for the specificity of one enzyme for substrates delivered by ACP and the other by CoA.
		+
		+	Anatomy of the beta-branching enzyme of polyketide biosynthesis and its interaction with an acyl-ACP substrate.,Maloney FP, Gerwick L, Gerwick WH, Sherman DH, Smith JL Proc Natl Acad Sci U S A. 2016 Aug 29. pii: 201607210. PMID:27573844<ref>PMID:27573844</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5kp7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 13:58, 10 September 2016

2016-09-10T13:58:43Z

←Older revision		Revision as of 13:58, 10 September 2016
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-	'''Unreleased structure'''

-	~~The entry 5kp7 is ON HOLD until Paper Publication~~	+	==Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with Holo Donor-ACP==
-		+	<StructureSection load='5kp7' size='340' side='right' caption='[[5kp7]], [[Resolution\|resolution]] 1.60Å' scene=''>
-	~~Authors: Maloney, F.P., Smith, J.L.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5kp7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KP7 FirstGlance]. <br>
-	~~Description:~~ Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with Holo Donor-ACP	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydroxymethylglutaryl-CoA_synthase Hydroxymethylglutaryl-CoA synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.10 2.3.3.10] </span></td></tr>
-	[[~~Category~~: ~~Smith~~, J.L]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kp7 OCA], [http://pdbe.org/5kp7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kp7 RCSB], [http://www.ebi.ac.uk/pdbsum/5kp7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kp7 ProSAT]</span></td></tr>
-	[[Category: Maloney, F.P]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hydroxymethylglutaryl-CoA synthase]]
		+	[[Category: Maloney, F P]]
		+	[[Category: Smith, J L]]
		+	[[Category: Acyl carrier protein]]
		+	[[Category: Enzyme-acp complex]]
		+	[[Category: Hmg synthase]]
		+	[[Category: Transferase]]

OCA at 13:12, 27 July 2016

2016-07-27T13:12:45Z

←Older revision		Revision as of 13:12, 27 July 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kp7 is ON HOLD	+	The entry 5kp7 is ON HOLD until Paper Publication

	Authors: Maloney, F.P., Smith, J.L.		Authors: Maloney, F.P., Smith, J.L.

OCA: Protected "5kp7" [edit=sysop:move=sysop]

2016-07-14T09:32:01Z

Protected "5kp7" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:32, 14 July 2016

OCA: New page: '''Unreleased structure''' The entry 5kp7 is ON HOLD Authors: Maloney, F.P., Smith, J.L. Description: Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with...

2016-07-14T09:32:00Z

New page: '''Unreleased structure''' The entry 5kp7 is ON HOLD Authors: Maloney, F.P., Smith, J.L. Description: Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with...

New page

'''Unreleased structure'''

The entry 5kp7 is ON HOLD

Authors: Maloney, F.P., Smith, J.L.

Description: Crystal Structure of the Curacin Biosynthetic Pathway HMG Synthase in Complex with Holo Donor-ACP
[[Category: Unreleased Structures]]
[[Category: Smith, J.L]]
[[Category: Maloney, F.P]]