5kug - Revision history

OCA at 10:55, 27 September 2023

2023-09-27T10:55:06Z

←Older revision		Revision as of 10:55, 27 September 2023
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	<StructureSection load='5kug' size='340' side='right'caption='[[5kug]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5kug' size='340' side='right'caption='[[5kug]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kug]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kug]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KUG FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5kuj\|5kuj]], [[5kui\|5kui]], [[5kue\|5kue]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">MCU, C10orf42, CCDC109A ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kug OCA], [https://pdbe.org/5kug PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kug RCSB], [https://www.ebi.ac.uk/pdbsum/5kug PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kug ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kug OCA], [~~http~~://pdbe.org/5kug PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kug RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kug PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kug ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MCU_HUMAN MCU_HUMAN]] Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Activity is regulated by MICU1 and MICU2 that stimulate and inhibit MCU activity, respectively. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes.<ref>PMID:21685886</ref> <ref>PMID:21685888</ref> <ref>PMID:22822213</ref> <ref>PMID:22829870</ref> <ref>PMID:22904319</ref> <ref>PMID:23101630</ref> <ref>PMID:23178883</ref> <ref>PMID:24332854</ref> <ref>PMID:24560927</ref>	+	[https://www.uniprot.org/uniprot/MCU_HUMAN MCU_HUMAN] Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Activity is regulated by MICU1 and MICU2 that stimulate and inhibit MCU activity, respectively. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes.<ref>PMID:21685886</ref> <ref>PMID:21685888</ref> <ref>PMID:22822213</ref> <ref>PMID:22829870</ref> <ref>PMID:22904319</ref> <ref>PMID:23101630</ref> <ref>PMID:23178883</ref> <ref>PMID:24332854</ref> <ref>PMID:24560927</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5kug" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5kug" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Mitochondrial calcium uniporter\|Mitochondrial calcium uniporter]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Junop~~, M S~~]]	+	[[Category: Junop MS]]
-	[[Category: Lee~~, S K~~]]	+	[[Category: Lee SK]]
-	[[Category: Mok~~, M C.Y~~]]	+	[[Category: Mok MCY]]
-	[[Category: Stathopulos~~, P B]]~~	+	[[Category: Stathopulos PB]]
-	~~[[Category: Beta-grasp fold n-terminal domain residues 72-189 mitochondrial calcium uniporter]]~~	+
-	~~[[Category: Metal transport~~]]	+

OCA at 08:11, 8 January 2020

2020-01-08T08:11:43Z

←Older revision		Revision as of 08:11, 8 January 2020
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	==Human mitochondrial calcium uniporter (residues 72-189) crystal structure with lithium==		==Human mitochondrial calcium uniporter (residues 72-189) crystal structure with lithium==
-	<StructureSection load='5kug' size='340' side='right' caption='[[5kug]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='5kug' size='340' side='right'caption='[[5kug]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KUG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kug]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KUG FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kuj\|5kuj]], [[5kui\|5kui]], [[5kue\|5kue]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kuj\|5kuj]], [[5kui\|5kui]], [[5kue\|5kue]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MCU, C10orf42, CCDC109A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kug OCA], [http://pdbe.org/5kug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kug RCSB], [http://www.ebi.ac.uk/pdbsum/5kug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kug ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kug OCA], [http://pdbe.org/5kug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kug RCSB], [http://www.ebi.ac.uk/pdbsum/5kug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kug ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Junop, M S]]		[[Category: Junop, M S]]
	[[Category: Lee, S K]]		[[Category: Lee, S K]]

OCA at 21:12, 10 September 2016

2016-09-10T21:12:54Z

←Older revision		Revision as of 21:12, 10 September 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5kug is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Human mitochondrial calcium uniporter (residues 72-189) crystal structure with lithium==
		+	<StructureSection load='5kug' size='340' side='right' caption='[[5kug]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kug]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KUG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KUG FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kuj\|5kuj]], [[5kui\|5kui]], [[5kue\|5kue]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kug FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kug OCA], [http://pdbe.org/5kug PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kug RCSB], [http://www.ebi.ac.uk/pdbsum/5kug PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kug ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCU_HUMAN MCU_HUMAN]] Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Activity is regulated by MICU1 and MICU2 that stimulate and inhibit MCU activity, respectively. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes.<ref>PMID:21685886</ref> <ref>PMID:21685888</ref> <ref>PMID:22822213</ref> <ref>PMID:22829870</ref> <ref>PMID:22904319</ref> <ref>PMID:23101630</ref> <ref>PMID:23178883</ref> <ref>PMID:24332854</ref> <ref>PMID:24560927</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Calcium (Ca2+) flux into the matrix is tightly controlled by the mitochondrial Ca2+ uniporter (MCU) due to vital roles in cell death and bioenergetics. However, the precise atomic mechanisms of MCU regulation remain unclear. Here, we solved the crystal structure of the N-terminal matrix domain of human MCU, revealing a beta-grasp-like fold with a cluster of negatively charged residues that interacts with divalent cations. Binding of Ca2+ or Mg2+ destabilizes and shifts the self-association equilibrium of the domain toward monomer. Mutational disruption of the acidic face weakens oligomerization of the isolated matrix domain and full-length human protein similar to cation binding and markedly decreases MCU activity. Moreover, mitochondrial Mg2+ loading or blockade of mitochondrial Ca2+ extrusion suppresses MCU Ca2+-uptake rates. Collectively, our data reveal that the beta-grasp-like matrix region harbors an MCU-regulating acidic patch that inhibits human MCU activity in response to Mg2+ and Ca2+ binding.

-	~~Authors: Mok, M.C.Y~~., Lee, S~~.K.~~, Junop, M.S., ~~Stathopulos, P~~.B.	+	Structural Insights into Mitochondrial Calcium Uniporter Regulation by Divalent Cations.,Lee SK, Shanmughapriya S, Mok MC, Dong Z, Tomar D, Carvalho E, Rajan S, Junop MS, Madesh M, Stathopulos PB Cell Chem Biol. 2016 Aug 24. pii: S2451-9456(16)30242-2. doi:, 10.1016/j.chembiol.2016.07.012. PMID:27569754<ref>PMID:27569754</ref>

-	~~Description: Human mitochondrial calcium uniporter (residues 72-189) crystal structure with lithium~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Lee~~, S.K]]	+	<div class="pdbe-citations 5kug" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Junop~~, M.S]]	+	== References ==
-	[[Category: Mok, M.C.Y]]	+	<references/>
-	[[Category: Stathopulos, P.B]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Junop, M S]]
		+	[[Category: Lee, S K]]
		+	[[Category: Mok, M C.Y]]
		+	[[Category: Stathopulos, P B]]
		+	[[Category: Beta-grasp fold n-terminal domain residues 72-189 mitochondrial calcium uniporter]]
		+	[[Category: Metal transport]]

OCA at 13:22, 27 July 2016

2016-07-27T13:22:33Z

←Older revision		Revision as of 13:22, 27 July 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kug is ON HOLD	+	The entry 5kug is ON HOLD until Paper Publication

	Authors: Mok, M.C.Y., Lee, S.K., Junop, M.S., Stathopulos, P.B.		Authors: Mok, M.C.Y., Lee, S.K., Junop, M.S., Stathopulos, P.B.

-	Description: Human mitochondrial calcium uniporter (residues 72-189) crystal structure with lithium ~~sulfate.~~	+	Description: Human mitochondrial calcium uniporter (residues 72-189) crystal structure with lithium
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Lee, S.K]]		[[Category: Lee, S.K]]

OCA: Protected "5kug" [edit=sysop:move=sysop]

2016-07-26T12:56:37Z

Protected "5kug" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:56, 26 July 2016

OCA: New page: '''Unreleased structure''' The entry 5kug is ON HOLD Authors: Mok, M.C.Y., Lee, S.K., Junop, M.S., Stathopulos, P.B. Description: Human mitochondrial calcium uniporter (residues 72-189...

2016-07-26T12:56:36Z

New page: '''Unreleased structure''' The entry 5kug is ON HOLD Authors: Mok, M.C.Y., Lee, S.K., Junop, M.S., Stathopulos, P.B. Description: Human mitochondrial calcium uniporter (residues 72-189...

New page

'''Unreleased structure'''

The entry 5kug is ON HOLD

Authors: Mok, M.C.Y., Lee, S.K., Junop, M.S., Stathopulos, P.B.

Description: Human mitochondrial calcium uniporter (residues 72-189) crystal structure with lithium sulfate.
[[Category: Unreleased Structures]]
[[Category: Lee, S.K]]
[[Category: Junop, M.S]]
[[Category: Mok, M.C.Y]]
[[Category: Stathopulos, P.B]]