5kvs - Revision history

OCA at 10:57, 27 September 2023

2023-09-27T10:57:49Z

←Older revision		Revision as of 10:57, 27 September 2023
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	<StructureSection load='5kvs' size='340' side='right'caption='[[5kvs]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='5kvs' size='340' side='right'caption='[[5kvs]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kvs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacterium_enterocoliticum"_schleifstein_and_coleman_1939 "bacterium enterocoliticum" schleifstein and coleman 1939~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KVS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5KVS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kvs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KVS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6XR:(4~{R})-2-[2-(2-HYDROXYPHENYL)-1,3-THIAZOL-4-YL]-4,5-DIHYDRO-1,3-THIAZOLE-4-CARBOXYLIC+ACID'>6XR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.28Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kvq\|5kvq]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6XR:(4~{R})-2-[2-(2-HYDROXYPHENYL)-1,3-THIAZOL-4-YL]-4,5-DIHYDRO-1,3-THIAZOLE-4-CARBOXYLIC+ACID'>6XR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">irp3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 "Bacterium enterocoliticum" Schleifstein and Coleman 1939])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kvs OCA], [https://pdbe.org/5kvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kvs RCSB], [https://www.ebi.ac.uk/pdbsum/5kvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kvs ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5kvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kvs OCA], [~~http~~://pdbe.org/5kvs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5kvs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5kvs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5kvs ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O54512_YEREN O54512_YEREN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacterium enterocoliticum schleifstein and coleman 1939]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lamb, A L~~]]	+	[[Category: Yersinia enterocolitica]]
-	[[Category: ~~Meneely, K M~~]]	+	[[Category: Lamb AL]]
-	[[Category: ~~Hptt-cooh]]~~	+	[[Category: Meneely KM]]
-	~~[[Category: Imine reductase]]~~	+
-	~~[[Category: Irp3]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Siderophore]]~~	+
-	~~[[Category: Substrate analog]]~~	+
-	~~[[Category: Yersiniabactin~~]]	+

OCA at 07:47, 27 November 2019

2019-11-27T07:47:28Z

←Older revision		Revision as of 07:47, 27 November 2019
Line 1:		Line 1:

	==Substrate Analog and NADP+ bound structure of Irp3, a Thiazolinyl Imine Reductase from Yersinia enterocolitica==		==Substrate Analog and NADP+ bound structure of Irp3, a Thiazolinyl Imine Reductase from Yersinia enterocolitica==
-	<StructureSection load='5kvs' size='340' side='right' caption='[[5kvs]], [[Resolution\|resolution]] 1.28Å' scene=''>	+	<StructureSection load='5kvs' size='340' side='right'caption='[[5kvs]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5kvs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KVS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5kvs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_enterocoliticum"_schleifstein_and_coleman_1939 "bacterium enterocoliticum" schleifstein and coleman 1939]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KVS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6XR:(4~{R})-2-[2-(2-HYDROXYPHENYL)-1,3-THIAZOL-4-YL]-4,5-DIHYDRO-1,3-THIAZOLE-4-CARBOXYLIC+ACID'>6XR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6XR:(4~{R})-2-[2-(2-HYDROXYPHENYL)-1,3-THIAZOL-4-YL]-4,5-DIHYDRO-1,3-THIAZOLE-4-CARBOXYLIC+ACID'>6XR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kvq\|5kvq]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kvq\|5kvq]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">irp3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 "Bacterium enterocoliticum" Schleifstein and Coleman 1939])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kvs OCA], [http://pdbe.org/5kvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kvs RCSB], [http://www.ebi.ac.uk/pdbsum/5kvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kvs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kvs OCA], [http://pdbe.org/5kvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kvs RCSB], [http://www.ebi.ac.uk/pdbsum/5kvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kvs ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacterium enterocoliticum schleifstein and coleman 1939]]
		+	[[Category: Large Structures]]
	[[Category: Lamb, A L]]		[[Category: Lamb, A L]]
	[[Category: Meneely, K M]]		[[Category: Meneely, K M]]

OCA at 13:57, 21 September 2016

2016-09-21T13:57:29Z

←Older revision		Revision as of 13:57, 21 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5kvs is ~~ON HOLD until Paper~~ Publication	+	==Substrate Analog and NADP+ bound structure of Irp3, a Thiazolinyl Imine Reductase from Yersinia enterocolitica==
		+	<StructureSection load='5kvs' size='340' side='right' caption='[[5kvs]], [[Resolution\|resolution]] 1.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5kvs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KVS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6XR:(4~{R})-2-[2-(2-HYDROXYPHENYL)-1,3-THIAZOL-4-YL]-4,5-DIHYDRO-1,3-THIAZOLE-4-CARBOXYLIC+ACID'>6XR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kvq\|5kvq]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kvs OCA], [http://pdbe.org/5kvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kvs RCSB], [http://www.ebi.ac.uk/pdbsum/5kvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kvs ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thiazolinyl imine reductases catalyze the NADPH-dependent reduction of a thiazoline to a thiazolidine, a required step in the formation of the siderophores yersiniabactin (Yersinia spp.) and pyochelin (Pseudomonas aeruginosa). These stand-alone nonribosomal peptide tailoring domains are structural homologues of sugar oxidoreductases. Two closed structures of the thiazolinyl imine reductase from Yersinia enterocolitica (Irp3) are presented here: an NADP+-bound structure to 1.45 A resolution and a holo structure to 1.28 A resolution with NADP+ and a substrate analogue bound. Michaelis-Menten kinetics were measured using the same substrate analogue and the homologue from P. aeruginosa, PchG. The data presented here support the hypothesis that tyrosine 128 is the likely general acid residue for catalysis and also highlight the phosphopantetheine tunnel for tethering of the substrate to the nonribosomal peptide synthetase module during assembly line biosynthesis of the siderophore.

-	~~Authors:~~ Meneely, ~~K.M.~~, ~~Lamb~~, A.L.	+	Holo Structure and Steady State Kinetics of the Thiazolinyl Imine Reductases for Siderophore Biosynthesis.,Meneely KM, Ronnebaum TA, Riley AP, Prisinzano TE, Lamb AL Biochemistry. 2016 Sep 15. PMID:27601130<ref>PMID:27601130</ref>

-	~~Description: Substrate Analog and NADP+ bound structure of Irp3~~, a ~~Thiazolinyl Imine Reductase from Yersinia enterocolitica~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Lamb, A.L]]	+	<div class="pdbe-citations 5kvs" style="background-color:#fffaf0;"></div>
-	[[Category: Meneely, K.M]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Lamb, A L]]
		+	[[Category: Meneely, K M]]
		+	[[Category: Hptt-cooh]]
		+	[[Category: Imine reductase]]
		+	[[Category: Irp3]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Siderophore]]
		+	[[Category: Substrate analog]]
		+	[[Category: Yersiniabactin]]

OCA at 13:48, 10 August 2016

2016-08-10T13:48:39Z

←Older revision		Revision as of 13:48, 10 August 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5kvs is ON HOLD	+	The entry 5kvs is ON HOLD until Paper Publication

	Authors: Meneely, K.M., Lamb, A.L.		Authors: Meneely, K.M., Lamb, A.L.

OCA: Protected "5kvs" [edit=sysop:move=sysop]

2016-07-27T13:25:16Z

Protected "5kvs" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:25, 27 July 2016

OCA: New page: '''Unreleased structure''' The entry 5kvs is ON HOLD Authors: Meneely, K.M., Lamb, A.L. Description: Substrate Analog and NADP+ bound structure of Irp3, a Thiazolinyl Imine Reductase f...

2016-07-27T13:25:15Z

New page: '''Unreleased structure''' The entry 5kvs is ON HOLD Authors: Meneely, K.M., Lamb, A.L. Description: Substrate Analog and NADP+ bound structure of Irp3, a Thiazolinyl Imine Reductase f...

New page

'''Unreleased structure'''

The entry 5kvs is ON HOLD

Authors: Meneely, K.M., Lamb, A.L.

Description: Substrate Analog and NADP+ bound structure of Irp3, a Thiazolinyl Imine Reductase from Yersinia enterocolitica
[[Category: Unreleased Structures]]
[[Category: Lamb, A.L]]
[[Category: Meneely, K.M]]