5l0x - Revision history

OCA: Redirecting to 6d7r

2022-04-27T11:35:40Z

Redirecting to 6d7r

←Older revision		Revision as of 11:35, 27 April 2022
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-		+	#REDIRECT [[6d7r]] This PDB entry is obsolete and replaced by 6d7r
-	~~==Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D==~~	+
-	~~<StructureSection load='5l0x' size='340' side='right'caption='~~[[~~5l0x]], [[Resolution\|resolution]] 2.10Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[5l0x~~]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L0X FirstGlance]. <br>	+
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hapR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0x OCA], [http://pdbe.org/5l0x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l0x RCSB], [http://www.ebi.ac.uk/pdbsum/5l0x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0x ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~HapR is a TetR-family transcriptional regulator that controls quorum sensing in Vibrio cholerae, the causative agent of cholera. HapR regulates the expression of hemagglutinin protease, virulence~~ and biofilm genes. The crystal structure of wild-type HapR from V. cholerae strain O1 El Tor C6706 has previously been solved. In this study, the structure of a DNA-binding-deficient variant of HapR (HapRV2) derived from the protease-deficient V. cholerae serotype O37 strain V2 is reported. The structure reveals no structural differences compared with wild-type HapR. However, structural alignment of HapRV2 with the TetR-family member QacR in complex with its operator DNA suggests that the aspartate residue located between the regulatory and DNA-binding domains may clash with and electrostatically repel the phosphate backbone of DNA to prevent binding.	+
-		+
-	Crystal structure of an inactive variant of the quorum-sensing master regulator HapR from the protease-deficient non-O1, non-O139 Vibrio cholerae strain V2.,Cruite J, Succo P, Raychaudhuri S, Kull FJ Acta Crystallogr F Struct Biol Commun. 2018 Jun 1;74(Pt 6):331-336. doi:, 10.1107/S2053230X18006519. Epub 2018 May 17. PMID:29870016<ref>PMID:29870016</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5l0x" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Bacillo virgola del koch trevisan 1884]]~~	+
-	~~[[Category: Large Structures]]~~	+
-	~~[[Category: Cruite, J]]~~	+
-	~~[[Category: Kull, F J]]~~	+
-	~~[[Category: Hapr]]~~	+
-	~~[[Category: Quorum]]~~	+
-	~~[[Category: Tetr]]~~	+
-	~~[[Category: Transcription]]~~	+

OCA at 16:30, 11 December 2019

2019-12-11T16:30:59Z

←Older revision		Revision as of 16:30, 11 December 2019
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	==Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D==		==Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D==
-	<StructureSection load='5l0x' size='340' side='right' caption='[[5l0x]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5l0x' size='340' side='right'caption='[[5l0x]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5l0x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L0X FirstGlance]. <br>		<table><tr><td colspan='2'>[[5l0x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L0X FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillo virgola del koch trevisan 1884]]		[[Category: Bacillo virgola del koch trevisan 1884]]
		+	[[Category: Large Structures]]
	[[Category: Cruite, J]]		[[Category: Cruite, J]]
	[[Category: Kull, F J]]		[[Category: Kull, F J]]

OCA at 09:40, 13 February 2019

2019-02-13T09:40:20Z

←Older revision		Revision as of 09:40, 13 February 2019
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0x OCA], [http://pdbe.org/5l0x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l0x RCSB], [http://www.ebi.ac.uk/pdbsum/5l0x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0x OCA], [http://pdbe.org/5l0x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l0x RCSB], [http://www.ebi.ac.uk/pdbsum/5l0x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0x ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	HapR is a TetR-family transcriptional regulator that controls quorum sensing in Vibrio cholerae, the causative agent of cholera. HapR regulates the expression of hemagglutinin protease, virulence and biofilm genes. The crystal structure of wild-type HapR from V. cholerae strain O1 El Tor C6706 has previously been solved. In this study, the structure of a DNA-binding-deficient variant of HapR (HapRV2) derived from the protease-deficient V. cholerae serotype O37 strain V2 is reported. The structure reveals no structural differences compared with wild-type HapR. However, structural alignment of HapRV2 with the TetR-family member QacR in complex with its operator DNA suggests that the aspartate residue located between the regulatory and DNA-binding domains may clash with and electrostatically repel the phosphate backbone of DNA to prevent binding.
		+
		+	Crystal structure of an inactive variant of the quorum-sensing master regulator HapR from the protease-deficient non-O1, non-O139 Vibrio cholerae strain V2.,Cruite J, Succo P, Raychaudhuri S, Kull FJ Acta Crystallogr F Struct Biol Commun. 2018 Jun 1;74(Pt 6):331-336. doi:, 10.1107/S2053230X18006519. Epub 2018 May 17. PMID:29870016<ref>PMID:29870016</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5l0x" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:57, 13 November 2017

2017-11-13T07:57:19Z

←Older revision		Revision as of 07:57, 13 November 2017
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-	'''Unreleased structure'''

-	~~The entry 5l0x is ON HOLD until Paper Publication~~	+	==Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D==
-		+	<StructureSection load='5l0x' size='340' side='right' caption='[[5l0x]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors: Cruite, J., Kull, F.J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5l0x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L0X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L0X FirstGlance]. <br>
-	~~Description:~~ Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hapR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l0x OCA], [http://pdbe.org/5l0x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l0x RCSB], [http://www.ebi.ac.uk/pdbsum/5l0x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l0x ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Kull~~, F.J]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Cruite, J]]		[[Category: Cruite, J]]
		+	[[Category: Kull, F J]]
		+	[[Category: Hapr]]
		+	[[Category: Quorum]]
		+	[[Category: Tetr]]
		+	[[Category: Transcription]]

OCA at 14:30, 9 September 2016

2016-09-09T14:30:05Z

←Older revision		Revision as of 14:30, 9 September 2016
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5l0x is ON HOLD	+	The entry 5l0x is ON HOLD until Paper Publication

	Authors: Cruite, J., Kull, F.J.		Authors: Cruite, J., Kull, F.J.

OCA at 13:58, 10 August 2016

2016-08-10T13:58:09Z

←Older revision		Revision as of 13:58, 10 August 2016
Line 3:		Line 3:
	The entry 5l0x is ON HOLD		The entry 5l0x is ON HOLD

-	Authors: Cruite, ~~Justin~~, Kull, F. ~~Jon~~	+	Authors: Cruite, J., Kull, F.J.

	Description: Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D		Description: Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Cruite, Justin,~~ Kull, F. ~~Jon~~]]	+	[[Category: Kull, F.J]]
		+	[[Category: Cruite, J]]

OCA: Protected "5l0x" [edit=sysop:move=sysop]

2016-08-04T02:21:01Z

Protected "5l0x" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:21, 4 August 2016

OCA: New page: '''Unreleased structure''' The entry 5l0x is ON HOLD Authors: Cruite, Justin, Kull, F. Jon Description: Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D [[C...

2016-08-04T02:20:59Z

New page: '''Unreleased structure''' The entry 5l0x is ON HOLD Authors: Cruite, Justin, Kull, F. Jon Description: Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D [[C...

New page

'''Unreleased structure'''

The entry 5l0x is ON HOLD

Authors: Cruite, Justin, Kull, F. Jon

Description: Crystal structure of inactive V. cholerae quorum-sensing regulator HapR G39D
[[Category: Unreleased Structures]]
[[Category: Cruite, Justin, Kull, F. Jon]]