5l95 - Revision history

OCA at 16:11, 4 October 2023

2023-10-04T16:11:51Z

←Older revision		Revision as of 16:11, 4 October 2023
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	<StructureSection load='5l95' size='340' side='right'caption='[[5l95]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='5l95' size='340' side='right'caption='[[5l95]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5l95]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L95 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5L95 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5l95]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L95 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~UBA5, UBE1DC1 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN]), UFM1, C13orf20, BM-002 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5l95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l95 OCA], [~~http~~://pdbe.org/5l95 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5l95 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5l95 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5l95 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l95 OCA], [https://pdbe.org/5l95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l95 RCSB], [https://www.ebi.ac.uk/pdbsum/5l95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l95 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UBA5_HUMAN UBA5_HUMAN]] E1-like enzyme which activates UFM1 and SUMO2.<ref>PMID:15071506</ref> <ref>PMID:18442052</ref> <ref>PMID:20368332</ref> ~~[[http://www.uniprot.org/uniprot/UFM1_HUMAN UFM1_HUMAN]] Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.<ref>PMID:15071506</ref> <ref>PMID:20018847</ref>~~	+	[https://www.uniprot.org/uniprot/UBA5_HUMAN UBA5_HUMAN] E1-like enzyme which activates UFM1 and SUMO2.<ref>PMID:15071506</ref> <ref>PMID:18442052</ref> <ref>PMID:20368332</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Ubiquitin activating enzyme\|Ubiquitin activating enzyme]]	+	*[[Ubiquitin-fold modifier\|Ubiquitin-fold modifier]]
		+	*[[3D structures of Ubiquitin activating enzyme\|3D structures of Ubiquitin activating enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Oweis, W]]	+	[[Category: Oweis W]]
-	[[Category: Padala, P]]	+	[[Category: Padala P]]
-	[[Category: Wiener, R]]	+	[[Category: Wiener R]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Ubiquitin like protein e1]]~~	+
-	~~[[Category: Ubl~~]]	+

OCA at 07:02, 5 August 2020

2020-08-05T07:02:41Z

←Older revision		Revision as of 07:02, 5 August 2020
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	==Crystal structure of human UBA5 in complex with UFM1 and AMP==		==Crystal structure of human UBA5 in complex with UFM1 and AMP==
-	<StructureSection load='5l95' size='340' side='right' caption='[[5l95]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='5l95' size='340' side='right'caption='[[5l95]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5l95]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L95 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5L95 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5l95]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L95 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5L95 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5l95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l95 OCA], [http://pdbe.org/5l95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l95 RCSB], [http://www.ebi.ac.uk/pdbsum/5l95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l95 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UBA5, UBE1DC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), UFM1, C13orf20, BM-002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5l95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l95 OCA], [http://pdbe.org/5l95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l95 RCSB], [http://www.ebi.ac.uk/pdbsum/5l95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l95 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5l95" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5l95" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ubiquitin activating enzyme\|Ubiquitin activating enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Oweis, W]]		[[Category: Oweis, W]]
	[[Category: Padala, P]]		[[Category: Padala, P]]

OCA at 14:27, 5 October 2016

2016-10-05T14:27:29Z

←Older revision		Revision as of 14:27, 5 October 2016
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/UBA5_HUMAN UBA5_HUMAN]] E1-like enzyme which activates UFM1 and SUMO2.<ref>PMID:15071506</ref> <ref>PMID:18442052</ref> <ref>PMID:20368332</ref> [[http://www.uniprot.org/uniprot/UFM1_HUMAN UFM1_HUMAN]] Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.<ref>PMID:15071506</ref> <ref>PMID:20018847</ref>		[[http://www.uniprot.org/uniprot/UBA5_HUMAN UBA5_HUMAN]] E1-like enzyme which activates UFM1 and SUMO2.<ref>PMID:15071506</ref> <ref>PMID:18442052</ref> <ref>PMID:20368332</ref> [[http://www.uniprot.org/uniprot/UFM1_HUMAN UFM1_HUMAN]] Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.<ref>PMID:15071506</ref> <ref>PMID:20018847</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Modification of proteins by ubiquitin or ubiquitin-like proteins (UBLs) is a critical cellular process implicated in a variety of cellular states and outcomes. A prerequisite for target protein modification by a UBL is the activation of the latter by activating enzymes (E1s). Here, we present the crystal structure of the non-canonical homodimeric E1, UBA5, in complex with its cognate UBL, UFM1, and supporting biochemical experiments. We find that UBA5 binds to UFM1 via a trans-binding mechanism in which UFM1 interacts with distinct sites in both subunits of the UBA5 dimer. This binding mechanism requires a region C-terminal to the adenylation domain that brings UFM1 to the active site of the adjacent UBA5 subunit. We also find that transfer of UFM1 from UBA5 to the E2, UFC1, occurs via a trans mechanism, thereby requiring a homodimer of UBA5. These findings explicitly elucidate the role of UBA5 dimerization in UFM1 activation.
		+
		+	Trans-Binding Mechanism of Ubiquitin-like Protein Activation Revealed by a UBA5-UFM1 Complex.,Oweis W, Padala P, Hassouna F, Cohen-Kfir E, Gibbs DR, Todd EA, Berndsen CE, Wiener R Cell Rep. 2016 Sep 20;16(12):3113-20. doi: 10.1016/j.celrep.2016.08.067. PMID:27653677<ref>PMID:27653677</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5l95" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:58, 21 September 2016

2016-09-21T13:58:36Z

←Older revision		Revision as of 13:58, 21 September 2016
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-	'''Unreleased structure'''

-	~~The entry~~ 5l95 is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Crystal structure of human UBA5 in complex with UFM1 and AMP==
-		+	<StructureSection load='5l95' size='340' side='right' caption='[[5l95]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5l95]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L95 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5L95 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5l95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l95 OCA], [http://pdbe.org/5l95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5l95 RCSB], [http://www.ebi.ac.uk/pdbsum/5l95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5l95 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UBA5_HUMAN UBA5_HUMAN]] E1-like enzyme which activates UFM1 and SUMO2.<ref>PMID:15071506</ref> <ref>PMID:18442052</ref> <ref>PMID:20368332</ref> [[http://www.uniprot.org/uniprot/UFM1_HUMAN UFM1_HUMAN]] Ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.<ref>PMID:15071506</ref> <ref>PMID:20018847</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Oweis, W]]
		+	[[Category: Padala, P]]
		+	[[Category: Wiener, R]]
		+	[[Category: Cell cycle]]
		+	[[Category: Ubiquitin like protein e1]]
		+	[[Category: Ubl]]

OCA at 14:41, 9 September 2016

2016-09-09T14:41:58Z

←Older revision		Revision as of 14:41, 9 September 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5l95 is ON HOLD	+	The entry 5l95 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5l95" [edit=sysop:move=sysop]

2016-06-20T14:04:43Z

Protected "5l95" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:04, 20 June 2016

OCA: New page: '''Unreleased structure''' The entry 5l95 is ON HOLD Authors: Description: Category: Unreleased Structures

2016-06-20T14:04:42Z

New page: '''Unreleased structure''' The entry 5l95 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5l95 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]