5lmz - Revision history

OCA at 18:35, 18 October 2023

2023-10-18T18:35:25Z

←Older revision		Revision as of 18:35, 18 October 2023
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	<StructureSection load='5lmz' size='340' side='right'caption='[[5lmz]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='5lmz' size='340' side='right'caption='[[5lmz]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lmz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Streptomyces_sp.~~_ma37~~ Streptomyces sp. ~~ma37~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LMZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5LMZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lmz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._MA37 Streptomyces sp. MA37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LMZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1DA:1-DEAZA-ADENOSINE'>1DA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1400207 Streptomyces sp. MA37])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1DA:1-DEAZA-ADENOSINE'>1DA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5lmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lmz OCA], [~~http~~://pdbe.org/5lmz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lmz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lmz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lmz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lmz OCA], [https://pdbe.org/5lmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lmz RCSB], [https://www.ebi.ac.uk/pdbsum/5lmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lmz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/W0W999_9ACTN W0W999_9ACTN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Streptomyces sp. ~~ma37~~]]	+	[[Category: Streptomyces sp. MA37]]
-	[[Category: Bandaranayaka, N]]	+	[[Category: Bandaranayaka N]]
-	~~[[Category: Hagan, D O~~]]	+	[[Category: Mann G]]
-	[[Category: Mann, G]]	+	[[Category: Naismith JH]]
-	[[Category: Naismith~~, J H~~]]	+	[[Category: O'Hagan D]]
-	[[Category: ~~Fluorinase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:42, 12 August 2020

2020-08-12T10:42:46Z

←Older revision		Revision as of 10:42, 12 August 2020
Line 1:		Line 1:

	==Fluorinase from Streptomyces sp. MA37==		==Fluorinase from Streptomyces sp. MA37==
-	<StructureSection load='5lmz' size='340' side='right' caption='[[5lmz]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='5lmz' size='340' side='right'caption='[[5lmz]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lmz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LMZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LMZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lmz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp._ma37 Streptomyces sp. ma37]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LMZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5LMZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1DA:1-DEAZA-ADENOSINE'>1DA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1DA:1-DEAZA-ADENOSINE'>1DA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lmz OCA], [http://pdbe.org/5lmz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lmz RCSB], [http://www.ebi.ac.uk/pdbsum/5lmz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lmz ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1400207 Streptomyces sp. MA37])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5lmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lmz OCA], [http://pdbe.org/5lmz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lmz RCSB], [http://www.ebi.ac.uk/pdbsum/5lmz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lmz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Streptomyces sp. ma37]]
	[[Category: Bandaranayaka, N]]		[[Category: Bandaranayaka, N]]
	[[Category: Hagan, D O]]		[[Category: Hagan, D O]]

OCA at 06:55, 10 September 2016

2016-09-10T06:55:27Z

←Older revision		Revision as of 06:55, 10 September 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5lmz is ~~ON HOLD~~	+	==Fluorinase from Streptomyces sp. MA37==
		+	<StructureSection load='5lmz' size='340' side='right' caption='[[5lmz]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5lmz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LMZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LMZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1DA:1-DEAZA-ADENOSINE'>1DA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lmz OCA], [http://pdbe.org/5lmz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lmz RCSB], [http://www.ebi.ac.uk/pdbsum/5lmz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lmz ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The fluorinase is an enzyme that catalyses the combination of S-adenosyl-L-methionine (SAM) and a fluoride ion to generate 5'-fluorodeoxy adenosine (FDA) and L-methionine through a nucleophilic substitution reaction with a fluoride ion as the nucleophile. It is the only native fluorination enzyme that has been characterised. The fluorinase was isolated in 2002 from Streptomyces cattleya, and, to date, this has been the only source of the fluorinase enzyme. Herein, we report three new fluorinase isolates that have been identified by genome mining. The novel fluorinases from Streptomyces sp. MA37, Nocardia brasiliensis, and an Actinoplanes sp. have high homology (80-87 % identity) to the original S. cattleya enzyme. They all possess a characteristic 21-residue loop. The three newly identified genes were overexpressed in E. coli and shown to be fluorination enzymes. An X-ray crystallographic study of the Streptomyces sp. MA37 enzyme demonstrated that it is almost identical in structure to the original fluorinase. Culturing of the Streptomyces sp. MA37 strain demonstrated that it not only also elaborates the fluorometabolites, fluoroacetate and 4-fluorothreonine, similar to S. cattleya, but this strain also produces a range of unidentified fluorometabolites. These are the first new fluorinases to be reported since the first isolate, over a decade ago, and their identification extends the range of fluorination genes available for fluorination biotechnology.

-	~~Authors: Mann~~, G., O'Hagan, D.~~, Naismith, J~~.H., ~~Bandaranayaka, N~~.	+	Identification of fluorinases from Streptomyces sp MA37, Norcardia brasiliensis, and Actinoplanes sp N902-109 by genome mining.,Deng H, Ma L, Bandaranayaka N, Qin Z, Mann G, Kyeremeh K, Yu Y, Shepherd T, Naismith JH, O'Hagan D Chembiochem. 2014 Feb 10;15(3):364-8. doi: 10.1002/cbic.201300732. Epub 2014 Jan , 21. PMID:24449539<ref>PMID:24449539</ref>

-	~~Description: Fluorinase from Streptomyces sp~~. ~~MA37~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Naismith, J.H]]~~	+	<div class="pdbe-citations 5lmz" style="background-color:#fffaf0;"></div>
-	~~[[Category: O'Hagan, D]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bandaranayaka, N]]		[[Category: Bandaranayaka, N]]
		+	[[Category: Hagan, D O]]
	[[Category: Mann, G]]		[[Category: Mann, G]]
		+	[[Category: Naismith, J H]]
		+	[[Category: Fluorinase]]
		+	[[Category: Transferase]]

OCA: Protected "5lmz" [edit=sysop:move=sysop]

2016-08-10T14:40:29Z

Protected "5lmz" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:40, 10 August 2016

OCA: New page: '''Unreleased structure''' The entry 5lmz is ON HOLD Authors: Mann, G., O'Hagan, D., Naismith, J.H., Bandaranayaka, N. Description: Fluorinase from Streptomyces sp. MA37 [[Category: Un...

2016-08-10T14:40:28Z

New page: '''Unreleased structure''' The entry 5lmz is ON HOLD Authors: Mann, G., O'Hagan, D., Naismith, J.H., Bandaranayaka, N. Description: Fluorinase from Streptomyces sp. MA37 [[Category: Un...

New page

'''Unreleased structure'''

The entry 5lmz is ON HOLD

Authors: Mann, G., O'Hagan, D., Naismith, J.H., Bandaranayaka, N.

Description: Fluorinase from Streptomyces sp. MA37
[[Category: Unreleased Structures]]
[[Category: Naismith, J.H]]
[[Category: O'Hagan, D]]
[[Category: Bandaranayaka, N]]
[[Category: Mann, G]]