5lo8 - Revision history

OCA at 18:37, 18 October 2023

2023-10-18T18:37:19Z

←Older revision		Revision as of 18:37, 18 October 2023
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	==The C2B domain of Rabphilin 3A in complex with PI(4,5)P2==		==The C2B domain of Rabphilin 3A in complex with PI(4,5)P2==
-	<StructureSection load='5lo8' size='340' side='right' caption='[[5lo8]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='5lo8' size='340' side='right'caption='[[5lo8]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5lo8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LO8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5LO8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5lo8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LO8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rph3a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5lo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lo8 OCA], [~~http~~://pdbe.org/5lo8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5lo8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5lo8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5lo8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lo8 OCA], [https://pdbe.org/5lo8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lo8 RCSB], [https://www.ebi.ac.uk/pdbsum/5lo8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lo8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RP3A_RAT RP3A_RAT]] Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.	+	[https://www.uniprot.org/uniprot/RP3A_RAT RP3A_RAT] Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5lo8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5lo8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Exophilin 3D structures\|Exophilin 3D structures]]
		+	*[[Rabphilin\|Rabphilin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Buffalo rat~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Ferrer-Orta, C~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Verdaguer, N~~]]	+	[[Category: Ferrer-Orta C]]
-	[[Category: ~~C2 domain]]~~	+	[[Category: Verdaguer N]]
-	~~[[Category: Pip2]]~~	+
-	~~[[Category: Protein transport]]~~	+
-	~~[[Category: Vesicle fusion~~]]	+

OCA at 07:39, 6 December 2017

2017-12-06T07:39:50Z

←Older revision		Revision as of 07:39, 6 December 2017
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/RP3A_RAT RP3A_RAT]] Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.		[[http://www.uniprot.org/uniprot/RP3A_RAT RP3A_RAT]] Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Membrane fusion is essential in a myriad of eukaryotic cell biological processes, including the synaptic transmission. Rabphilin-3A is a membrane trafficking protein involved in the calcium-dependent regulation of secretory vesicle exocytosis in neurons and neuroendocrine cells, but the underlying mechanism remains poorly understood. Here, we report the crystal structures and biochemical analyses of Rabphilin-3A C2B-SNAP25 and C2B-phosphatidylinositol 4,5-bisphosphate (PIP2) complexes, revealing how Rabphilin-3A C2 domains operate in cooperation with PIP2/Ca(2+) and SNAP25 to bind the plasma membrane, adopting a conformation compatible to interact with the complete SNARE complex. Comparisons with the synaptotagmin1-SNARE show that both proteins contact the same SNAP25 surface, but Rabphilin-3A uses a unique structural element. Data obtained here suggest a model to explain the Ca(2+)-dependent fusion process by membrane bending with a myriad of variations depending on the properties of the C2 domain-bearing protein, shedding light to understand the fine-tuning control of the different vesicle fusion events.
		+
		+	Structural characterization of the Rabphilin-3A-SNAP25 interaction.,Ferrer-Orta C, Perez-Sanchez MD, Coronado-Parra T, Silva C, Lopez-Martinez D, Baltanas-Copado J, Gomez-Fernandez JC, Corbalan-Garcia S, Verdaguer N Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):E5343-E5351. doi:, 10.1073/pnas.1702542114. Epub 2017 Jun 20. PMID:28634303<ref>PMID:28634303</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5lo8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:42, 16 November 2017

2017-11-16T08:42:44Z

←Older revision		Revision as of 08:42, 16 November 2017
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-	'''Unreleased structure'''

-	The ~~entry~~ 5lo8 is ~~ON HOLD until Paper Publication~~	+	==The C2B domain of Rabphilin 3A in complex with PI(4,5)P2==
-		+	<StructureSection load='5lo8' size='340' side='right' caption='[[5lo8]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5lo8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LO8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LO8 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PIO:[(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2,3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY-PHOSPHORYL]OXY-PROPYL]+OCTANOATE'>PIO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rph3a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lo8 OCA], [http://pdbe.org/5lo8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lo8 RCSB], [http://www.ebi.ac.uk/pdbsum/5lo8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lo8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RP3A_RAT RP3A_RAT]] Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Buffalo rat]]
		+	[[Category: Ferrer-Orta, C]]
		+	[[Category: Verdaguer, N]]
		+	[[Category: C2 domain]]
		+	[[Category: Pip2]]
		+	[[Category: Protein transport]]
		+	[[Category: Vesicle fusion]]

OCA at 19:03, 5 October 2016

2016-10-05T19:03:41Z

←Older revision		Revision as of 19:03, 5 October 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5lo8 is ON HOLD	+	The entry 5lo8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5lo8" [edit=sysop:move=sysop]

2016-09-09T15:05:46Z

Protected "5lo8" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:05, 9 September 2016

OCA: New page: '''Unreleased structure''' The entry 5lo8 is ON HOLD Authors: Description: Category: Unreleased Structures

2016-09-09T15:05:45Z

New page: '''Unreleased structure''' The entry 5lo8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5lo8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]