5m09 - Revision history

OCA at 18:19, 1 November 2023

2023-11-01T18:19:04Z

←Older revision		Revision as of 18:19, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='5m09' size='340' side='right'caption='[[5m09]], [[Resolution\|resolution]] 2.98Å' scene=''>		<StructureSection load='5m09' size='340' side='right'caption='[[5m09]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m09]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M09 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5M09 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m09]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M09 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M09 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.98Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>pknI, Rv2914c, MTCY338.02c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83332 MYCTU])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m09 OCA], [https://pdbe.org/5m09 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m09 RCSB], [https://www.ebi.ac.uk/pdbsum/5m09 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m09 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5m09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m09 OCA], [~~http~~://pdbe.org/5m09 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5m09 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5m09 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5m09 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PKNI_MYCTU PKNI_MYCTU]] Plays an important role in slowing down the growth of mycobacteria within the infected host.<ref>PMID:19341393</ref>	+	[https://www.uniprot.org/uniprot/PKNI_MYCTU PKNI_MYCTU] Plays an important role in slowing down the growth of mycobacteria within the infected host.<ref>PMID:19341393</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 28:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu]]~~	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	~~[[Category: Non-specific serine/threonine protein kinase~~]]	+	[[Category: Alexandre M]]
-	[[Category: Alexandre, M]]	+	[[Category: Alzari PM]]
-	[[Category: Alzari~~, P M~~]]	+	[[Category: Barilone N]]
-	[[Category: Barilone, N]]	+	[[Category: Bellinzoni M]]
-	[[Category: Bellinzoni, M]]	+	[[Category: Lisa MN]]
-	[[Category: Lisa~~, M N~~]]	+	[[Category: Raynal B]]
-	[[Category: Raynal, B]]	+	[[Category: Wagner T]]
-	[[Category: Wagner, T]]	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Signalling]]~~	+
-	~~[[Category: Tuberculosis~~]]	+

OCA at 10:57, 12 August 2020

2020-08-12T10:57:04Z

←Older revision		Revision as of 10:57, 12 August 2020
Line 1:		Line 1:

	==Crystal structure of Mycobacterium tuberculosis PknI kinase domain, C20A_R136N double mutant==		==Crystal structure of Mycobacterium tuberculosis PknI kinase domain, C20A_R136N double mutant==
-	<StructureSection load='5m09' size='340' side='right' caption='[[5m09]], [[Resolution\|resolution]] 2.98Å' scene=''>	+	<StructureSection load='5m09' size='340' side='right'caption='[[5m09]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m09]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M09 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=5M09 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m09]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M09 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5M09 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pknI, Rv2914c, MTCY338.02c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5m09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m09 OCA], [http://pdbe.org/5m09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m09 RCSB], [http://www.ebi.ac.uk/pdbsum/5m09 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m09 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5m09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m09 OCA], [http://pdbe.org/5m09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m09 RCSB], [http://www.ebi.ac.uk/pdbsum/5m09 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m09 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5m09" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5m09" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Serine/threonine protein kinase 3D structures\|Serine/threonine protein kinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Non-specific serine/threonine protein kinase]]		[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Alexandre, M]]		[[Category: Alexandre, M]]

OCA at 08:36, 18 January 2017

2017-01-18T08:36:20Z

←Older revision		Revision as of 08:36, 18 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5m09 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of Mycobacterium tuberculosis PknI kinase domain, C20A_R136N double mutant==
		+	<StructureSection load='5m09' size='340' side='right' caption='[[5m09]], [[Resolution\|resolution]] 2.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5m09]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M09 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M09 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m09 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m09 OCA], [http://pdbe.org/5m09 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m09 RCSB], [http://www.ebi.ac.uk/pdbsum/5m09 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m09 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PKNI_MYCTU PKNI_MYCTU]] Plays an important role in slowing down the growth of mycobacteria within the infected host.<ref>PMID:19341393</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Eukaryotic-like Ser/Thr protein kinases (ePKs) have been identified in many bacterial species, where they are known to mediate signalling mechanisms that share several features with their eukaryotic counterparts. In Mycobacterium tuberculosis, PknI is one of the eleven predicted ePKs and it has been related to bacterial virulence. In order to better understand the molecular basis of its role in mycobacterial signalling, we solved the crystal structure of the PknI cytoplasmic domain. We found that even though PknI possesses most conserved elements characteristic of Hanks-type kinases, it is degraded in several motifs that are essential for the ePKs catalytic activity. Most notably, PknI presents a remarkably short activation segment lacking a peptide-substrate binding site. Consistently with this observation and similarly to earlier findings for eukaryotic pseudokinases, no kinase activity was detected for the catalytic domain of PknI, against different substrates and in various experimental conditions. Based on these results we conclude that PknI may rely on unconventional mechanism(s) for kinase activity and/or it could play alternative role(s) in mycobacterial signalling. This article is protected by copyright. All rights reserved.

-	~~Authors: Lisa~~, ~~M.N~~., Wagner, T., Alexandre, M., Barilone, N., Raynal, B., Alzari~~, P.M.~~, Bellinzoni, M.	+	The crystal structure of PknI from Mycobacterium tuberculosis shows an inactive, pseudokinase-like conformation.,Lisa MN, Wagner T, Alexandre M, Barilone N, Raynal B, Alzari PM, Bellinzoni M FEBS J. 2017 Jan 5. doi: 10.1111/febs.14003. PMID:28054744<ref>PMID:28054744</ref>

-	~~Description: Crystal structure of Mycobacterium tuberculosis PknI kinase domain~~, ~~C20A_R136N double mutant~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5m09" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Non-specific serine/threonine protein kinase]]
	[[Category: Alexandre, M]]		[[Category: Alexandre, M]]
-	~~[[Category: Wagner, T]]~~	+	[[Category: Alzari, P M]]
-	[[Category: Alzari, P~~.M]]~~	+
-	~~[[Category: Lisa,~~ M.N]]	+
	[[Category: Barilone, N]]		[[Category: Barilone, N]]
-	[[Category: Raynal, B]]
	[[Category: Bellinzoni, M]]		[[Category: Bellinzoni, M]]
		+	[[Category: Lisa, M N]]
		+	[[Category: Raynal, B]]
		+	[[Category: Wagner, T]]
		+	[[Category: Kinase]]
		+	[[Category: Signaling protein]]
		+	[[Category: Signalling]]
		+	[[Category: Tuberculosis]]

OCA at 16:15, 9 December 2016

2016-12-09T16:15:55Z

←Older revision		Revision as of 16:15, 9 December 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5m09 is ON HOLD	+	The entry 5m09 is ON HOLD until Paper Publication

	Authors: Lisa, M.N., Wagner, T., Alexandre, M., Barilone, N., Raynal, B., Alzari, P.M., Bellinzoni, M.		Authors: Lisa, M.N., Wagner, T., Alexandre, M., Barilone, N., Raynal, B., Alzari, P.M., Bellinzoni, M.

OCA: Protected "5m09" [edit=sysop:move=sysop]

2016-10-19T15:00:12Z

Protected "5m09" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:00, 19 October 2016

OCA: New page: '''Unreleased structure''' The entry 5m09 is ON HOLD Authors: Lisa, M.N., Wagner, T., Alexandre, M., Barilone, N., Raynal, B., Alzari, P.M., Bellinzoni, M. Description: Crystal structu...

2016-10-19T15:00:11Z

New page: '''Unreleased structure''' The entry 5m09 is ON HOLD Authors: Lisa, M.N., Wagner, T., Alexandre, M., Barilone, N., Raynal, B., Alzari, P.M., Bellinzoni, M. Description: Crystal structu...

New page

'''Unreleased structure'''

The entry 5m09 is ON HOLD

Authors: Lisa, M.N., Wagner, T., Alexandre, M., Barilone, N., Raynal, B., Alzari, P.M., Bellinzoni, M.

Description: Crystal structure of Mycobacterium tuberculosis PknI kinase domain, C20A_R136N double mutant
[[Category: Unreleased Structures]]
[[Category: Alexandre, M]]
[[Category: Wagner, T]]
[[Category: Alzari, P.M]]
[[Category: Lisa, M.N]]
[[Category: Barilone, N]]
[[Category: Raynal, B]]
[[Category: Bellinzoni, M]]