5m8h - Revision history

OCA at 18:30, 1 November 2023

2023-11-01T18:30:11Z

←Older revision		Revision as of 18:30, 1 November 2023
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	==ATP phosphoribosyltransferase (HisZG ATPPRT) from Psychrobacter arcticus==		==ATP phosphoribosyltransferase (HisZG ATPPRT) from Psychrobacter arcticus==
-	<StructureSection load='5m8h' size='340' side='right' caption='[[5m8h]], [[Resolution\|resolution]] 2.34Å' scene=''>	+	<StructureSection load='5m8h' size='340' side='right'caption='[[5m8h]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m8h]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Psya2 Psya2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M8H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5M8H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m8h]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Psychrobacter_arcticus_273-4 Psychrobacter arcticus 273-4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M8H FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CL:CHLORIDE+ION'>CL</scene~~>, ~~<scene name='pdbligand=MPD:(4S)-~~2~~-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.34Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~hisZ, Psyc_0676 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=259536 PSYA2]), hisG, Psyc_1903 ([http~~:~~//www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=259536 PSYA2])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/ATP_phosphoribosyltransferase ATP phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.17 2.4.2.17]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m8h OCA], [https://pdbe.org/5m8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m8h RCSB], [https://www.ebi.ac.uk/pdbsum/5m8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m8h ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5m8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m8h OCA], [~~http~~://pdbe.org/5m8h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5m8h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5m8h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5m8h ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HISZ_PSYA2 HISZ_PSYA2]] Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.[HAMAP-Rule:MF_00125] [[http://www.uniprot.org/uniprot/HIS1_PSYA2 HIS1_PSYA2]] Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.	+	[https://www.uniprot.org/uniprot/HISZ_PSYA2 HISZ_PSYA2] Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.[HAMAP-Rule:MF_00125]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5m8h" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5m8h" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ATP phosphoribosyl transferase 3D structures\|ATP phosphoribosyl transferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~ATP phosphoribosyltransferase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Psya2~~]]	+	[[Category: Psychrobacter arcticus 273-4]]
-	[[Category: Alphey~~, M S~~]]	+	[[Category: Alphey MS]]
-	[[Category: Ge, Y]]	+	[[Category: Ge Y]]
-	[[Category: Naismith~~, J H~~]]	+	[[Category: Naismith JH]]
-	[[Category: Silva~~, R G.da]]~~	+	[[Category: Da Silva RG]]
-	~~[[Category: Atpprt]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Hisgz]]~~	+
-	~~[[Category: Octameric]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 20:39, 24 January 2018

2018-01-24T20:39:58Z

←Older revision		Revision as of 20:39, 24 January 2018
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	<StructureSection load='5m8h' size='340' side='right' caption='[[5m8h]], [[Resolution\|resolution]] 2.34Å' scene=''>		<StructureSection load='5m8h' size='340' side='right' caption='[[5m8h]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5m8h]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M8H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5m8h]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Psya2 Psya2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M8H FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hisZ, Psyc_0676 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=259536 PSYA2]), hisG, Psyc_1903 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=259536 PSYA2])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ATP_phosphoribosyltransferase ATP phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.17 2.4.2.17] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ATP_phosphoribosyltransferase ATP phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.17 2.4.2.17] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m8h OCA], [http://pdbe.org/5m8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m8h RCSB], [http://www.ebi.ac.uk/pdbsum/5m8h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m8h ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m8h OCA], [http://pdbe.org/5m8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m8h RCSB], [http://www.ebi.ac.uk/pdbsum/5m8h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m8h ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: ATP phosphoribosyltransferase]]		[[Category: ATP phosphoribosyltransferase]]
		+	[[Category: Psya2]]
	[[Category: Alphey, M S]]		[[Category: Alphey, M S]]
	[[Category: Ge, Y]]		[[Category: Ge, Y]]

OCA at 03:54, 6 September 2017

2017-09-06T03:54:42Z

←Older revision		Revision as of 03:54, 6 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5m8h is ~~ON HOLD~~	+	==ATP phosphoribosyltransferase (HisZG ATPPRT) from Psychrobacter arcticus==
		+	<StructureSection load='5m8h' size='340' side='right' caption='[[5m8h]], [[Resolution\|resolution]] 2.34Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5m8h]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M8H FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ATP_phosphoribosyltransferase ATP phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.17 2.4.2.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m8h OCA], [http://pdbe.org/5m8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m8h RCSB], [http://www.ebi.ac.uk/pdbsum/5m8h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m8h ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HISZ_PSYA2 HISZ_PSYA2]] Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.[HAMAP-Rule:MF_00125] [[http://www.uniprot.org/uniprot/HIS1_PSYA2 HIS1_PSYA2]] Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Adenosine 5'-triphosphate phosphoribosyltransferase (ATPPRT) catalyzes the first step in histidine biosynthesis, the condensation of ATP and 5-phospho-alpha-d-ribosyl-1-pyrophosphate to generate N1-(5-phospho-beta-d-ribosyl)-ATP and inorganic pyrophosphate. The enzyme is allosterically inhibited by histidine. Two forms of ATPPRT, encoded by the hisG gene, exist in nature, depending on the species. The long form, HisGL, is a single polypeptide chain with catalytic and regulatory domains. The short form, HisGS, lacks a regulatory domain and cannot bind histidine. HisGS instead is found in complex with a regulatory protein, HisZ, constituting the ATPPRT holoenzyme. HisZ triggers HisGS catalytic activity while rendering it sensitive to allosteric inhibition by histidine. Until recently, HisGS was thought to be catalytically inactive without HisZ. Here, recombinant HisGS and HisZ from the psychrophilic bacterium Psychrobacter arcticus were independently overexpressed and purified. The crystal structure of P. arcticus ATPPRT was determined at 2.34 A resolution, revealing an equimolar HisGS-HisZ hetero-octamer. Steady-state kinetics indicate that both the ATPPRT holoenzyme and HisGS are catalytically active. Surprisingly, HisZ confers only a modest 2-4-fold increase in kcat. Reaction profiles for both enzymes cannot be distinguished by 31P nuclear magnetic resonance, indicating that the same reaction is catalyzed. The temperature dependence of kcat shows deviation from Arrhenius behavior at 308 K with the holoenzyme. Interestingly, such deviation is detected only at 313 K with HisGS. Thermal denaturation by CD spectroscopy resulted in Tm's of 312 and 316 K for HisZ and HisGS, respectively, suggesting that HisZ renders the ATPPRT complex more thermolabile. This is the first characterization of a psychrophilic ATPPRT.

-	~~Authors~~:	+	Kinetics and Structure of a Cold-Adapted Hetero-Octameric ATP Phosphoribosyltransferase.,Stroek R, Ge Y, Talbot PD, Glok MK, Bernas KE, Thomson CM, Gould ER, Alphey MS, Liu H, Florence GJ, Naismith JH, da Silva RG Biochemistry. 2017 Feb 7;56(5):793-803. doi: 10.1021/acs.biochem.6b01138. Epub, 2017 Jan 24. PMID:28092443<ref>PMID:28092443</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5m8h" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: ATP phosphoribosyltransferase]]
		+	[[Category: Alphey, M S]]
		+	[[Category: Ge, Y]]
		+	[[Category: Naismith, J H]]
		+	[[Category: Silva, R G.da]]
		+	[[Category: Atpprt]]
		+	[[Category: Complex]]
		+	[[Category: Hisgz]]
		+	[[Category: Octameric]]
		+	[[Category: Transferase]]

OCA: Protected "5m8h" [edit=sysop:move=sysop]

2016-12-09T16:29:26Z

Protected "5m8h" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:29, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5m8h is ON HOLD Authors: Description: Category: Unreleased Structures

2016-12-09T16:29:25Z

New page: '''Unreleased structure''' The entry 5m8h is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5m8h is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]