5mec - Revision history

OCA at 18:40, 1 November 2023

2023-11-01T18:40:45Z

←Older revision		Revision as of 18:40, 1 November 2023
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	==Crystal structure of yeast Cdt1 middle domain (residues 294-433)==		==Crystal structure of yeast Cdt1 middle domain (residues 294-433)==
-	<StructureSection load='5mec' size='340' side='right' caption='[[5mec]], [[Resolution\|resolution]] 2.13Å' scene=''>	+	<StructureSection load='5mec' size='340' side='right'caption='[[5mec]], [[Resolution\|resolution]] 2.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mec]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MEC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MEC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mec]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MEC FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TAH11~~, ~~CDT1, SID2, YJR046W, J1641 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=4932 ATCC 18824])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.13Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mec OCA], [~~http~~://pdbe.org/5mec PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mec RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mec PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mec ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mec OCA], [https://pdbe.org/5mec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mec RCSB], [https://www.ebi.ac.uk/pdbsum/5mec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mec ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CDT1_YEAST CDT1_YEAST]] DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.<ref>PMID:11560884</ref> <ref>PMID:11967159</ref> <ref>PMID:16387651</ref> <ref>PMID:16824194</ref> <ref>PMID:17825064</ref> <ref>PMID:18006685</ref> <ref>PMID:19896182</ref>	+	[https://www.uniprot.org/uniprot/CDT1_YEAST CDT1_YEAST] DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.<ref>PMID:11560884</ref> <ref>PMID:11967159</ref> <ref>PMID:16387651</ref> <ref>PMID:16824194</ref> <ref>PMID:17825064</ref> <ref>PMID:18006685</ref> <ref>PMID:19896182</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 18824~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Cherepanov, P~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Diffley, J F.X~~]]	+	[[Category: Cherepanov P]]
-	[[Category: ~~Frigola, J~~]]	+	[[Category: Diffley JFX]]
-	[[Category: ~~Pye, V E~~]]	+	[[Category: Frigola J]]
-	[[Category: ~~Cdt1]]~~	+	[[Category: Pye VE]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Dna replication]]~~	+
-	~~[[Category: Mcm]]~~	+
-	~~[[Category: Winged helix]]~~	+
-	~~[[Category: Yeast~~]]	+

OCA at 08:08, 16 November 2017

2017-11-16T08:08:32Z

←Older revision		Revision as of 08:08, 16 November 2017
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	<StructureSection load='5mec' size='340' side='right' caption='[[5mec]], [[Resolution\|resolution]] 2.13Å' scene=''>		<StructureSection load='5mec' size='340' side='right' caption='[[5mec]], [[Resolution\|resolution]] 2.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mec]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MEC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mec]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MEC FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mec OCA], [http://pdbe.org/5mec PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mec RCSB], [http://www.ebi.ac.uk/pdbsum/5mec PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mec ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TAH11, CDT1, SID2, YJR046W, J1641 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mec OCA], [http://pdbe.org/5mec PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mec RCSB], [http://www.ebi.ac.uk/pdbsum/5mec PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mec ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CDT1_YEAST CDT1_YEAST]] DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.<ref>PMID:11560884</ref> <ref>PMID:11967159</ref> <ref>PMID:16387651</ref> <ref>PMID:16824194</ref> <ref>PMID:17825064</ref> <ref>PMID:18006685</ref> <ref>PMID:19896182</ref>		[[http://www.uniprot.org/uniprot/CDT1_YEAST CDT1_YEAST]] DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.<ref>PMID:11560884</ref> <ref>PMID:11967159</ref> <ref>PMID:16387651</ref> <ref>PMID:16824194</ref> <ref>PMID:17825064</ref> <ref>PMID:18006685</ref> <ref>PMID:19896182</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ORC, Cdc6 and Cdt1 act together to load hexameric MCM, the motor of the eukaryotic replicative helicase, into double hexamers at replication origins. Here we show that Cdt1 interacts with MCM subunits Mcm2, 4 and 6, which both destabilizes the Mcm2-5 interface and inhibits MCM ATPase activity. Using X-ray crystallography, we show that Cdt1 contains two winged-helix domains in the C-terminal half of the protein and a catalytically inactive dioxygenase-related N-terminal domain, which is important for MCM loading, but not for subsequent replication. We used these structures together with single-particle electron microscopy to generate three-dimensional models of MCM complexes. These show that Cdt1 stabilizes MCM in a left-handed spiral open at the Mcm2-5 gate. We propose that Cdt1 acts as a brace, holding MCM open for DNA entry and bound to ATP until ORC-Cdc6 triggers ATP hydrolysis by MCM, promoting both Cdt1 ejection and MCM ring closure.
		+
		+	Cdt1 stabilizes an open MCM ring for helicase loading.,Frigola J, He J, Kinkelin K, Pye VE, Renault L, Douglas ME, Remus D, Cherepanov P, Costa A, Diffley JFX Nat Commun. 2017 Jun 23;8:15720. doi: 10.1038/ncomms15720. PMID:28643783<ref>PMID:28643783</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5mec" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
	[[Category: Cherepanov, P]]		[[Category: Cherepanov, P]]
	[[Category: Diffley, J F.X]]		[[Category: Diffley, J F.X]]

OCA at 15:48, 17 May 2017

2017-05-17T15:48:11Z

←Older revision		Revision as of 15:48, 17 May 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mec ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of yeast Cdt1 middle domain (residues 294-433)==
-		+	<StructureSection load='5mec' size='340' side='right' caption='[[5mec]], [[Resolution\|resolution]] 2.13Å' scene=''>
-	~~Authors~~: ~~Pye~~, V.E., ~~Frigola~~, J., ~~Diffley, J~~.F.X., ~~Cherepanov, P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5mec]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MEC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MEC FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure~~ of ~~yeast Cdt1 middle domain~~ (~~residues 294-433~~)	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mec OCA], [http://pdbe.org/5mec PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mec RCSB], [http://www.ebi.ac.uk/pdbsum/5mec PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mec ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Pye~~, V.E]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CDT1_YEAST CDT1_YEAST]] DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.<ref>PMID:11560884</ref> <ref>PMID:11967159</ref> <ref>PMID:16387651</ref> <ref>PMID:16824194</ref> <ref>PMID:17825064</ref> <ref>PMID:18006685</ref> <ref>PMID:19896182</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cherepanov, P]]
		+	[[Category: Diffley, J F.X]]
	[[Category: Frigola, J]]		[[Category: Frigola, J]]
-	[[Category: ~~Diffley~~, ~~J.F.X~~]]	+	[[Category: Pye, V E]]
-	[[Category: ~~Cherepanov, P~~]]	+	[[Category: Cdt1]]
		+	[[Category: Cell cycle]]
		+	[[Category: Dna replication]]
		+	[[Category: Mcm]]
		+	[[Category: Winged helix]]
		+	[[Category: Yeast]]

OCA at 11:13, 9 March 2017

2017-03-09T11:13:53Z

←Older revision		Revision as of 11:13, 9 March 2017
Line 9:		Line 9:
	[[Category: Pye, V.E]]		[[Category: Pye, V.E]]
	[[Category: Frigola, J]]		[[Category: Frigola, J]]
-	[[Category: Cherepanov, P]]
	[[Category: Diffley, J.F.X]]		[[Category: Diffley, J.F.X]]
		+	[[Category: Cherepanov, P]]

OCA: Protected "5mec" [edit=sysop:move=sysop]

2016-12-09T16:43:08Z

Protected "5mec" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:43, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5mec is ON HOLD until Paper Publication Authors: Pye, V.E., Frigola, J., Diffley, J.F.X., Cherepanov, P. Description: Crystal structure of yeast Cd...

2016-12-09T16:43:07Z

New page: '''Unreleased structure''' The entry 5mec is ON HOLD until Paper Publication Authors: Pye, V.E., Frigola, J., Diffley, J.F.X., Cherepanov, P. Description: Crystal structure of yeast Cd...

New page

'''Unreleased structure'''

The entry 5mec is ON HOLD until Paper Publication

Authors: Pye, V.E., Frigola, J., Diffley, J.F.X., Cherepanov, P.

Description: Crystal structure of yeast Cdt1 middle domain (residues 294-433)
[[Category: Unreleased Structures]]
[[Category: Pye, V.E]]
[[Category: Frigola, J]]
[[Category: Cherepanov, P]]
[[Category: Diffley, J.F.X]]