5mi0 - Revision history

OCA at 18:44, 1 November 2023

2023-11-01T18:44:09Z

←Older revision		Revision as of 18:44, 1 November 2023
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	<StructureSection load='5mi0' size='340' side='right'caption='[[5mi0]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='5mi0' size='340' side='right'caption='[[5mi0]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mi0]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~] and [~~http~~://en.wikipedia.org/wiki/~~Plafa Plafa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MI0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5MI0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mi0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MI0 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~Rh5 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=5833 PLAFA])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5mi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mi0 OCA], [~~http~~://pdbe.org/5mi0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mi0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mi0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mi0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mi0 OCA], [https://pdbe.org/5mi0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mi0 RCSB], [https://www.ebi.ac.uk/pdbsum/5mi0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mi0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RH5_PLAF7 RH5_PLAF7] Essential for the invasion of host erythrocytes by blood stage merozoites (PubMed:25583518, PubMed:27374406, PubMed:25296023, PubMed:22080952, PubMed:18827878, PubMed:19000690, PubMed:31204103, PubMed:28186186, PubMed:28409866). By binding P113 at the surface of the merozoite and human BSG/basigin on the erythrocyte membrane, leads to the establishment of a tight junction between the merozoite and host erythrocyte membranes (PubMed:25583518, PubMed:27374406, PubMed:25296023, PubMed:22080952, PubMed:28186186). In addition, the interaction with BSG results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed:27374406, PubMed:28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed:28409866).<ref>PMID:18827878</ref> <ref>PMID:19000690</ref> <ref>PMID:22080952</ref> <ref>PMID:25296023</ref> <ref>PMID:25583518</ref> <ref>PMID:27374406</ref> <ref>PMID:28186186</ref> <ref>PMID:28409866</ref> <ref>PMID:31204103</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Plafa~~]]	+	[[Category: Plasmodium falciparum]]
-	[[Category: Barfod, L]]	+	[[Category: Barfod L]]
-	[[Category: Campeotto, I]]	+	[[Category: Campeotto I]]
-	[[Category: Davey, J]]	+	[[Category: Davey J]]
-	[[Category: Draper~~, S J~~]]	+	[[Category: Draper SJ]]
-	[[Category: Fleishman~~, S J~~]]	+	[[Category: Fleishman SJ]]
-	[[Category: Goldenzweig, A]]	+	[[Category: Goldenzweig A]]
-	[[Category: Higgins~~, M K~~]]	+	[[Category: Higgins MK]]
-	[[Category: Marshall~~, J M~~]]	+	[[Category: Marshall JM]]
-	[[Category: Silk~~, S E~~]]	+	[[Category: Silk SE]]
-	[[Category: Wright~~, K E]]~~	+	[[Category: Wright KE]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Malaria immunogen design rosetta pross~~]]	+

OCA at 07:20, 19 August 2020

2020-08-19T07:20:14Z

←Older revision		Revision as of 07:20, 19 August 2020
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	==A thermally stabilised version of Plasmodium falciparum RH5==		==A thermally stabilised version of Plasmodium falciparum RH5==
-	<StructureSection load='5mi0' size='340' side='right' caption='[[5mi0]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='5mi0' size='340' side='right'caption='[[5mi0]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mi0]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MI0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MI0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mi0]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MI0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5MI0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mi0 OCA], [http://pdbe.org/5mi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mi0 RCSB], [http://www.ebi.ac.uk/pdbsum/5mi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mi0 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rh5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5mi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mi0 OCA], [http://pdbe.org/5mi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mi0 RCSB], [http://www.ebi.ac.uk/pdbsum/5mi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mi0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[3D structures ~~of monoclonal antibody~~\|3D structures ~~of monoclonal antibody~~]]	+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lk3 transgenic mice]]
		+	[[Category: Plafa]]
	[[Category: Barfod, L]]		[[Category: Barfod, L]]
	[[Category: Campeotto, I]]		[[Category: Campeotto, I]]

OCA at 06:52, 9 March 2017

2017-03-09T06:52:34Z

←Older revision		Revision as of 06:52, 9 March 2017
Line 15:		Line 15:
	</div>		</div>
	<div class="pdbe-citations 5mi0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5mi0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[3D structures of monoclonal antibody\|3D structures of monoclonal antibody]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:23, 25 January 2017

2017-01-25T16:23:45Z

←Older revision		Revision as of 16:23, 25 January 2017
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mi0 OCA], [http://pdbe.org/5mi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mi0 RCSB], [http://www.ebi.ac.uk/pdbsum/5mi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mi0 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mi0 OCA], [http://pdbe.org/5mi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mi0 RCSB], [http://www.ebi.ac.uk/pdbsum/5mi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mi0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many promising vaccine candidates from pathogenic viruses, bacteria, and parasites are unstable and cannot be produced cheaply for clinical use. For instance, Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) is essential for erythrocyte invasion, is highly conserved among field isolates, and elicits antibodies that neutralize in vitro and protect in an animal model, making it a leading malaria vaccine candidate. However, functional RH5 is only expressible in eukaryotic systems and exhibits moderate temperature tolerance, limiting its usefulness in hot and low-income countries where malaria prevails. Current approaches to immunogen stabilization involve iterative application of rational or semirational design, random mutagenesis, and biochemical characterization. Typically, each round of optimization yields minor improvement in stability, and multiple rounds are required. In contrast, we developed a one-step design strategy using phylogenetic analysis and Rosetta atomistic calculations to design PfRH5 variants with improved packing and surface polarity. To demonstrate the robustness of this approach, we tested three PfRH5 designs, all of which showed improved stability relative to wild type. The best, bearing 18 mutations relative to PfRH5, expressed in a folded form in bacteria at >1 mg of protein per L of culture, and had 10-15 degrees C higher thermal tolerance than wild type, while also retaining ligand binding and immunogenic properties indistinguishable from wild type, proving its value as an immunogen for a future generation of vaccines against the malaria blood stage. We envision that this efficient computational stability design methodology will also be used to enhance the biophysical properties of other recalcitrant vaccine candidates from emerging pathogens.
		+
		+	One-step design of a stable variant of the malaria invasion protein RH5 for use as a vaccine immunogen.,Campeotto I, Goldenzweig A, Davey J, Barfod L, Marshall JM, Silk SE, Wright KE, Draper SJ, Higgins MK, Fleishman SJ Proc Natl Acad Sci U S A. 2017 Jan 17. pii: 201616903. doi:, 10.1073/pnas.1616903114. PMID:28096331<ref>PMID:28096331</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5mi0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:28, 2 January 2017

2017-01-02T16:28:22Z

←Older revision		Revision as of 16:28, 2 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mi0 is ~~ON HOLD until May 27 2018~~	+	==A thermally stabilised version of Plasmodium falciparum RH5==
-		+	<StructureSection load='5mi0' size='340' side='right' caption='[[5mi0]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5mi0]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MI0 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mi0 OCA], [http://pdbe.org/5mi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mi0 RCSB], [http://www.ebi.ac.uk/pdbsum/5mi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mi0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Barfod, L]]
		+	[[Category: Campeotto, I]]
		+	[[Category: Davey, J]]
		+	[[Category: Draper, S J]]
		+	[[Category: Fleishman, S J]]
		+	[[Category: Goldenzweig, A]]
		+	[[Category: Higgins, M K]]
		+	[[Category: Marshall, J M]]
		+	[[Category: Silk, S E]]
		+	[[Category: Wright, K E]]
		+	[[Category: Immune system]]
		+	[[Category: Malaria immunogen design rosetta pross]]

OCA at 16:18, 15 December 2016

2016-12-15T16:18:49Z

←Older revision		Revision as of 16:18, 15 December 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mi0 is ON HOLD until ~~sometime in the future~~	+	The entry 5mi0 is ON HOLD until May 27 2018

	Authors:		Authors:

OCA: Protected "5mi0" [edit=sysop:move=sysop]

2016-12-09T16:53:17Z

Protected "5mi0" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:53, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5mi0 is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

2016-12-09T16:53:16Z

New page: '''Unreleased structure''' The entry 5mi0 is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5mi0 is ON HOLD until sometime in the future

Authors:

Description:
[[Category: Unreleased Structures]]