5mpi - Revision history

OCA at 17:43, 8 November 2023

2023-11-08T17:43:06Z

←Older revision		Revision as of 17:43, 8 November 2023
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	==Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily==		==Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily==
-	<StructureSection load='5mpi' size='340' side='right' caption='[[5mpi]], [[Resolution\|resolution]] 2.34Å' scene=''>	+	<StructureSection load='5mpi' size='340' side='right'caption='[[5mpi]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mpi]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MPI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MPI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mpi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MPI FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~GRHL1~~, ~~LBP32, MGR, TFCP2L2 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.345Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mpi OCA], [~~http~~://pdbe.org/5mpi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mpi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mpi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mpi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mpi OCA], [https://pdbe.org/5mpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mpi RCSB], [https://www.ebi.ac.uk/pdbsum/5mpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mpi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GRHL1_HUMAN GRHL1_HUMAN]] Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:12175488, PubMed:18288204). Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (By similarity). Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed (PubMed:12175488).[UniProtKB:Q921D9]<ref>PMID:12175488</ref> <ref>PMID:18288204</ref>	+	[https://www.uniprot.org/uniprot/GRHL1_HUMAN GRHL1_HUMAN] Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:12175488, PubMed:18288204). Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (By similarity). Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed (PubMed:12175488).[UniProtKB:Q921D9]<ref>PMID:12175488</ref> <ref>PMID:18288204</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: Heinemann, U]]	+	[[Category: Large Structures]]
-	[[Category: Ibraimi, I]]	+	[[Category: Heinemann U]]
-	[[Category: Ming, Q]]	+	[[Category: Ibraimi I]]
-	[[Category: Roske, Y]]	+	[[Category: Ming Q]]
-	[[Category: Schmidt-Ott~~, K M~~]]	+	[[Category: Roske Y]]
-	[[Category: Schuetz, A]]	+	[[Category: Schmidt-Ott KM]]
-	[[Category: Walentin, K]]	+	[[Category: Schuetz A]]
-	~~[[Category: Dna-binding domain]]~~	+	[[Category: Walentin K]]
-	~~[[Category: Grainyhead]]~~	+
-	~~[[Category: Grhl1]]~~	+
-	~~[[Category: R427q]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription factor~~]]	+

OCA at 06:48, 14 March 2018

2018-03-14T06:48:34Z

←Older revision		Revision as of 06:48, 14 March 2018
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	<StructureSection load='5mpi' size='340' side='right' caption='[[5mpi]], [[Resolution\|resolution]] 2.34Å' scene=''>		<StructureSection load='5mpi' size='340' side='right' caption='[[5mpi]], [[Resolution\|resolution]] 2.34Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mpi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MPI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MPI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mpi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MPI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MPI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mpi OCA], [http://pdbe.org/5mpi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mpi RCSB], [http://www.ebi.ac.uk/pdbsum/5mpi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mpi ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GRHL1, LBP32, MGR, TFCP2L2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mpi OCA], [http://pdbe.org/5mpi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mpi RCSB], [http://www.ebi.ac.uk/pdbsum/5mpi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mpi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]
	[[Category: Ibraimi, I]]		[[Category: Ibraimi, I]]

OCA at 06:46, 17 January 2018

2018-01-17T06:46:51Z

←Older revision		Revision as of 06:46, 17 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mpi is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily==
		+	<StructureSection load='5mpi' size='340' side='right' caption='[[5mpi]], [[Resolution\|resolution]] 2.34Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mpi]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MPI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MPI FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mpi OCA], [http://pdbe.org/5mpi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mpi RCSB], [http://www.ebi.ac.uk/pdbsum/5mpi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mpi ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GRHL1_HUMAN GRHL1_HUMAN]] Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' (PubMed:12175488, PubMed:18288204). Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development (By similarity). Isoform 1 may function as an activator and isoform 2 as a repressor in tissues where both forms are expressed (PubMed:12175488).[UniProtKB:Q921D9]<ref>PMID:12175488</ref> <ref>PMID:18288204</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Grainyhead (Grh)/CP2 transcription factors are highly conserved in multicellular organisms as key regulators of epithelial differentiation, organ development and skin barrier formation. In addition, they have been implicated as being tumor suppressors in a variety of human cancers. Despite their physiological importance, little is known about their structure and DNA binding mode. Here, we report the first structural study of mammalian Grh/CP2 factors. Crystal structures of the DNA-binding domains of grainyhead-like (Grhl) 1 and Grhl2 reveal a closely similar conformation with immunoglobulin-like core. Both share a common fold with the tumor suppressor p53, but differ in important structural features. The Grhl1 DNA-binding domain binds duplex DNA containing the consensus recognition element in a dimeric arrangement, supporting parsimonious target-sequence selection through two conserved arginine residues. We elucidate the molecular basis of a cancer-related mutation in Grhl1 involving one of these arginines, which completely abrogates DNA binding in biochemical assays and transcriptional activation of a reporter gene in a human cell line. Thus, our studies establish the structural basis of DNA target-site recognition by Grh transcription factors and reveal how tumor-associated mutations inactivate Grhl proteins. They may serve as points of departure for the structure-based development of Grh/CP2 inhibitors for therapeutic applications.

-	~~Authors: Ming~~, Q., Roske, Y., Schuetz, A., Walentin, K., Ibraimi, I., Schmidt-Ott~~, K.M.~~, Heinemann, U.	+	Structural basis of gene regulation by the Grainyhead/CP2 transcription factor family.,Ming Q, Roske Y, Schuetz A, Walentin K, Ibraimi I, Schmidt-Ott KM, Heinemann U Nucleic Acids Res. 2018 Jan 4. pii: 4788346. doi: 10.1093/nar/gkx1299. PMID:29309642<ref>PMID:29309642</ref>

-	~~Description: Structural Basis~~ of ~~Gene Regulation by~~ the ~~Grainyhead Transcription Factor Superfamily~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Walentin, K]]~~	+	<div class="pdbe-citations 5mpi" style="background-color:#fffaf0;"></div>
-	~~[[Category: Ming, Q]]~~	+	== References ==
-	~~[[Category: Ibraimi, I]]~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]
-	[[Category: ~~Schmidt-Ott~~, ~~K.M~~]]	+	[[Category: Ibraimi, I]]
		+	[[Category: Ming, Q]]
	[[Category: Roske, Y]]		[[Category: Roske, Y]]
		+	[[Category: Schmidt-Ott, K M]]
	[[Category: Schuetz, A]]		[[Category: Schuetz, A]]
		+	[[Category: Walentin, K]]
		+	[[Category: Dna-binding domain]]
		+	[[Category: Grainyhead]]
		+	[[Category: Grhl1]]
		+	[[Category: R427q]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription factor]]

OCA at 11:07, 27 September 2017

2017-09-27T11:07:41Z

←Older revision		Revision as of 11:07, 27 September 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mpi is ON HOLD	+	The entry 5mpi is ON HOLD until Paper Publication

	Authors: Ming, Q., Roske, Y., Schuetz, A., Walentin, K., Ibraimi, I., Schmidt-Ott, K.M., Heinemann, U.		Authors: Ming, Q., Roske, Y., Schuetz, A., Walentin, K., Ibraimi, I., Schmidt-Ott, K.M., Heinemann, U.

OCA: Protected "5mpi" [edit=sysop:move=sysop]

2017-01-02T13:49:47Z

Protected "5mpi" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:49, 2 January 2017

OCA: New page: '''Unreleased structure''' The entry 5mpi is ON HOLD Authors: Ming, Q., Roske, Y., Schuetz, A., Walentin, K., Ibraimi, I., Schmidt-Ott, K.M., Heinemann, U. Description: Structural Basi...

2017-01-02T13:49:46Z

New page: '''Unreleased structure''' The entry 5mpi is ON HOLD Authors: Ming, Q., Roske, Y., Schuetz, A., Walentin, K., Ibraimi, I., Schmidt-Ott, K.M., Heinemann, U. Description: Structural Basi...

New page

'''Unreleased structure'''

The entry 5mpi is ON HOLD

Authors: Ming, Q., Roske, Y., Schuetz, A., Walentin, K., Ibraimi, I., Schmidt-Ott, K.M., Heinemann, U.

Description: Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily
[[Category: Unreleased Structures]]
[[Category: Walentin, K]]
[[Category: Ming, Q]]
[[Category: Ibraimi, I]]
[[Category: Heinemann, U]]
[[Category: Schmidt-Ott, K.M]]
[[Category: Roske, Y]]
[[Category: Schuetz, A]]