5mrv - Revision history

OCA at 17:47, 8 November 2023

2023-11-08T17:47:43Z

←Older revision		Revision as of 17:47, 8 November 2023
Line 1:		Line 1:

	==Crystal structure of human carboxypeptidase O in complex with NvCI==		==Crystal structure of human carboxypeptidase O in complex with NvCI==
-	<StructureSection load='5mrv' size='340' side='right' caption='[[5mrv]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='5mrv' size='340' side='right'caption='[[5mrv]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mrv]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Four-tooth_nerite Four-tooth nerite~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MRV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MRV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mrv]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Nerita_versicolor Nerita versicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MRV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=NAG:N~~-~~ACETYL-D-GLUCOSAMINE'>NAG</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.854Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CPO ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mrv OCA], [~~http~~://pdbe.org/5mrv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mrv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mrv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mrv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mrv OCA], [https://pdbe.org/5mrv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mrv RCSB], [https://www.ebi.ac.uk/pdbsum/5mrv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mrv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CBPO_HUMAN CBPO_HUMAN]] Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.<ref>PMID:21921028</ref> [[http://www.uniprot.org/uniprot/MCPI_NERVS MCPI_NERVS]] Metallocarboxypeptidase inhibitor. Has an inhibitory effect on bovine CPA1 and CPB2, human CPA1, CPA2, CPA4, CPB1 and CPB2, and porcine CPB1. Does not inhibit D.melanogaster svr (carboxypeptidase D). Shows no activity against serine proteases subtilisin or bovine trypsin, cysteine protease papain, and aspartyl protease porcine pepsin.<ref>PMID:22294694</ref> [UniProtKB:P01075]	+	[https://www.uniprot.org/uniprot/CBPO_HUMAN CBPO_HUMAN] Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.<ref>PMID:21921028</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Carboxypeptidase\|Carboxypeptidase]]	+	*[[Carboxypeptidase 3D structures\|Carboxypeptidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Four-tooth nerite~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Human~~]]	+	[[Category: Large Structures]]
-	[[Category: Aviles~~, F X~~]]	+	[[Category: Nerita versicolor]]
-	[[Category: Fernandez-Alvarez, R]]	+	[[Category: Aviles FX]]
-	[[Category: Garcia-Guerrero~~, M C~~]]	+	[[Category: Fernandez-Alvarez R]]
-	[[Category: Garcia-Pardo, J]]	+	[[Category: Garcia-Guerrero MC]]
-	[[Category: Lorenzo, J]]	+	[[Category: Garcia-Pardo J]]
-	[[Category: Lyons, P]]	+	[[Category: Lorenzo J]]
-	[[Category: Reverter, D]]	+	[[Category: Lyons P]]
-	~~[[Category: Brush border enzyme]]~~	+	[[Category: Reverter D]]
-	~~[[Category: Carboxypeptidase inhibitor]]~~	+
-	~~[[Category: Digestive metallocarboxypeptidase]]~~	+
-	~~[[Category: Food digestion]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 05:47, 16 May 2018

2018-05-16T05:47:00Z

←Older revision		Revision as of 05:47, 16 May 2018
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	<StructureSection load='5mrv' size='340' side='right' caption='[[5mrv]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='5mrv' size='340' side='right' caption='[[5mrv]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mrv]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MRV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mrv]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Four-tooth_nerite Four-tooth nerite] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MRV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CPO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mrv OCA], [http://pdbe.org/5mrv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mrv RCSB], [http://www.ebi.ac.uk/pdbsum/5mrv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mrv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mrv OCA], [http://pdbe.org/5mrv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mrv RCSB], [http://www.ebi.ac.uk/pdbsum/5mrv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mrv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CBPO_HUMAN CBPO_HUMAN]] Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.<ref>PMID:21921028</ref> [[http://www.uniprot.org/uniprot/MCPI_NERVS MCPI_NERVS]] Metallocarboxypeptidase inhibitor. Has an inhibitory effect on bovine CPA1 and CPB2, human CPA1, CPA2, CPA4, CPB1 and CPB2, and porcine CPB1. Does not inhibit D.melanogaster svr (carboxypeptidase D). Shows no activity against serine proteases subtilisin or bovine trypsin, cysteine protease papain, and aspartyl protease porcine pepsin.<ref>PMID:22294694</ref> [UniProtKB:P01075]		[[http://www.uniprot.org/uniprot/CBPO_HUMAN CBPO_HUMAN]] Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.<ref>PMID:21921028</ref> [[http://www.uniprot.org/uniprot/MCPI_NERVS MCPI_NERVS]] Metallocarboxypeptidase inhibitor. Has an inhibitory effect on bovine CPA1 and CPB2, human CPA1, CPA2, CPA4, CPB1 and CPB2, and porcine CPB1. Does not inhibit D.melanogaster svr (carboxypeptidase D). Shows no activity against serine proteases subtilisin or bovine trypsin, cysteine protease papain, and aspartyl protease porcine pepsin.<ref>PMID:22294694</ref> [UniProtKB:P01075]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human metallocarboxypeptidase O (hCPO) is a recently discovered digestive enzyme localized to the apical membrane of intestinal epithelial cells. Unlike pancreatic metallocarboxypeptidases, hCPO is glycosylated and produced as an active enzyme with distinctive substrate specificity toward C-terminal (C-t) acidic residues. Here we present the crystal structure of hCPO at 1.85-A resolution, both alone and in complex with a carboxypeptidase inhibitor (NvCI) from the marine snail Nerita versicolor The structure provides detailed information regarding determinants of enzyme specificity, in particular Arg275, placed at the bottom of the substrate-binding pocket. This residue, located at "canonical" position 255, where it is Ile in human pancreatic carboxypeptidases A1 (hCPA1) and A2 (hCPA2) and Asp in B (hCPB), plays a dominant role in determining the preference of hCPO for acidic C-t residues. Site-directed mutagenesis to Asp and Ala changes the specificity to C-t basic and hydrophobic residues, respectively. The single-site mutants thus faithfully mimic the enzymatic properties of CPB and CPA, respectively. hCPO also shows a preference for Glu over Asp, probably as a consequence of a tighter fitting of the Glu side chain in its S1' substrate-binding pocket. This unique preference of hCPO, together with hCPA1, hCPA2, and hCPB, completes the array of C-t cleavages enabling the digestion of the dietary proteins within the intestine. Finally, in addition to activity toward small synthetic substrates and peptides, hCPO can also trim C-t extensions of proteins, such as epidermal growth factor, suggesting a role in the maturation and degradation of growth factors and bioactive peptides.
		+
		+	Crystal structure and mechanism of human carboxypeptidase O: Insights into its specific activity for acidic residues.,Garcia-Guerrero MC, Garcia-Pardo J, Berenguer E, Fernandez-Alvarez R, Barfi GB, Lyons PJ, Aviles FX, Huber R, Lorenzo J, Reverter D Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E3932-E3939. doi:, 10.1073/pnas.1803685115. Epub 2018 Apr 10. PMID:29636417<ref>PMID:29636417</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5mrv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Carboxypeptidase\|Carboxypeptidase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Four-tooth nerite]]
		+	[[Category: Human]]
	[[Category: Aviles, F X]]		[[Category: Aviles, F X]]
	[[Category: Fernandez-Alvarez, R]]		[[Category: Fernandez-Alvarez, R]]

OCA at 06:48, 17 January 2018

2018-01-17T06:48:50Z

←Older revision		Revision as of 06:48, 17 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mrv ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of human carboxypeptidase O in complex with NvCI==
-		+	<StructureSection load='5mrv' size='340' side='right' caption='[[5mrv]], [[Resolution\|resolution]] 1.85Å' scene=''>
-	~~Authors~~: ~~Garcia~~-~~Pardo, J~~.~~, Garcia~~-~~Guerrero~~, M.C., ~~Fernandez~~-~~Alvarez~~, R., ~~Lyons~~, P., ~~Aviles~~, F.X., ~~Lorenzo~~, J., ~~Reverter~~, D.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5mrv]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MRV FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of human carboxypeptidase O in complex with NvCI~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mrv OCA], [http://pdbe.org/5mrv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mrv RCSB], [http://www.ebi.ac.uk/pdbsum/5mrv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mrv ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CBPO_HUMAN CBPO_HUMAN]] Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.<ref>PMID:21921028</ref> [[http://www.uniprot.org/uniprot/MCPI_NERVS MCPI_NERVS]] Metallocarboxypeptidase inhibitor. Has an inhibitory effect on bovine CPA1 and CPB2, human CPA1, CPA2, CPA4, CPB1 and CPB2, and porcine CPB1. Does not inhibit D.melanogaster svr (carboxypeptidase D). Shows no activity against serine proteases subtilisin or bovine trypsin, cysteine protease papain, and aspartyl protease porcine pepsin.<ref>PMID:22294694</ref> [UniProtKB:P01075]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aviles, F X]]
		+	[[Category: Fernandez-Alvarez, R]]
		+	[[Category: Garcia-Guerrero, M C]]
		+	[[Category: Garcia-Pardo, J]]
	[[Category: Lorenzo, J]]		[[Category: Lorenzo, J]]
-	[[Category: ~~Aviles~~, ~~F.X~~]]	+	[[Category: Lyons, P]]
	[[Category: Reverter, D]]		[[Category: Reverter, D]]
-	[[Category: ~~Garcia-Guerrero, M.C~~]]	+	[[Category: Brush border enzyme]]
-	[[Category: ~~Garcia-Pardo, J~~]]	+	[[Category: Carboxypeptidase inhibitor]]
-	[[Category: ~~Lyons, P~~]]	+	[[Category: Digestive metallocarboxypeptidase]]
-	[[Category: ~~Fernandez-Alvarez, R~~]]	+	[[Category: Food digestion]]
		+	[[Category: Hydrolase]]

OCA at 11:35, 9 March 2017

2017-03-09T11:35:32Z

←Older revision		Revision as of 11:35, 9 March 2017
Line 7:		Line 7:
	Description: Crystal structure of human carboxypeptidase O in complex with NvCI		Description: Crystal structure of human carboxypeptidase O in complex with NvCI
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Reverter, D]]
-	[[Category: Garcia-Pardo, J]]
-	[[Category: Lyons, P]]
	[[Category: Lorenzo, J]]		[[Category: Lorenzo, J]]
	[[Category: Aviles, F.X]]		[[Category: Aviles, F.X]]
		+	[[Category: Reverter, D]]
	[[Category: Garcia-Guerrero, M.C]]		[[Category: Garcia-Guerrero, M.C]]
		+	[[Category: Garcia-Pardo, J]]
		+	[[Category: Lyons, P]]
	[[Category: Fernandez-Alvarez, R]]		[[Category: Fernandez-Alvarez, R]]

OCA at 20:27, 25 January 2017

2017-01-25T20:27:07Z

←Older revision		Revision as of 20:27, 25 January 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mrv is ON HOLD	+	The entry 5mrv is ON HOLD until Paper Publication

	Authors: Garcia-Pardo, J., Garcia-Guerrero, M.C., Fernandez-Alvarez, R., Lyons, P., Aviles, F.X., Lorenzo, J., Reverter, D.		Authors: Garcia-Pardo, J., Garcia-Guerrero, M.C., Fernandez-Alvarez, R., Lyons, P., Aviles, F.X., Lorenzo, J., Reverter, D.

OCA at 13:08, 18 January 2017

2017-01-18T13:08:03Z

←Older revision		Revision as of 13:08, 18 January 2017
Line 3:		Line 3:
	The entry 5mrv is ON HOLD		The entry 5mrv is ON HOLD

-	Authors: Garcia-Pardo, J., Garcia-Guerrero, M.C., Fernandez-Alvarez, R., Lyons, P., Aviles, F.X., Lorenzo J., Reverter, D.	+	Authors: Garcia-Pardo, J., Garcia-Guerrero, M.C., Fernandez-Alvarez, R., Lyons, P., Aviles, F.X., Lorenzo, J., Reverter, D.

	Description: Crystal structure of human carboxypeptidase O in complex with NvCI		Description: Crystal structure of human carboxypeptidase O in complex with NvCI
Line 10:		Line 10:
	[[Category: Garcia-Pardo, J]]		[[Category: Garcia-Pardo, J]]
	[[Category: Lyons, P]]		[[Category: Lyons, P]]
-	[[Category: Lorenzo J]]	+	[[Category: Lorenzo, J]]
	[[Category: Aviles, F.X]]		[[Category: Aviles, F.X]]
	[[Category: Garcia-Guerrero, M.C]]		[[Category: Garcia-Guerrero, M.C]]
	[[Category: Fernandez-Alvarez, R]]		[[Category: Fernandez-Alvarez, R]]

OCA: Protected "5mrv" [edit=sysop:move=sysop]

2017-01-04T14:19:53Z

Protected "5mrv" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:19, 4 January 2017

OCA: New page: '''Unreleased structure''' The entry 5mrv is ON HOLD Authors: Garcia-Pardo, J., Garcia-Guerrero, M.C., Fernandez-Alvarez, R., Lyons, P., Aviles, F.X., Lorenzo J., Reverter, D. Descript...

2017-01-04T14:19:51Z

New page: '''Unreleased structure''' The entry 5mrv is ON HOLD Authors: Garcia-Pardo, J., Garcia-Guerrero, M.C., Fernandez-Alvarez, R., Lyons, P., Aviles, F.X., Lorenzo J., Reverter, D. Descript...

New page

'''Unreleased structure'''

The entry 5mrv is ON HOLD

Authors: Garcia-Pardo, J., Garcia-Guerrero, M.C., Fernandez-Alvarez, R., Lyons, P., Aviles, F.X., Lorenzo J., Reverter, D.

Description: Crystal structure of human carboxypeptidase O in complex with NvCI
[[Category: Unreleased Structures]]
[[Category: Reverter, D]]
[[Category: Garcia-Pardo, J]]
[[Category: Lyons, P]]
[[Category: Lorenzo J]]
[[Category: Aviles, F.X]]
[[Category: Garcia-Guerrero, M.C]]
[[Category: Fernandez-Alvarez, R]]