5mzo - Revision history

OCA at 17:55, 8 November 2023

2023-11-08T17:55:03Z

←Older revision		Revision as of 17:55, 8 November 2023
Line 1:		Line 1:

	==UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum (open conformation)==		==UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum (open conformation)==
-	<StructureSection load='5mzo' size='340' side='right' caption='[[5mzo]], [[Resolution\|resolution]] 3.48Å' scene=''>	+	<StructureSection load='5mzo' size='340' side='right'caption='[[5mzo]], [[Resolution\|resolution]] 3.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mzo]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chatd Chatd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5MZO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mzo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MZO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.48Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTHT_0048990 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5mzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzo OCA], [~~http~~://pdbe.org/5mzo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5mzo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5mzo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzo ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzo OCA], [https://pdbe.org/5mzo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mzo RCSB], [https://www.ebi.ac.uk/pdbsum/5mzo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0SB58_CHATD G0SB58_CHATD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chatd~~]]	+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
-	[[Category: Alonzi~~, D S~~]]	+	[[Category: Large Structures]]
-	[[Category: Caputo~~, A T~~]]	+	[[Category: Alonzi DS]]
-	[[Category: Hill, J]]	+	[[Category: Caputo AT]]
-	[[Category: Roversi, P]]	+	[[Category: Hill J]]
-	[[Category: Zitzmann, N]]	+	[[Category: Roversi P]]
-	~~[[Category: Endoplasmic reticulum]]~~	+	[[Category: Zitzmann N]]
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Misfolding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 01:22, 16 November 2017

2017-11-16T01:22:37Z

←Older revision		Revision as of 01:22, 16 November 2017
Line 3:		Line 3:
	<StructureSection load='5mzo' size='340' side='right' caption='[[5mzo]], [[Resolution\|resolution]] 3.48Å' scene=''>		<StructureSection load='5mzo' size='340' side='right' caption='[[5mzo]], [[Resolution\|resolution]] 3.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5mzo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MZO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5mzo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MZO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTHT_0048990 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzo OCA], [http://pdbe.org/5mzo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mzo RCSB], [http://www.ebi.ac.uk/pdbsum/5mzo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzo OCA], [http://pdbe.org/5mzo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mzo RCSB], [http://www.ebi.ac.uk/pdbsum/5mzo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzo ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chatd]]
	[[Category: Alonzi, D S]]		[[Category: Alonzi, D S]]
	[[Category: Caputo, A T]]		[[Category: Caputo, A T]]

OCA at 03:59, 4 August 2017

2017-08-04T03:59:51Z

←Older revision		Revision as of 03:59, 4 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5mzo is ~~ON HOLD until Paper~~ Publication	+	==UDP-Glucose Glycoprotein Glucosyltransferase from Chaetomium thermophilum (open conformation)==
		+	<StructureSection load='5mzo' size='340' side='right' caption='[[5mzo]], [[Resolution\|resolution]] 3.48Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5mzo]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MZO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mzo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzo OCA], [http://pdbe.org/5mzo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mzo RCSB], [http://www.ebi.ac.uk/pdbsum/5mzo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzo ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glycoproteins traversing the eukaryotic secretory pathway begin life in the endoplasmic reticulum (ER), where their folding is surveyed by the 170-kDa UDP-glucose:glycoprotein glucosyltransferase (UGGT). The enzyme acts as the single glycoprotein folding quality control checkpoint: it selectively reglucosylates misfolded glycoproteins, promotes their association with ER lectins and associated chaperones, and prevents premature secretion from the ER. UGGT has long resisted structural determination and sequence-based domain boundary prediction. Questions remain on how this single enzyme can flag misfolded glycoproteins of different sizes and shapes for ER retention and how it can span variable distances between the site of misfold and a glucose-accepting N-linked glycan on the same glycoprotein. Here, crystal structures of a full-length eukaryotic UGGT reveal four thioredoxin-like (TRXL) domains arranged in a long arc that terminates in two beta-sandwiches tightly clasping the glucosyltransferase domain. The fold of the molecule is topologically complex, with the first beta-sandwich and the fourth TRXL domain being encoded by nonconsecutive stretches of sequence. In addition to the crystal structures, a 15-A cryo-EM reconstruction reveals interdomain flexibility of the TRXL domains. Double cysteine point mutants that engineer extra interdomain disulfide bridges rigidify the UGGT structure and exhibit impaired activity. The intrinsic flexibility of the TRXL domains of UGGT may therefore endow the enzyme with the promiscuity needed to recognize and reglucosylate its many different substrates and/or enable reglucosylation of N-linked glycans situated at variable distances from the site of misfold.

-	~~Authors~~:	+	Interdomain conformational flexibility underpins the activity of UGGT, the eukaryotic glycoprotein secretion checkpoint.,Roversi P, Marti L, Caputo AT, Alonzi DS, Hill JC, Dent KC, Kumar A, Levasseur MD, Lia A, Waksman T, Basu S, Soto Albrecht Y, Qian K, McIvor JP, Lipp CB, Siliqi D, Vasiljevic S, Mohammed S, Lukacik P, Walsh MA, Santino A, Zitzmann N Proc Natl Acad Sci U S A. 2017 Jul 24. pii: 201703682. doi:, 10.1073/pnas.1703682114. PMID:28739903<ref>PMID:28739903</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5mzo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Alonzi, D S]]
		+	[[Category: Caputo, A T]]
		+	[[Category: Hill, J]]
		+	[[Category: Roversi, P]]
		+	[[Category: Zitzmann, N]]
		+	[[Category: Endoplasmic reticulum]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Misfolding]]
		+	[[Category: Transferase]]

OCA at 12:38, 22 March 2017

2017-03-22T12:38:26Z

←Older revision		Revision as of 12:38, 22 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5mzo is ON HOLD	+	The entry 5mzo is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5mzo" [edit=sysop:move=sysop]

2017-03-09T11:50:24Z

Protected "5mzo" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:50, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5mzo is ON HOLD Authors: Description: Category: Unreleased Structures

2017-03-09T11:50:23Z

New page: '''Unreleased structure''' The entry 5mzo is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5mzo is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]