5n6l - Revision history

OCA at 12:29, 15 November 2023

2023-11-15T12:29:37Z

←Older revision		Revision as of 12:29, 15 November 2023
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	<StructureSection load='5n6l' size='340' side='right'caption='[[5n6l]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='5n6l' size='340' side='right'caption='[[5n6l]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n6l]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N6L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n6l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N6L FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=GOL:GLYCEROL'>GOL</scene~~>, ~~<scene name='pdbligand=OLC:(2R)-~~2~~,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-~~9~~-ENOATE'>OLC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~lnt, cutE, b0657, JW0654 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83333 ECOLI]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n6l OCA], [~~http~~://pdbe.org/5n6l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n6l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n6l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n6l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n6l OCA], [https://pdbe.org/5n6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n6l RCSB], [https://www.ebi.ac.uk/pdbsum/5n6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n6l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LNT_ECOLI LNT_ECOLI]] Transfers the fatty acyl group on membrane lipoproteins.	+	[https://www.uniprot.org/uniprot/LNT_ECOLI LNT_ECOLI] Transfers the fatty acyl group on membrane lipoproteins.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bailey, J]]	+	[[Category: Bailey J]]
-	[[Category: Boland, C]]	+	[[Category: Boland C]]
-	[[Category: Caffrey, M]]	+	[[Category: Caffrey M]]
-	[[Category: Howe, N]]	+	[[Category: Howe N]]
-	[[Category: Huang, C Y]]	+	[[Category: Huang C-Y]]
-	[[Category: Olieric, V]]	+	[[Category: Olieric V]]
-	[[Category: Vogeley, L]]	+	[[Category: Vogeley L]]
-	[[Category: Wang, M]]	+	[[Category: Wang M]]
-	[[Category: Weichert, D]]	+	[[Category: Weichert D]]
-	[[Category: Wiktor, M]]	+	[[Category: Wiktor M]]
-	~~[[Category: Lipidic cubic phase]]~~	+
-	~~[[Category: Lipoprotein]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: N-acyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:42, 16 October 2019

2019-10-16T06:42:33Z

←Older revision		Revision as of 06:42, 16 October 2019
Line 1:		Line 1:

	==Structure of the membrane integral lipoprotein N-acyltransferase Lnt C387A mutant from E. coli==		==Structure of the membrane integral lipoprotein N-acyltransferase Lnt C387A mutant from E. coli==
-	<StructureSection load='5n6l' size='340' side='right' caption='[[5n6l]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='5n6l' size='340' side='right'caption='[[5n6l]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5n6l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N6L FirstGlance]. <br>		<table><tr><td colspan='2'>[[5n6l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N6L FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Bailey, J]]		[[Category: Bailey, J]]
	[[Category: Boland, C]]		[[Category: Boland, C]]

OCA at 06:58, 28 March 2018

2018-03-28T06:58:39Z

←Older revision		Revision as of 06:58, 28 March 2018
Line 3:		Line 3:
	<StructureSection load='5n6l' size='340' side='right' caption='[[5n6l]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='5n6l' size='340' side='right' caption='[[5n6l]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n6l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N6L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n6l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N6L FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lnt, cutE, b0657, JW0654 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n6l OCA], [http://pdbe.org/5n6l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n6l RCSB], [http://www.ebi.ac.uk/pdbsum/5n6l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n6l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n6l OCA], [http://pdbe.org/5n6l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n6l RCSB], [http://www.ebi.ac.uk/pdbsum/5n6l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n6l ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Bailey, J]]		[[Category: Bailey, J]]
	[[Category: Boland, C]]		[[Category: Boland, C]]
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	[[Category: Huang, C Y]]		[[Category: Huang, C Y]]
	[[Category: Olieric, V]]		[[Category: Olieric, V]]
-	[[Category: ~~Volgeley~~, L]]	+	[[Category: Vogeley, L]]
	[[Category: Wang, M]]		[[Category: Wang, M]]
	[[Category: Weichert, D]]		[[Category: Weichert, D]]

OCA at 10:09, 10 September 2017

2017-09-10T10:09:10Z

←Older revision		Revision as of 10:09, 10 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5n6l is ~~ON HOLD~~	+	==Structure of the membrane integral lipoprotein N-acyltransferase Lnt C387A mutant from E. coli==
		+	<StructureSection load='5n6l' size='340' side='right' caption='[[5n6l]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5n6l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N6L FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n6l OCA], [http://pdbe.org/5n6l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n6l RCSB], [http://www.ebi.ac.uk/pdbsum/5n6l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n6l ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LNT_ECOLI LNT_ECOLI]] Transfers the fatty acyl group on membrane lipoproteins.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lipoproteins serve essential roles in the bacterial cell envelope. The posttranslational modification pathway leading to lipoprotein synthesis involves three enzymes. All are potential targets for the development of new antibiotics. Here we report the crystal structure of the last enzyme in the pathway, apolipoprotein N-acyltransferase, Lnt, responsible for adding a third acyl chain to the lipoprotein's invariant diacylated N-terminal cysteine. Structures of Lnt from Pseudomonas aeruginosa and Escherichia coli have been solved; they are remarkably similar. Both consist of a membrane domain on which sits a globular periplasmic domain. The active site resides above the membrane interface where the domains meet facing into the periplasm. The structures are consistent with the proposed ping-pong reaction mechanism and suggest plausible routes by which substrates and products enter and leave the active site. While Lnt may present challenges for antibiotic development, the structures described should facilitate design of therapeutics with reduced off-target effects.

-	~~Authors~~:	+	Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis.,Wiktor M, Weichert D, Howe N, Huang CY, Olieric V, Boland C, Bailey J, Vogeley L, Stansfeld PJ, Buddelmeijer N, Wang M, Caffrey M Nat Commun. 2017 Jul 4;8:15952. doi: 10.1038/ncomms15952. PMID:28675161<ref>PMID:28675161</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5n6l" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bailey, J]]
		+	[[Category: Boland, C]]
		+	[[Category: Caffrey, M]]
		+	[[Category: Howe, N]]
		+	[[Category: Huang, C Y]]
		+	[[Category: Olieric, V]]
		+	[[Category: Volgeley, L]]
		+	[[Category: Wang, M]]
		+	[[Category: Weichert, D]]
		+	[[Category: Wiktor, M]]
		+	[[Category: Lipidic cubic phase]]
		+	[[Category: Lipoprotein]]
		+	[[Category: Membrane protein]]
		+	[[Category: N-acyltransferase]]
		+	[[Category: Transferase]]

OCA: Protected "5n6l" [edit=sysop:move=sysop]

2017-03-09T12:10:52Z

Protected "5n6l" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:10, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n6l is ON HOLD Authors: Description: Category: Unreleased Structures

2017-03-09T12:10:51Z

New page: '''Unreleased structure''' The entry 5n6l is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5n6l is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]