5n8k - Revision history

OCA: Redirecting to 5nxb

2017-05-24T13:33:38Z

Redirecting to 5nxb

←Older revision		Revision as of 13:33, 24 May 2017
Line 1:		Line 1:
-		+	#REDIRECT [[5nxb]] This PDB entry is obsolete and replaced by 5nxb
-	~~==Mouse galactocerebrosidase in complex with saposin A==~~	+
-	~~<StructureSection load='5n8k' size='340' side='right' caption='~~[[~~5n8k]], [[Resolution\|resolution]] 3.60Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[5n8k~~]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N8K FirstGlance]. <br>	+
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactosylceramidase Galactosylceramidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.46 3.2.1.46] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8k OCA], [http://pdbe.org/5n8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n8k RCSB], [http://www.ebi.ac.uk/pdbsum/5n8k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8k ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~== Disease ==~~	+
-	[[http://www.uniprot.org/uniprot/GALC_MOUSE GALC_MOUSE]] Defects in Galc are the cause of the 'twitcher' phenotype; an autosomal recessive leukodystrophy similar to the human disease (Krabbe disease). This deficiency results in the insufficient catabolism of several galactolipids that are important in the production of normal myelin.	+
-	~~== Function ==~~	+
-	~~[[http://www.uniprot.org/uniprot/GALC_MOUSE GALC_MOUSE]] Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide,~~ and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.<ref>PMID:8769874</ref> [[http://www.uniprot.org/uniprot/SAP_MOUSE SAP_MOUSE]] Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.<ref>PMID:23690594</ref> Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.[UniProtKB:P07602] Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.[UniProtKB:P07602] Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).[UniProtKB:P07602] Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.[UniProtKB:P07602]	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Galactosylceramidase]]~~	+
-	~~[[Category: Deane, J E]]~~	+
-	~~[[Category: Graham, S C]]~~	+
-	~~[[Category: Hill, C H]]~~	+
-	~~[[Category: Galactocerebroside]]~~	+
-	~~[[Category: Galactosylceramide]]~~	+
-	~~[[Category: Galc]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Krabbe disease]]~~	+
-	~~[[Category: Sapa]]~~	+

OCA at 21:21, 15 March 2017

2017-03-15T21:21:38Z

←Older revision		Revision as of 21:21, 15 March 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5n8k is ~~ON HOLD~~	+	==Mouse galactocerebrosidase in complex with saposin A==
-		+	<StructureSection load='5n8k' size='340' side='right' caption='[[5n8k]], [[Resolution\|resolution]] 3.60Å' scene=''>
-	~~Authors~~: ~~Graham~~, S.C., ~~Hill~~, C.H., ~~Deane~~, J.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5n8k]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N8K FirstGlance]. <br>
-	~~Description~~: ~~Mouse galactocerebrosidase~~ in ~~complex~~ with saposin A	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Galactosylceramidase Galactosylceramidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.46 3.2.1.46] </span></td></tr>
-	[[Category: Deane, J.E]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n8k OCA], [http://pdbe.org/5n8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n8k RCSB], [http://www.ebi.ac.uk/pdbsum/5n8k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n8k ProSAT]</span></td></tr>
-	[[Category: ~~Hill~~, C.H]]	+	</table>
-	[[Category: ~~Graham~~, S.C]]	+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/GALC_MOUSE GALC_MOUSE]] Defects in Galc are the cause of the 'twitcher' phenotype; an autosomal recessive leukodystrophy similar to the human disease (Krabbe disease). This deficiency results in the insufficient catabolism of several galactolipids that are important in the production of normal myelin.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GALC_MOUSE GALC_MOUSE]] Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.<ref>PMID:8769874</ref> [[http://www.uniprot.org/uniprot/SAP_MOUSE SAP_MOUSE]] Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.<ref>PMID:23690594</ref> Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.[UniProtKB:P07602] Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.[UniProtKB:P07602] Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).[UniProtKB:P07602] Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.[UniProtKB:P07602]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Galactosylceramidase]]
		+	[[Category: Deane, J E]]
		+	[[Category: Graham, S C]]
		+	[[Category: Hill, C H]]
		+	[[Category: Galactocerebroside]]
		+	[[Category: Galactosylceramide]]
		+	[[Category: Galc]]
		+	[[Category: Hydrolase]]
		+	[[Category: Krabbe disease]]
		+	[[Category: Sapa]]

OCA: Protected "5n8k" [edit=sysop:move=sysop]

2017-03-09T12:16:22Z

Protected "5n8k" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:16, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n8k is ON HOLD Authors: Graham, S.C., Hill, C.H., Deane, J.E. Description: Mouse galactocerebrosidase in complex with saposin A [[Category: Unrele...

2017-03-09T12:16:20Z

New page: '''Unreleased structure''' The entry 5n8k is ON HOLD Authors: Graham, S.C., Hill, C.H., Deane, J.E. Description: Mouse galactocerebrosidase in complex with saposin A [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 5n8k is ON HOLD

Authors: Graham, S.C., Hill, C.H., Deane, J.E.

Description: Mouse galactocerebrosidase in complex with saposin A
[[Category: Unreleased Structures]]
[[Category: Deane, J.E]]
[[Category: Hill, C.H]]
[[Category: Graham, S.C]]