5n9w - Revision history

OCA at 12:40, 15 November 2023

2023-11-15T12:40:08Z

←Older revision		Revision as of 12:40, 15 November 2023
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	<StructureSection load='5n9w' size='340' side='right'caption='[[5n9w]], [[Resolution\|resolution]] 2.46Å' scene=''>		<StructureSection load='5n9w' size='340' side='right'caption='[[5n9w]], [[Resolution\|resolution]] 2.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n9w]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5apl 5apl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5N9W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n9w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5apl 5apl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N9W FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.456Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5n9x\|5n9x]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5n9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9w OCA], [~~http~~://pdbe.org/5n9w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5n9w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5n9w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9w OCA], [https://pdbe.org/5n9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n9w RCSB], [https://www.ebi.ac.uk/pdbsum/5n9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/H6SG27_STRSQ H6SG27_STRSQ]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fullone~~, M R~~]]	+	[[Category: Streptomyces sp]]
-	[[Category: Grgurina, I]]	+	[[Category: Fullone MR]]
-	[[Category: Montemiglio~~, L C~~]]	+	[[Category: Grgurina I]]
-	[[Category: Parisi, G]]	+	[[Category: Montemiglio LC]]
-	[[Category: Savino, C]]	+	[[Category: Parisi G]]
-	[[Category: Scaglione, A]]	+	[[Category: Savino C]]
-	[[Category: Vallone, B]]	+	[[Category: Scaglione A]]
-	~~[[Category: Adenylation domain]]~~	+	[[Category: Vallone B]]
-	~~[[Category: Non-ribosomal code]]~~	+
-	~~[[Category: Substrate specificity]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:00, 26 August 2020

2020-08-26T12:00:09Z

←Older revision		Revision as of 12:00, 26 August 2020
Line 1:		Line 1:

	==Structure of adenylation domain THR1 involved in the biosynthesis of 4-chlorothreonine in Streptomyces SP.OH-5093, apo structure==		==Structure of adenylation domain THR1 involved in the biosynthesis of 4-chlorothreonine in Streptomyces SP.OH-5093, apo structure==
-	<StructureSection load='5n9w' size='340' side='right' caption='[[5n9w]], [[Resolution\|resolution]] 2.46Å' scene=''>	+	<StructureSection load='5n9w' size='340' side='right'caption='[[5n9w]], [[Resolution\|resolution]] 2.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5n9w]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5apl 5apl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9W OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5N9W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5n9w]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5apl 5apl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N9W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n9x\|5n9x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n9x\|5n9x]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5n9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9w OCA], [http://pdbe.org/5n9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n9w RCSB], [http://www.ebi.ac.uk/pdbsum/5n9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9w OCA], [http://pdbe.org/5n9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n9w RCSB], [http://www.ebi.ac.uk/pdbsum/5n9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9w ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Fullone, M R]]		[[Category: Fullone, M R]]
	[[Category: Grgurina, I]]		[[Category: Grgurina, I]]

OCA at 09:47, 27 September 2017

2017-09-27T09:47:24Z

←Older revision		Revision as of 09:47, 27 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5n9w is ~~ON HOLD until Paper~~ Publication	+	==Structure of adenylation domain THR1 involved in the biosynthesis of 4-chlorothreonine in Streptomyces SP.OH-5093, apo structure==
		+	<StructureSection load='5n9w' size='340' side='right' caption='[[5n9w]], [[Resolution\|resolution]] 2.46Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5n9w]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5apl 5apl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N9W FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n9x\|5n9x]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n9w OCA], [http://pdbe.org/5n9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n9w RCSB], [http://www.ebi.ac.uk/pdbsum/5n9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n9w ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We determined the crystal structure of Thr1, the self-standing adenylation domain involved in the nonribosomal-like biosynthesis of free 4-chlorothreonine in Streptomyces sp. OH-5093. Thr1 shows two monomers in the crystallographic asymmetric unit with different relative orientations of the C- and N-terminal subdomains both in the presence of substrates and in the unliganded form. Cocrystallization with substrates, adenosine 5'-triphosphate and l-threonine, yielded one monomer containing the two substrates and the other in complex with l-threonine adenylate, locked in a postadenylation state. Steady-state kinetics showed that Thr1 activates l-Thr and its stereoisomers, as well as d-Ala, l- and d-Ser, albeit with lower efficiency. Modeling of these substrates in the active site highlighted the molecular bases of substrate discrimination. This work provides the first crystal structure of a threonine-activating adenylation enzyme, a contribution to the studies on conformational rearrangement in adenylation domains and on substrate recognition in nonribosomal biosynthesis. DATABASE: Structural data are available in the Protein Data Bank under the accession number 5N9W and 5N9X.

-	~~Authors: Savino, C~~.~~, Vallone, B~~., Scaglione, A., Parisi, G., ~~Montemiglio, L.~~C., ~~Fullone~~, ~~M.R.~~, Grgurina, I.	+	Structure of the adenylation domain Thr1 involved in the biosynthesis of 4-chlorothreonine in Streptomyces sp. OH-5093-protein flexibility and molecular bases of substrate specificity.,Scaglione A, Fullone MR, Montemiglio LC, Parisi G, Zamparelli C, Vallone B, Savino C, Grgurina I FEBS J. 2017 Sep;284(18):2981-2999. doi: 10.1111/febs.14163. Epub 2017 Aug 7. PMID:28704585<ref>PMID:28704585</ref>

-	~~Description: Structure~~ of ~~adenylation domain THR1 involved in~~ the ~~biosynthesis~~ of ~~4-chlorothreonine in Streptomyces SP~~.OH-~~5093~~, ~~apo structure~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Montemiglio, L.C]]	+	<div class="pdbe-citations 5n9w" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fullone, M R]]
		+	[[Category: Grgurina, I]]
		+	[[Category: Montemiglio, L C]]
		+	[[Category: Parisi, G]]
	[[Category: Savino, C]]		[[Category: Savino, C]]
	[[Category: Scaglione, A]]		[[Category: Scaglione, A]]
	[[Category: Vallone, B]]		[[Category: Vallone, B]]
-	[[Category: ~~Parisi, G~~]]	+	[[Category: Adenylation domain]]
-	[[Category: ~~Grgurina, I~~]]	+	[[Category: Non-ribosomal code]]
-	[[Category: ~~Fullone, M.R~~]]	+	[[Category: Substrate specificity]]
		+	[[Category: Transferase]]

OCA at 09:49, 5 April 2017

2017-04-05T09:49:32Z

←Older revision		Revision as of 09:49, 5 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5n9w is ON HOLD	+	The entry 5n9w is ON HOLD until Paper Publication

	Authors: Savino, C., Vallone, B., Scaglione, A., Parisi, G., Montemiglio, L.C., Fullone, M.R., Grgurina, I.		Authors: Savino, C., Vallone, B., Scaglione, A., Parisi, G., Montemiglio, L.C., Fullone, M.R., Grgurina, I.

OCA: Protected "5n9w" [edit=sysop:move=sysop]

2017-03-09T12:20:09Z

Protected "5n9w" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:20, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5n9w is ON HOLD Authors: Savino, C., Vallone, B., Scaglione, A., Parisi, G., Montemiglio, L.C., Fullone, M.R., Grgurina, I. Description: Structure ...

2017-03-09T12:20:08Z

New page: '''Unreleased structure''' The entry 5n9w is ON HOLD Authors: Savino, C., Vallone, B., Scaglione, A., Parisi, G., Montemiglio, L.C., Fullone, M.R., Grgurina, I. Description: Structure ...

New page

'''Unreleased structure'''

The entry 5n9w is ON HOLD

Authors: Savino, C., Vallone, B., Scaglione, A., Parisi, G., Montemiglio, L.C., Fullone, M.R., Grgurina, I.

Description: Structure of adenylation domain THR1 involved in the biosynthesis of 4-chlorothreonine in Streptomyces SP.OH-5093, apo structure
[[Category: Unreleased Structures]]
[[Category: Montemiglio, L.C]]
[[Category: Savino, C]]
[[Category: Scaglione, A]]
[[Category: Vallone, B]]
[[Category: Parisi, G]]
[[Category: Grgurina, I]]
[[Category: Fullone, M.R]]