5nc1 - Revision history

OCA at 12:45, 15 November 2023

2023-11-15T12:45:52Z

←Older revision		Revision as of 12:45, 15 November 2023
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	<StructureSection load='5nc1' size='340' side='right'caption='[[5nc1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5nc1' size='340' side='right'caption='[[5nc1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nc1]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Murine_adenovirus_2 Murine adenovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NC1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nc1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_adenovirus_2 Murine adenovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NC1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n83\|5n83]], [[5n8d\|5n8d]], [[5nbh\|5nbh]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nc1 OCA], [~~http~~://pdbe.org/5nc1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nc1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nc1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nc1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nc1 OCA], [https://pdbe.org/5nc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nc1 RCSB], [https://www.ebi.ac.uk/pdbsum/5nc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nc1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/E7CH51_9ADEN E7CH51_9ADEN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Murine adenovirus 2]]		[[Category: Murine adenovirus 2]]
-	~~[[Category: Raaij, M J.van]]~~	+	[[Category: Singh AK]]
-	[[Category: Singh~~, A K~~]]	+	[[Category: Van Raaij MJ]]
-	[[Category: ~~3-bladed propeller]]~~	+
-	~~[[Category: Abcj-ghid topology]]~~	+
-	~~[[Category: N-acetyl-glucosamine binding]]~~	+
-	~~[[Category: Triple beta-spiral]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 12:01, 26 August 2020

2020-08-26T12:01:59Z

←Older revision		Revision as of 12:01, 26 August 2020
Line 1:		Line 1:

	==Structure of the distal domain of mouse adenovirus 2 fibre bound to N-acetyl-glucosamine==		==Structure of the distal domain of mouse adenovirus 2 fibre bound to N-acetyl-glucosamine==
-	<StructureSection load='5nc1' size='340' side='right' caption='[[5nc1]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5nc1' size='340' side='right'caption='[[5nc1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nc1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_adenovirus_2 Murine adenovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NC1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nc1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_adenovirus_2 Murine adenovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NC1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NC1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n83\|5n83]], [[5n8d\|5n8d]], [[5nbh\|5nbh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n83\|5n83]], [[5n8d\|5n8d]], [[5nbh\|5nbh]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nc1 OCA], [http://pdbe.org/5nc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/5nc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nc1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nc1 OCA], [http://pdbe.org/5nc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/5nc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nc1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Murine adenovirus 2]]		[[Category: Murine adenovirus 2]]
	[[Category: Raaij, M J.van]]		[[Category: Raaij, M J.van]]

OCA at 08:23, 10 October 2018

2018-10-10T08:23:52Z

←Older revision		Revision as of 08:23, 10 October 2018
Line 3:		Line 3:
	<StructureSection load='5nc1' size='340' side='right' caption='[[5nc1]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5nc1' size='340' side='right' caption='[[5nc1]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nc1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nc1]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_adenovirus_2 Murine adenovirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NC1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n83\|5n83]], [[5n8d\|5n8d]], [[5nbh\|5nbh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n83\|5n83]], [[5n8d\|5n8d]], [[5nbh\|5nbh]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nc1 OCA], [http://pdbe.org/5nc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/5nc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nc1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nc1 OCA], [http://pdbe.org/5nc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/5nc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nc1 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Murine adenovirus 2 (MAdV-2) infects cells of the mouse gastrointestinal tract. Like human adenoviruses, it is a member of the genus Mastadenovirus, family Adenoviridae. The MAdV-2 genome has a single fibre gene that expresses a 787 residue-long protein. Through analogy to other adenovirus fibre proteins, it is expected that the carboxy-terminal virus-distal head domain of the fibre is responsible for binding to the host cell, although the natural receptor is unknown. The putative head domain has little sequence identity to adenovirus fibres of known structure. In this report, we present high-resolution crystal structures of the carboxy-terminal part of the MAdV-2 fibre. The structures reveal a domain with the typical adenovirus fibre head topology and a domain containing two triple beta-spiral repeats of the shaft domain. Through glycan microarray profiling, saturation transfer difference nuclear magnetic resonance spectroscopy, isothermal titration calorimetry and site-directed mutagenesis, we show that the fibre specifically binds to the monosaccharide N-acetylglucosamine (GlcNAc). The crystal structure of the complex reveals that GlcNAc binds between the AB and CD loops at the top of each of the three monomers of the MAdV-2 fibre head. However, infection competition assays show that soluble GlcNAc monosaccharide and natural GlcNAc-containing polymers do not inhibit infection by MAdV-2. Furthermore, site-directed mutation of the GlcNAc-binding residues does not prevent the inhibition of infection by soluble fibre protein. On the other hand, we show that the MAdV-2 fibre protein binds GlcNAc-containing mucin glycans, which suggests that the MAdV-2 fibre protein may play a role in viral mucin penetration in the mouse gut.
		+
		+	Structure and N-acetylglucosamine binding of the distal domain of mouse adenovirus 2 fibre.,Singh AK, Nguyen TH, Vidovszky MZ, Harrach B, Benko M, Kirwan A, Joshi L, Kilcoyne M, Berbis MA, Canada FJ, Jimenez-Barbero J, Menendez M, Wilson SS, Bromme BA, Smith JG, van Raaij MJ J Gen Virol. 2018 Oct 2. doi: 10.1099/jgv.0.001145. PMID:30277856<ref>PMID:30277856</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5nc1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Murine adenovirus 2]]
	[[Category: Raaij, M J.van]]		[[Category: Raaij, M J.van]]
	[[Category: Singh, A K]]		[[Category: Singh, A K]]

OCA at 06:19, 14 March 2018

2018-03-14T06:19:28Z

←Older revision		Revision as of 06:19, 14 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5nc1 is ON HOLD~~	+	==Structure of the distal domain of mouse adenovirus 2 fibre bound to N-acetyl-glucosamine==
-		+	<StructureSection load='5nc1' size='340' side='right' caption='[[5nc1]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors: Singh, A.K., van Raaij, M.J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5nc1]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NC1 FirstGlance]. <br>
-	~~Description:~~ Structure of the distal domain of mouse adenovirus 2 fibre bound to N-acetyl-~~glycosamine~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5n83\|5n83]], [[5n8d\|5n8d]], [[5nbh\|5nbh]]</td></tr>
-	[[Category: ~~Van~~ Raaij, M.J]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nc1 OCA], [http://pdbe.org/5nc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nc1 RCSB], [http://www.ebi.ac.uk/pdbsum/5nc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nc1 ProSAT]</span></td></tr>
-	[[Category: Singh, A.K]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Raaij, M J.van]]
		+	[[Category: Singh, A K]]
		+	[[Category: 3-bladed propeller]]
		+	[[Category: Abcj-ghid topology]]
		+	[[Category: N-acetyl-glucosamine binding]]
		+	[[Category: Triple beta-spiral]]
		+	[[Category: Viral protein]]

OCA: Protected "5nc1" [edit=sysop:move=sysop]

2017-03-09T12:26:05Z

Protected "5nc1" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:26, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5nc1 is ON HOLD Authors: Singh, A.K., van Raaij, M.J. Description: Structure of the distal domain of mouse adenovirus 2 fibre bound to N-acetyl-gly...

2017-03-09T12:26:04Z

New page: '''Unreleased structure''' The entry 5nc1 is ON HOLD Authors: Singh, A.K., van Raaij, M.J. Description: Structure of the distal domain of mouse adenovirus 2 fibre bound to N-acetyl-gly...

New page

'''Unreleased structure'''

The entry 5nc1 is ON HOLD

Authors: Singh, A.K., van Raaij, M.J.

Description: Structure of the distal domain of mouse adenovirus 2 fibre bound to N-acetyl-glycosamine
[[Category: Unreleased Structures]]
[[Category: Van Raaij, M.J]]
[[Category: Singh, A.K]]