5ne3 - Revision history

OCA at 12:50, 15 November 2023

2023-11-15T12:50:22Z

←Older revision		Revision as of 12:50, 15 November 2023
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	<StructureSection load='5ne3' size='340' side='right'caption='[[5ne3]], [[Resolution\|resolution]] 1.35Å' scene=''>		<StructureSection load='5ne3' size='340' side='right'caption='[[5ne3]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ne3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NE3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NE3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ne3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stenotrophomonas_maltophilia_K279a Stenotrophomonas maltophilia K279a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NE3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NXL:(2S~~,~~5R)-1-FORMYL-5-~~[~~(SULFOOXY)AMINO~~]~~PIPERIDINE-2-CARBOXAMIDE'>NXL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.35Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Beta~~-~~lactamase Beta~~-~~lactamase~~]~~, with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.5.~~2~~.6 3.5.2.6]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NXL:(2S,5R)-1-FORMYL-5-[(SULFOOXY)AMINO]PIPERIDINE-2-CARBOXAMIDE'>NXL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ne3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ne3 OCA], [~~http~~://pdbe.org/5ne3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ne3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ne3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ne3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ne3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ne3 OCA], [https://pdbe.org/5ne3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ne3 RCSB], [https://www.ebi.ac.uk/pdbsum/5ne3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ne3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B2FRP5_STRMK B2FRP5_STRMK]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Calvopina, K~~]]	+	[[Category: Stenotrophomonas maltophilia K279a]]
-	[[Category: ~~Hinchliffe, P~~]]	+	[[Category: Calvopina K]]
-	[[Category: ~~Spencer, J~~]]	+	[[Category: Hinchliffe P]]
-	[[Category: ~~Avibactam]]~~	+	[[Category: Spencer J]]
-	~~[[Category: Carbapenemase]]~~	+
-	~~[[Category: Hydrolase-inhibitor complex]]~~	+
-	~~[[Category: Inhibitor~~]]	+

OCA at 12:03, 26 August 2020

2020-08-26T12:03:10Z

←Older revision		Revision as of 12:03, 26 August 2020
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	==L2 class A serine-beta-lactamase complexed with avibactam==		==L2 class A serine-beta-lactamase complexed with avibactam==
-	<StructureSection load='5ne3' size='340' side='right' caption='[[5ne3]], [[Resolution\|resolution]] 1.35Å' scene=''>	+	<StructureSection load='5ne3' size='340' side='right'caption='[[5ne3]], [[Resolution\|resolution]] 1.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ne3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NE3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NE3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ne3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NE3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NE3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NXL:(2S,5R)-1-FORMYL-5-[(SULFOOXY)AMINO]PIPERIDINE-2-CARBOXAMIDE'>NXL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NXL:(2S,5R)-1-FORMYL-5-[(SULFOOXY)AMINO]PIPERIDINE-2-CARBOXAMIDE'>NXL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ne3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ne3 OCA], [http://pdbe.org/5ne3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ne3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ne3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ne3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ne3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ne3 OCA], [http://pdbe.org/5ne3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ne3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ne3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ne3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5ne3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ne3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Calvopina, K]]		[[Category: Calvopina, K]]
	[[Category: Hinchliffe, P]]		[[Category: Hinchliffe, P]]

OCA at 04:32, 21 September 2017

2017-09-21T04:32:23Z

←Older revision		Revision as of 04:32, 21 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ne3 is ~~ON HOLD until Paper~~ Publication	+	==L2 class A serine-beta-lactamase complexed with avibactam==
		+	<StructureSection load='5ne3' size='340' side='right' caption='[[5ne3]], [[Resolution\|resolution]] 1.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ne3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NE3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NE3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NXL:(2S,5R)-1-FORMYL-5-[(SULFOOXY)AMINO]PIPERIDINE-2-CARBOXAMIDE'>NXL</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ne3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ne3 OCA], [http://pdbe.org/5ne3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ne3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ne3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ne3 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clavulanic acid and avibactam are clinically deployed serine beta-lactamase inhibitors, important as a defence against antibacterial resistance. Bicyclic boronates are recently discovered inhibitors of serine and some metallo beta-lactamases. Here we show that avibactam and a bicyclic boronate inhibit L2 (serine beta-lactamase) but not L1 (metallo beta-lactamase) from the extensively drug resistant human pathogen Stenotrophomonas maltophilia. X-ray crystallography revealed that both inhibitors bind L2 by covalent attachment to the nucleophilic serine. Both inhibitors reverse ceftazidime resistance in S. maltophilia because, unlike clavulanic acid, they do not induce L1 production. Ceftazidime/inhibitor resistant mutants hyper-produce L1, but retain aztreonam/inhibitor susceptibility because aztreonam is not an L1 substrate. Importantly, avibactam, but not the bicyclic boronate is deactivated by L1 at a low rate; the utility of avibactam might be compromised by mutations that increase this deactivation rate. These data rationalize the observed clinical efficacy of ceftazidime/avibactam plus aztreonam as combination therapy for S. maltophilia infections and confirm that aztreonam-like beta-lactams plus non-classical beta-lactamase inhibitors, particularly avibactam-like and bicyclic boronate compounds, have potential for treating infections caused by this most intractable of drug resistant pathogens. This article is protected by copyright. All rights reserved.

-	~~Authors: Hinchliffe, P~~., Calvopina, K., ~~Spencer~~, J.	+	Structural/mechanistic insights into the efficacy of non-classical beta-lactamase inhibitors against extensively drug resistant Stenotrophomonas maltophilia clinical isolates.,Calvopina K, Hinchliffe P, Brem J, Heesom KJ, Johnson S, Cain R, Lohans CT, Fishwick CWG, Schofield CJ, Spencer J, Avison MB Mol Microbiol. 2017 Sep 6. doi: 10.1111/mmi.13831. PMID:28876489<ref>PMID:28876489</ref>

-	~~Description: L2~~ class ~~A serine~~-~~beta~~-~~lactamase complexed with avibactam~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Spencer, J]]~~	+	<div class="pdbe-citations 5ne3" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Hinchliffe, P~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-lactamase]]
	[[Category: Calvopina, K]]		[[Category: Calvopina, K]]
		+	[[Category: Hinchliffe, P]]
		+	[[Category: Spencer, J]]
		+	[[Category: Avibactam]]
		+	[[Category: Carbapenemase]]
		+	[[Category: Hydrolase-inhibitor complex]]
		+	[[Category: Inhibitor]]

OCA at 09:56, 5 April 2017

2017-04-05T09:56:50Z

←Older revision		Revision as of 09:56, 5 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ne3 is ON HOLD	+	The entry 5ne3 is ON HOLD until Paper Publication

-	Authors: Hinchliffe, P., Calvopina, K, Spencer, J.	+	Authors: Hinchliffe, P., Calvopina, K., Spencer, J.

	Description: L2 class A serine-beta-lactamase complexed with avibactam		Description: L2 class A serine-beta-lactamase complexed with avibactam
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Calvopina, K,~~ Spencer, J]]	+	[[Category: Spencer, J]]
	[[Category: Hinchliffe, P]]		[[Category: Hinchliffe, P]]
		+	[[Category: Calvopina, K]]

OCA: Protected "5ne3" [edit=sysop:move=sysop]

2017-03-15T17:29:02Z

Protected "5ne3" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:29, 15 March 2017

OCA: New page: '''Unreleased structure''' The entry 5ne3 is ON HOLD Authors: Hinchliffe, P., Calvopina, K, Spencer, J. Description: L2 class A serine-beta-lactamase complexed with avibactam [[Categor...

2017-03-15T17:29:01Z

New page: '''Unreleased structure''' The entry 5ne3 is ON HOLD Authors: Hinchliffe, P., Calvopina, K, Spencer, J. Description: L2 class A serine-beta-lactamase complexed with avibactam [[Categor...

New page

'''Unreleased structure'''

The entry 5ne3 is ON HOLD

Authors: Hinchliffe, P., Calvopina, K, Spencer, J.

Description: L2 class A serine-beta-lactamase complexed with avibactam
[[Category: Unreleased Structures]]
[[Category: Calvopina, K, Spencer, J]]
[[Category: Hinchliffe, P]]