5nnt - Revision history

OCA at 13:13, 15 November 2023

2023-11-15T13:13:32Z

←Older revision		Revision as of 13:13, 15 November 2023
Line 3:		Line 3:
	<StructureSection load='5nnt' size='340' side='right'caption='[[5nnt]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='5nnt' size='340' side='right'caption='[[5nnt]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nnt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NNT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NNT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nnt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NNT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPV:DODECYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>DPV</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.209Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nnt OCA], [~~http~~://pdbe.org/5nnt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nnt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nnt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nnt ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPV:DODECYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>DPV</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nnt OCA], [https://pdbe.org/5nnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nnt RCSB], [https://www.ebi.ac.uk/pdbsum/5nnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nnt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CAMP_HUMAN CAMP_HUMAN]] Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.<ref>PMID:16637646</ref> <ref>PMID:18818205</ref>	+	[https://www.uniprot.org/uniprot/CAMP_HUMAN CAMP_HUMAN] Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.<ref>PMID:16637646</ref> <ref>PMID:18818205</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Sancho-Vaello, E]]	+	[[Category: Sancho-Vaello E]]
-	[[Category: Zeth, K]]	+	[[Category: Zeth K]]
-	~~[[Category: Amp]]~~	+
-	~~[[Category: Antimicrobial peptide]]~~	+
-	~~[[Category: Antimicrobial protein]]~~	+
-	~~[[Category: Ldao]]~~	+
-	~~[[Category: Ll-37]]~~	+
-	~~[[Category: Peptide-detergent complex~~]]	+

OCA at 06:45, 16 October 2019

2019-10-16T06:45:51Z

←Older revision		Revision as of 06:45, 16 October 2019
Line 1:		Line 1:

	==The dimeric structure of LL-37 crystallized in DPC==		==The dimeric structure of LL-37 crystallized in DPC==
-	<StructureSection load='5nnt' size='340' side='right' caption='[[5nnt]], [[Resolution\|resolution]] 2.21Å' scene=''>	+	<StructureSection load='5nnt' size='340' side='right'caption='[[5nnt]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5nnt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NNT FirstGlance]. <br>		<table><tr><td colspan='2'>[[5nnt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NNT FirstGlance]. <br>
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Sancho-Vaello, E]]		[[Category: Sancho-Vaello, E]]
	[[Category: Zeth, K]]		[[Category: Zeth, K]]

OCA at 18:22, 24 January 2018

2018-01-24T18:22:17Z

←Older revision		Revision as of 18:22, 24 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5nnt is ~~ON HOLD~~	+	==The dimeric structure of LL-37 crystallized in DPC==
		+	<StructureSection load='5nnt' size='340' side='right' caption='[[5nnt]], [[Resolution\|resolution]] 2.21Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nnt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NNT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NNT FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPV:DODECYL+2-(TRIMETHYLAMMONIO)ETHYL+PHOSPHATE'>DPV</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nnt OCA], [http://pdbe.org/5nnt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nnt RCSB], [http://www.ebi.ac.uk/pdbsum/5nnt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nnt ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CAMP_HUMAN CAMP_HUMAN]] Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.<ref>PMID:16637646</ref> <ref>PMID:18818205</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Antimicrobial peptides as part of the mammalian innate immune system target and remove major bacterial pathogens, often through irreversible damage of their cellular membranes. To explore the mechanism by which the important cathelicidin peptide LL-37 of the human innate immune system interacts with membranes, we performed biochemical, biophysical and structural studies. The crystal structure of LL-37 displays dimers of anti-parallel helices and the formation of amphipathic surfaces. Peptide-detergent interactions introduce remodeling of this structure after occupation of defined hydrophobic sites at the dimer interface. Furthermore, hydrophobic nests are shaped between dimer structures providing another scaffold enclosing detergents. Both scaffolds underline the potential of LL-37 to form defined peptide-lipid complexes in vivo. After adopting the activated peptide conformation LL-37 can polymerize and selectively extract bacterial lipids whereby the membrane is destabilized. The supramolecular fibril-like architectures formed in crystals can be reproduced in a peptide-lipid system after nanogold-labelled LL-37 interacted with lipid vesicles as followed by electron microscopy. We suggest that these supramolecular structures represent the LL-37-membrane active state. Collectively, our study provides new insights into the fascinating plasticity of LL-37 demonstrated at atomic resolution and opens the venue for LL-37-based molecules as novel antibiotics.

-	~~Authors: Zeth, K~~., Sancho-Vaello, E.	+	Structural remodeling and oligomerization of human cathelicidin on membranes suggest fibril-like structures as active species.,Sancho-Vaello E, Francois P, Bonetti EJ, Lilie H, Finger S, Gil-Ortiz F, Gil-Carton D, Zeth K Sci Rep. 2017 Nov 13;7(1):15371. doi: 10.1038/s41598-017-14206-1. PMID:29133814<ref>PMID:29133814</ref>

-	~~Description: The dimeric structure~~ of ~~LL-37 crystallized in DPC~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5nnt" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Sancho-Vaello, E]]		[[Category: Sancho-Vaello, E]]
	[[Category: Zeth, K]]		[[Category: Zeth, K]]
		+	[[Category: Amp]]
		+	[[Category: Antimicrobial peptide]]
		+	[[Category: Antimicrobial protein]]
		+	[[Category: Ldao]]
		+	[[Category: Ll-37]]
		+	[[Category: Peptide-detergent complex]]

OCA: Protected "5nnt" [edit=sysop:move=sysop]

2017-04-19T09:42:14Z

Protected "5nnt" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:42, 19 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nnt is ON HOLD Authors: Zeth, K., Sancho-Vaello, E. Description: The dimeric structure of LL-37 crystallized in DPC [[Category: Unreleased Structu...

2017-04-19T09:42:13Z

New page: '''Unreleased structure''' The entry 5nnt is ON HOLD Authors: Zeth, K., Sancho-Vaello, E. Description: The dimeric structure of LL-37 crystallized in DPC [[Category: Unreleased Structu...

New page

'''Unreleased structure'''

The entry 5nnt is ON HOLD

Authors: Zeth, K., Sancho-Vaello, E.

Description: The dimeric structure of LL-37 crystallized in DPC
[[Category: Unreleased Structures]]
[[Category: Sancho-Vaello, E]]
[[Category: Zeth, K]]