5nq6 - Revision history

OCA at 13:21, 15 November 2023

2023-11-15T13:21:52Z

←Older revision		Revision as of 13:21, 15 November 2023
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	<StructureSection load='5nq6' size='340' side='right'caption='[[5nq6]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5nq6' size='340' side='right'caption='[[5nq6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nq6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQ6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5NQ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nq6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NQ6 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5ft4\|5ft4]]~~, ~~[[5ft5\|5ft5]], [[5ft6\|5ft6]], [[5ft8\|5ft8]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5nq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nq6 OCA], [~~http~~://pdbe.org/5nq6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nq6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nq6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nq6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nq6 OCA], [https://pdbe.org/5nq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nq6 RCSB], [https://www.ebi.ac.uk/pdbsum/5nq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nq6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CSDE_ECOLI CSDE_ECOLI]] Stimulates the cysteine desulfurase activity of CsdA. Contains a cysteine residue (Cys-61) that acts to accept sulfur liberated via the desulfurase activity of CsdA. May be able to transfer sulfur to TcdA/CsdL. Seems to support the function of TcdA in the generation of cyclic threonylcarbamoyladenosine at position 37 (ct(6)A37) in tRNAs that read codons beginning with adenine. Does not appear to participate in Fe/S biogenesis.<ref>PMID:15901727</ref> <ref>PMID:20054882</ref> <ref>PMID:23242255</ref>	+	[https://www.uniprot.org/uniprot/CSDE_ECOLI CSDE_ECOLI] Stimulates the cysteine desulfurase activity of CsdA. Contains a cysteine residue (Cys-61) that acts to accept sulfur liberated via the desulfurase activity of CsdA. May be able to transfer sulfur to TcdA/CsdL. Seems to support the function of TcdA in the generation of cyclic threonylcarbamoyladenosine at position 37 (ct(6)A37) in tRNAs that read codons beginning with adenine. Does not appear to participate in Fe/S biogenesis.<ref>PMID:15901727</ref> <ref>PMID:20054882</ref> <ref>PMID:23242255</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aranda, J]]	+	[[Category: Aranda J]]
-	[[Category: Coll, M]]	+	[[Category: Coll M]]
-	[[Category: Fernandez~~, F J~~]]	+	[[Category: Fernandez FJ]]
-	[[Category: Garces, F]]	+	[[Category: Garces F]]
-	[[Category: Gomez, S]]	+	[[Category: Gomez S]]
-	[[Category: Lopez-Estepa, M]]	+	[[Category: Lopez-Estepa M]]
-	[[Category: Penya-Soler, E]]	+	[[Category: Penya-Soler E]]
-	[[Category: Vega~~, M C]]~~	+	[[Category: Vega MC]]
-	~~[[Category: L-cysteine desulfurase]]~~	+
-	~~[[Category: Sulfur trafficking]]~~	+
-	~~[[Category: Sulfur-acceptor protein]]~~	+
-	~~[[Category: Sulfur-binding protein]]~~	+
-	~~[[Category: Transpersulfuration~~]]	+

OCA at 12:10, 26 August 2020

2020-08-26T12:10:00Z

←Older revision		Revision as of 12:10, 26 August 2020
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	==Crystal structure of the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE from Escherichia coli at 2.40 Angstrom Resolution==		==Crystal structure of the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE from Escherichia coli at 2.40 Angstrom Resolution==
-	<StructureSection load='5nq6' size='340' side='right' caption='[[5nq6]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5nq6' size='340' side='right'caption='[[5nq6]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nq6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NQ6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nq6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NQ6 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ft4\|5ft4]], [[5ft5\|5ft5]], [[5ft6\|5ft6]], [[5ft8\|5ft8]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ft4\|5ft4]], [[5ft5\|5ft5]], [[5ft6\|5ft6]], [[5ft8\|5ft8]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nq6 OCA], [http://pdbe.org/5nq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nq6 RCSB], [http://www.ebi.ac.uk/pdbsum/5nq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nq6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nq6 OCA], [http://pdbe.org/5nq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nq6 RCSB], [http://www.ebi.ac.uk/pdbsum/5nq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nq6 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Aranda, J]]		[[Category: Aranda, J]]
	[[Category: Coll, M]]		[[Category: Coll, M]]

OCA at 06:29, 28 March 2018

2018-03-28T06:29:14Z

←Older revision		Revision as of 06:29, 28 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nq6 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE from Escherichia coli at 2.40 Angstrom Resolution==
		+	<StructureSection load='5nq6' size='340' side='right' caption='[[5nq6]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5nq6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NQ6 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5ft4\|5ft4]], [[5ft5\|5ft5]], [[5ft6\|5ft6]], [[5ft8\|5ft8]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nq6 OCA], [http://pdbe.org/5nq6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nq6 RCSB], [http://www.ebi.ac.uk/pdbsum/5nq6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nq6 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CSDE_ECOLI CSDE_ECOLI]] Stimulates the cysteine desulfurase activity of CsdA. Contains a cysteine residue (Cys-61) that acts to accept sulfur liberated via the desulfurase activity of CsdA. May be able to transfer sulfur to TcdA/CsdL. Seems to support the function of TcdA in the generation of cyclic threonylcarbamoyladenosine at position 37 (ct(6)A37) in tRNAs that read codons beginning with adenine. Does not appear to participate in Fe/S biogenesis.<ref>PMID:15901727</ref> <ref>PMID:20054882</ref> <ref>PMID:23242255</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sulfur trafficking in living organisms relies on transpersulfuration reactions consisting in the enzyme-catalyzed transfer of S atoms via activated persulfidic S across protein-protein interfaces. The recent elucidation of the mechanistic basis for transpersulfuration in the CsdA-CsdE model system has paved the way for a better understanding of its role under oxidative stress. Herein we present the crystal structure of the oxidized, inactivated CsdE dimer at 2.4 A resolution. The structure sheds light into the activation of the Cys61 nucleophile on its way from a solvent-secluded position in free CsdE to a fully extended conformation in the persulfurated CsdA-CsdE complex. Molecular dynamics simulations of available CsdE structures allow to delineate the sequence of conformational changes underwent by CsdE and to pinpoint the key role played by the deprotonation of the Cys61 thiol. The low-energy subunit orientation in the disulfide-bridged CsdE dimer demonstrates the likely physiologic relevance of this oxidative dead-end form of CsdE, suggesting that CsdE could act as a redox sensor in vivo.

-	~~Authors~~:	+	Insights into the inhibited form of the redox-sensitive SufE-like sulfur acceptor CsdE.,Pena-Soler E, Aranda J, Lopez-Estepa M, Gomez S, Garces F, Coll M, Fernandez FJ, Tunon I, Vega MC PLoS One. 2017 Oct 18;12(10):e0186286. doi: 10.1371/journal.pone.0186286., eCollection 2017. PMID:29045454<ref>PMID:29045454</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5nq6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aranda, J]]
		+	[[Category: Coll, M]]
		+	[[Category: Fernandez, F J]]
		+	[[Category: Garces, F]]
		+	[[Category: Gomez, S]]
		+	[[Category: Lopez-Estepa, M]]
		+	[[Category: Penya-Soler, E]]
		+	[[Category: Vega, M C]]
		+	[[Category: L-cysteine desulfurase]]
		+	[[Category: Sulfur trafficking]]
		+	[[Category: Sulfur-acceptor protein]]
		+	[[Category: Sulfur-binding protein]]
		+	[[Category: Transpersulfuration]]

OCA at 09:34, 4 May 2017

2017-05-04T09:34:40Z

←Older revision		Revision as of 09:34, 4 May 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5nq6 is ON HOLD	+	The entry 5nq6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5nq6" [edit=sysop:move=sysop]

2017-04-27T10:46:47Z

Protected "5nq6" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:46, 27 April 2017

OCA: New page: '''Unreleased structure''' The entry 5nq6 is ON HOLD Authors: Description: Category: Unreleased Structures

2017-04-27T10:46:46Z

New page: '''Unreleased structure''' The entry 5nq6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5nq6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]