5nun - Revision history

OCA at 11:50, 1 February 2024

2024-02-01T11:50:43Z

←Older revision		Revision as of 11:50, 1 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5nun]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUN FirstGlance]. <br>		<table><tr><td colspan='2'>[[5nun]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nun OCA], [https://pdbe.org/5nun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nun RCSB], [https://www.ebi.ac.uk/pdbsum/5nun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nun ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nun OCA], [https://pdbe.org/5nun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nun RCSB], [https://www.ebi.ac.uk/pdbsum/5nun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nun ProSAT]</span></td></tr>
	</table>		</table>

OCA at 08:25, 7 December 2022

2022-12-07T08:25:24Z

←Older revision		Revision as of 08:25, 7 December 2022
Line 1:		Line 1:

	==Engineered beta-lactoglobulin: variant F105L-L39A-M107F in complex with chlorpromazine (LG-LAF-CLP)==		==Engineered beta-lactoglobulin: variant F105L-L39A-M107F in complex with chlorpromazine (LG-LAF-CLP)==
-	<StructureSection load='5nun' size='340' side='right' caption='[[5nun]], [[Resolution\|resolution]] 1.95Å' scene=''>	+	<StructureSection load='5nun' size='340' side='right'caption='[[5nun]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nun]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NUN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nun]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NUN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nun OCA], [https://pdbe.org/5nun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nun RCSB], [https://www.ebi.ac.uk/pdbsum/5nun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nun ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nun OCA], [~~http~~://pdbe.org/5nun PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nun RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nun PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nun ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.	+	[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5nun" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5nun" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-lactoglobulin 3D structures\|Beta-lactoglobulin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
-	[[Category: Bonarek, P]]	+	[[Category: Large Structures]]
-	[[Category: Lazinskia, I]]	+	[[Category: Bonarek P]]
-	[[Category: Lewinski, K]]	+	[[Category: Lazinskia I]]
-	[[Category: Loch~~, J I~~]]	+	[[Category: Lewinski K]]
-	[[Category: Szydlowska, J]]	+	[[Category: Loch JI]]
-	[[Category: Towrzydlo, M]]	+	[[Category: Szydlowska J]]
-	~~[[Category: Beta-lactoglobulin]]~~	+	[[Category: Towrzydlo M]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Lipocalin]]~~	+
-	~~[[Category: Mutation]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 06:27, 18 April 2018

2018-04-18T06:27:24Z

←Older revision		Revision as of 06:27, 18 April 2018
Line 3:		Line 3:
	<StructureSection load='5nun' size='340' side='right' caption='[[5nun]], [[Resolution\|resolution]] 1.95Å' scene=''>		<StructureSection load='5nun' size='340' side='right' caption='[[5nun]], [[Resolution\|resolution]] 1.95Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nun]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nun]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">LGB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nun OCA], [http://pdbe.org/5nun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nun RCSB], [http://www.ebi.ac.uk/pdbsum/5nun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nun ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nun OCA], [http://pdbe.org/5nun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nun RCSB], [http://www.ebi.ac.uk/pdbsum/5nun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nun ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.		[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chlorpromazine (CPZ) is a phenothiazine acting as dopamine antagonist. Aside from application in schizophrenia therapy, chlorpromazine is found to be a putative inhibitor of proteins involved in cancers, heritable autism disorder and prion diseases. Four new beta-lactoglobulin variants with double or triple substitutions: I56F/L39A, F105L/L39A, I56F/L39A/M107F or F105L/L39A/M107F changing the shape of the binding pocket were produced and their chlorpromazine binding properties have been investigated by X-ray crystallography, circular dichroism, isothermal titration calorimetry and thermophoresis. The CD spectra and crystal structures revealed that mutations do not affect the protein overall structure but in comparison to WT protein, variants possessing I56F substitution had lower stability while mutation F105L increased melting temperature of the protein. The new variants showed affinity to chlorpromazine in the range 4.2-15.4x10(3)M(-1). The CD spectra and crystal structures revealed complementarity of the binding pocket shape, to only one chlorpromazine chiral conformer. The (aR)-CPZ was bonded to variants containing I56F substitution while variants with F105L substitution preferred (aS)-CPZ.
		+
		+	The engineered beta-lactoglobulin with complementarity to the chlorpromazine chiral conformers.,Loch JI, Bonarek P, Tworzydlo M, Lazinska I, Szydlowska J, Lipowska J, Rzesikowska K, Lewinski K Int J Biol Macromol. 2018 Mar 16;114:85-96. doi: 10.1016/j.ijbiomac.2018.03.074. PMID:29555509<ref>PMID:29555509</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5nun" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bovin]]
	[[Category: Bonarek, P]]		[[Category: Bonarek, P]]
	[[Category: Lazinskia, I]]		[[Category: Lazinskia, I]]

OCA at 05:51, 4 April 2018

2018-04-04T05:51:50Z

←Older revision		Revision as of 05:51, 4 April 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5nun is ON HOLD until Paper Publication~~	+	==Engineered beta-lactoglobulin: variant F105L-L39A-M107F in complex with chlorpromazine (LG-LAF-CLP)==
-		+	<StructureSection load='5nun' size='340' side='right' caption='[[5nun]], [[Resolution\|resolution]] 1.95Å' scene=''>
-	~~Authors: Loch, J.I., Bonarek, P., Towrzydlo, M., Lazinskia, I., Szydlowska, J., Lewinski, K.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5nun]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NUN FirstGlance]. <br>
-	~~Description:~~ Engineered beta-lactoglobulin: variant F105L-L39A-M107F in complex with chlorpromazine (LG-LAF-CLP)	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=Z80:3-(2-CHLORO-10H-PHENOTHIAZIN-10-YL)-N,N-DIMETHYLPROPAN-1-AMINE'>Z80</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nun OCA], [http://pdbe.org/5nun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nun RCSB], [http://www.ebi.ac.uk/pdbsum/5nun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nun ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN]] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bonarek, P]]
	[[Category: Lazinskia, I]]		[[Category: Lazinskia, I]]
-	[[Category: Loch, J.I]]
	[[Category: Lewinski, K]]		[[Category: Lewinski, K]]
-	[[Category: ~~Bonarek~~, P]]	+	[[Category: Loch, J I]]
	[[Category: Szydlowska, J]]		[[Category: Szydlowska, J]]
	[[Category: Towrzydlo, M]]		[[Category: Towrzydlo, M]]
		+	[[Category: Beta-lactoglobulin]]
		+	[[Category: Complex]]
		+	[[Category: Lipocalin]]
		+	[[Category: Mutation]]
		+	[[Category: Transport protein]]

OCA at 11:44, 27 September 2017

2017-09-27T11:44:23Z

←Older revision		Revision as of 11:44, 27 September 2017
Line 3:		Line 3:
	The entry 5nun is ON HOLD until Paper Publication		The entry 5nun is ON HOLD until Paper Publication

-	Authors: Loch, J.I, Bonarek, P., Towrzydlo, M., Lazinskia, I., Szydlowska, J., Lewinski, K.	+	Authors: Loch, J.I., Bonarek, P., Towrzydlo, M., Lazinskia, I., Szydlowska, J., Lewinski, K.

	Description: Engineered beta-lactoglobulin: variant F105L-L39A-M107F in complex with chlorpromazine (LG-LAF-CLP)		Description: Engineered beta-lactoglobulin: variant F105L-L39A-M107F in complex with chlorpromazine (LG-LAF-CLP)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Loch, J.I, Bonarek, P]]
	[[Category: Lazinskia, I]]		[[Category: Lazinskia, I]]
		+	[[Category: Loch, J.I]]
	[[Category: Lewinski, K]]		[[Category: Lewinski, K]]
		+	[[Category: Bonarek, P]]
	[[Category: Szydlowska, J]]		[[Category: Szydlowska, J]]
	[[Category: Towrzydlo, M]]		[[Category: Towrzydlo, M]]

OCA at 12:17, 17 May 2017

2017-05-17T12:17:46Z

←Older revision		Revision as of 12:17, 17 May 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5nun is ON HOLD	+	The entry 5nun is ON HOLD until Paper Publication

	Authors: Loch, J.I, Bonarek, P., Towrzydlo, M., Lazinskia, I., Szydlowska, J., Lewinski, K.		Authors: Loch, J.I, Bonarek, P., Towrzydlo, M., Lazinskia, I., Szydlowska, J., Lewinski, K.

OCA: Protected "5nun" [edit=sysop:move=sysop]

2017-05-10T09:22:15Z

Protected "5nun" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:22, 10 May 2017

OCA: New page: '''Unreleased structure''' The entry 5nun is ON HOLD Authors: Loch, J.I, Bonarek, P., Towrzydlo, M., Lazinskia, I., Szydlowska, J., Lewinski, K. Description: Engineered beta-lactoglobu...

2017-05-10T09:22:14Z

New page: '''Unreleased structure''' The entry 5nun is ON HOLD Authors: Loch, J.I, Bonarek, P., Towrzydlo, M., Lazinskia, I., Szydlowska, J., Lewinski, K. Description: Engineered beta-lactoglobu...

New page

'''Unreleased structure'''

The entry 5nun is ON HOLD

Authors: Loch, J.I, Bonarek, P., Towrzydlo, M., Lazinskia, I., Szydlowska, J., Lewinski, K.

Description: Engineered beta-lactoglobulin: variant F105L-L39A-M107F in complex with chlorpromazine (LG-LAF-CLP)
[[Category: Unreleased Structures]]
[[Category: Loch, J.I, Bonarek, P]]
[[Category: Lazinskia, I]]
[[Category: Lewinski, K]]
[[Category: Szydlowska, J]]
[[Category: Towrzydlo, M]]