5nv5 - Revision history

OCA at 12:09, 22 November 2023

2023-11-22T12:09:59Z

←Older revision		Revision as of 12:09, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5nv5' size='340' side='right'caption='[[5nv5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5nv5' size='340' side='right'caption='[[5nv5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nv5]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NV5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5NV5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nv5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NV5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NV5 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>D350_01176 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1351~~ "~~Enterococcus proteiformis~~" ~~Thiercelin and Jouhaud 1903~~])</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5nv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nv5 OCA], [~~http~~://pdbe.org/5nv5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5nv5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5nv5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5nv5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nv5 OCA], [https://pdbe.org/5nv5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nv5 RCSB], [https://www.ebi.ac.uk/pdbsum/5nv5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nv5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/U6S0Y1_ENTFL U6S0Y1_ENTFL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5nv5" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5nv5" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fic protein 3D structures\|Fic protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterococcus ~~proteiformis thiercelin and jouhaud 1903~~]]	+	[[Category: Enterococcus faecalis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cherfils, J]]	+	[[Category: Cherfils J]]
-	[[Category: Veyron, S]]	+	[[Category: Veyron S]]
-	~~[[Category: Toxin~~]]	+

OCA at 08:13, 20 March 2019

2019-03-20T08:13:59Z

←Older revision		Revision as of 08:13, 20 March 2019
Line 1:		Line 1:

	==Enterococcus faecalis FIC protein==		==Enterococcus faecalis FIC protein==
-	<StructureSection load='5nv5' size='340' side='right' caption='[[5nv5]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='5nv5' size='340' side='right'caption='[[5nv5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5nv5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NV5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5nv5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NV5 FirstGlance]. <br>
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nv5 OCA], [http://pdbe.org/5nv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nv5 RCSB], [http://www.ebi.ac.uk/pdbsum/5nv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nv5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nv5 OCA], [http://pdbe.org/5nv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nv5 RCSB], [http://www.ebi.ac.uk/pdbsum/5nv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nv5 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	FIC proteins regulate molecular processes from bacteria to humans by catalyzing post-translational modifications (PTM), the most frequent being the addition of AMP or AMPylation. In many AMPylating FIC proteins, a structurally conserved glutamate represses AMPylation and, in mammalian FICD, also supports deAMPylation of BiP/GRP78, a key chaperone of the unfolded protein response. Currently, a direct signal regulating these FIC proteins has not been identified. Here, we use X-ray crystallography and in vitro PTM assays to address this question. We discover that Enterococcus faecalis FIC (EfFIC) catalyzes both AMPylation and deAMPylation and that the glutamate implements a multi-position metal switch whereby Mg(2+) and Ca(2+) control AMPylation and deAMPylation differentially without a conformational change. Remarkably, Ca(2+) concentration also tunes deAMPylation of BiP by human FICD. Our results suggest that the conserved glutamate is a signature of AMPylation/deAMPylation FIC bifunctionality and identify metal ions as diffusible signals that regulate such FIC proteins directly.
		+
		+	A Ca(2+)-regulated deAMPylation switch in human and bacterial FIC proteins.,Veyron S, Oliva G, Rolando M, Buchrieser C, Peyroche G, Cherfils J Nat Commun. 2019 Mar 8;10(1):1142. doi: 10.1038/s41467-019-09023-1. PMID:30850593<ref>PMID:30850593</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5nv5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]		[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
		+	[[Category: Large Structures]]
	[[Category: Cherfils, J]]		[[Category: Cherfils, J]]
	[[Category: Veyron, S]]		[[Category: Veyron, S]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 09:40, 13 February 2019

2019-02-13T09:40:30Z

←Older revision		Revision as of 09:40, 13 February 2019
Line 1:		Line 1:

-	==~~Wild-type toxin from~~ Enterococcus faecalis==	+	==Enterococcus faecalis FIC protein==
	<StructureSection load='5nv5' size='340' side='right' caption='[[5nv5]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='5nv5' size='340' side='right' caption='[[5nv5]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5nv5]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NV5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5nv5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NV5 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nv5 OCA], [http://pdbe.org/5nv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nv5 RCSB], [http://www.ebi.ac.uk/pdbsum/5nv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nv5 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">D350_01176 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nv5 OCA], [http://pdbe.org/5nv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nv5 RCSB], [http://www.ebi.ac.uk/pdbsum/5nv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nv5 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
	[[Category: Cherfils, J]]		[[Category: Cherfils, J]]
	[[Category: Veyron, S]]		[[Category: Veyron, S]]
	[[Category: Toxin]]		[[Category: Toxin]]

OCA at 05:24, 30 May 2018

2018-05-30T05:24:54Z

←Older revision		Revision as of 05:24, 30 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5nv5 ~~is ON HOLD~~	+	==Wild-type toxin from Enterococcus faecalis==
-		+	<StructureSection load='5nv5' size='340' side='right' caption='[[5nv5]], [[Resolution\|resolution]] 2.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5nv5]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NV5 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nv5 OCA], [http://pdbe.org/5nv5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nv5 RCSB], [http://www.ebi.ac.uk/pdbsum/5nv5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nv5 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cherfils, J]]
		+	[[Category: Veyron, S]]
		+	[[Category: Toxin]]

OCA: Protected "5nv5" [edit=sysop:move=sysop]

2017-05-10T09:26:58Z

Protected "5nv5" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:26, 10 May 2017

OCA: New page: '''Unreleased structure''' The entry 5nv5 is ON HOLD Authors: Description: Category: Unreleased Structures

2017-05-10T09:26:57Z

New page: '''Unreleased structure''' The entry 5nv5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5nv5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]