5o7s - Revision history

OCA at 19:10, 29 November 2023

2023-11-29T19:10:45Z

←Older revision		Revision as of 19:10, 29 November 2023
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	<StructureSection load='5o7s' size='340' side='right'caption='[[5o7s]], [[Resolution\|resolution]] 2.02Å' scene=''>		<StructureSection load='5o7s' size='340' side='right'caption='[[5o7s]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o7s]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Diocu Diocu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5O7S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o7s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O7S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.02Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5o7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7s OCA], [~~http~~://pdbe.org/5o7s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5o7s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5o7s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7s ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7s OCA], [https://pdbe.org/5o7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o7s RCSB], [https://www.ebi.ac.uk/pdbsum/5o7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.	+	[https://www.uniprot.org/uniprot/MONA_DIOCU MONA_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Diocu~~]]	+	[[Category: Dioscoreophyllum cumminsii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Merlino, A]]	+	[[Category: Merlino A]]
-	[[Category: Pica, A]]	+	[[Category: Pica A]]
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Sweet protein]]~~	+
-	~~[[Category: Sweetener~~]]	+

OCA at 12:18, 26 August 2020

2020-08-26T12:18:45Z

←Older revision		Revision as of 12:18, 26 August 2020
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	==Crystal structure of a single chain monellin mutant (Y65R) pH 8.3==		==Crystal structure of a single chain monellin mutant (Y65R) pH 8.3==
-	<StructureSection load='5o7s' size='340' side='right' caption='[[5o7s]], [[Resolution\|resolution]] 2.02Å' scene=''>	+	<StructureSection load='5o7s' size='340' side='right'caption='[[5o7s]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o7s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7S OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5O7S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o7s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5O7S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5o7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7s OCA], [http://pdbe.org/5o7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7s RCSB], [http://www.ebi.ac.uk/pdbsum/5o7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7s ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5o7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7s OCA], [http://pdbe.org/5o7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7s RCSB], [http://www.ebi.ac.uk/pdbsum/5o7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Diocu]]		[[Category: Diocu]]
		+	[[Category: Large Structures]]
	[[Category: Merlino, A]]		[[Category: Merlino, A]]
	[[Category: Pica, A]]		[[Category: Pica, A]]

OCA at 06:41, 7 February 2018

2018-02-07T06:41:26Z

←Older revision		Revision as of 06:41, 7 February 2018
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	<StructureSection load='5o7s' size='340' side='right' caption='[[5o7s]], [[Resolution\|resolution]] 2.02Å' scene=''>		<StructureSection load='5o7s' size='340' side='right' caption='[[5o7s]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5o7s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5o7s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7s OCA], [http://pdbe.org/5o7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7s RCSB], [http://www.ebi.ac.uk/pdbsum/5o7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7s OCA], [http://pdbe.org/5o7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7s RCSB], [http://www.ebi.ac.uk/pdbsum/5o7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7s ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Diocu]]
	[[Category: Merlino, A]]		[[Category: Merlino, A]]
	[[Category: Pica, A]]		[[Category: Pica, A]]

OCA at 08:21, 10 January 2018

2018-01-10T08:21:53Z

←Older revision		Revision as of 08:21, 10 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5o7s is ~~ON HOLD~~	+	==Crystal structure of a single chain monellin mutant (Y65R) pH 8.3==
		+	<StructureSection load='5o7s' size='340' side='right' caption='[[5o7s]], [[Resolution\|resolution]] 2.02Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5o7s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5O7S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5o7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7s OCA], [http://pdbe.org/5o7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5o7s RCSB], [http://www.ebi.ac.uk/pdbsum/5o7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7s ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU]] Taste-modifying protein; intensely sweet-tasting protein.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: MNEI and its variant Y65R-MNEI are sweet proteins with potential applications as sweeteners in food industry. Also, they are often used as model systems for folding and aggregation studies. METHODS: X-ray crystallography was used to structurally characterize Y65R-MNEI at five different pHs, while circular dichroism and fluorescence spectroscopy their thermal and chemical stability. ThT assay and AFM were used for studying the kinetics of aggregation and morphology of the aggregates. RESULTS: Crystal structures of Y65R-MNEI revealed the existence of a dimer in the asymmetric unit, which, depending on the pH, assumes either an open or a closed conformation. The pH dramatically affects kinetics of formation and morphology of the aggregates: both MNEI and Y65R-MNEI form fibrils at acidic pH while amorphous aggregates are observed at neutral pH. CONCLUSIONS: The mutation Y65R induces structural modifications at the C-terminal region of the protein, which account for the decreased stability of the mutant when compared to MNEI. Furthermore, the pH-dependent conformation of the Y65R-MNEI dimer may explain the different type of aggregates formed as a function of pH. General Significance The investigation of the structural bases of aggregation gets us closer to the possibility of controlling such process, either by tuning the physicochemical environmental parameters or by site directed mutagenesis. This knowledge is helpful to expand the range of stability of proteins with potential industrial applications, such as MNEI and its mutant Y65R-MNEI, which should ideally preserve their structure and soluble state through a wide array of conditions. Classification structure-function relationship, protein stability, protein aggregation.

-	~~Authors~~: Pica, A., ~~Merlino~~, A.	+	pH driven fibrillar aggregation of the super-sweet protein Y65R-MNEI: a step-by-step structural analysis.,Pica A, Leone S, Di Girolamo R, Donnarumma F, Emendato A, Rega MF, Merlino A, Picone D Biochim Biophys Acta. 2017 Dec 27. pii: S0304-4165(17)30400-2. doi:, 10.1016/j.bbagen.2017.12.012. PMID:29288772<ref>PMID:29288772</ref>

-	~~Description: Crystal structure of~~ a ~~single chain monellin mutant (Y65R) pH 8~~.3	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5o7s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Merlino, A]]		[[Category: Merlino, A]]
	[[Category: Pica, A]]		[[Category: Pica, A]]
		+	[[Category: Plant protein]]
		+	[[Category: Sweet protein]]
		+	[[Category: Sweetener]]

OCA: Protected "5o7s" [edit=sysop:move=sysop]

2017-09-27T12:07:20Z

Protected "5o7s" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:07, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5o7s is ON HOLD Authors: Pica, A., Merlino, A. Description: Crystal structure of a single chain monellin mutant (Y65R) pH 8.3 [[Category: Unrelease...

2017-09-27T12:07:19Z

New page: '''Unreleased structure''' The entry 5o7s is ON HOLD Authors: Pica, A., Merlino, A. Description: Crystal structure of a single chain monellin mutant (Y65R) pH 8.3 [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 5o7s is ON HOLD

Authors: Pica, A., Merlino, A.

Description: Crystal structure of a single chain monellin mutant (Y65R) pH 8.3
[[Category: Unreleased Structures]]
[[Category: Merlino, A]]
[[Category: Pica, A]]