5ohu - Revision history

OCA at 16:50, 13 December 2023

2023-12-13T16:50:45Z

←Older revision		Revision as of 16:50, 13 December 2023
Line 1:		Line 1:

	==The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa==		==The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa==
-	<StructureSection load='5ohu' size='340' side='right' caption='[[5ohu]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='5ohu' size='340' side='right'caption='[[5ohu]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ohu]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OHU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OHU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ohu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OHU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OHU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=PO4:PHOSPHATE+ION'>PO4</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>slt, AOY09_04369, PAMH19_2049 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~287~~ "~~Bacillus aeruginosus~~" ~~(Schroeter 1872) Trevisan 1885])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ohu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ohu OCA], [~~http~~://pdbe.org/5ohu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ohu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ohu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ohu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ohu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ohu OCA], [https://pdbe.org/5ohu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ohu RCSB], [https://www.ebi.ac.uk/pdbsum/5ohu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ohu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9HZI6_PSEAE Q9HZI6_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Batuecas, M T~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Dominguez-Gil, T~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Hermoso, J A~~]]	+	[[Category: Batuecas MT]]
-	[[Category: ~~Lyase~~]]	+	[[Category: Dominguez-Gil T]]
-	[[Category: ~~Lytic transglycosylase~~]]	+	[[Category: Hermoso JA]]

OCA at 08:24, 2 May 2018

2018-05-02T08:24:36Z

←Older revision		Revision as of 08:24, 2 May 2018
Line 3:		Line 3:
	<StructureSection load='5ohu' size='340' side='right' caption='[[5ohu]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='5ohu' size='340' side='right' caption='[[5ohu]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ohu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OHU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ohu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OHU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">slt, AOY09_04369, PAMH19_2049 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ohu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ohu OCA], [http://pdbe.org/5ohu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ohu RCSB], [http://www.ebi.ac.uk/pdbsum/5ohu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ohu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ohu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ohu OCA], [http://pdbe.org/5ohu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ohu RCSB], [http://www.ebi.ac.uk/pdbsum/5ohu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ohu ProSAT]</span></td></tr>
	</table>		</table>

OCA at 05:36, 18 April 2018

2018-04-18T05:36:58Z

←Older revision		Revision as of 05:36, 18 April 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5ohu is ~~ON HOLD until Paper~~ Publication	+	==The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa==
		+	<StructureSection load='5ohu' size='340' side='right' caption='[[5ohu]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ohu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OHU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ohu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ohu OCA], [http://pdbe.org/5ohu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ohu RCSB], [http://www.ebi.ac.uk/pdbsum/5ohu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ohu ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-Lactam antibiotics inhibit cell-wall transpeptidases, preventing the peptidoglycan, the major constituent of the bacterial cell wall, from cross-linking. This causes accumulation of long non-cross-linked strands of peptidoglycan, which leads to bacterial death. Pseudomonas aeruginosa, a nefarious bacterial pathogen, attempts to repair this aberrantly formed peptidoglycan by the function of the lytic transglycosylase Slt. We document in this report that Slt turns over the peptidoglycan by both exolytic and endolytic reactions, which cause glycosidic bond scission from a terminus or in the middle of the peptidoglycan, respectively. These reactions were characterized with complex synthetic peptidoglycan fragments that ranged in size from tetrasaccharides to octasaccharides. The X-ray structure of the wild-type apo Slt revealed it to be a doughnut-shaped protein. In a series of six additional X-ray crystal structures, we provide insights with authentic substrates into how Slt is enabled for catalysis for both the endolytic and exolytic reactions. The substrate for the exolytic reaction binds Slt in a canonical arrangement and reveals how both the glycan chain and the peptide stems are recognized by the Slt. We document that the apo enzyme does not have a fully formed active site for the endolytic reaction. However, binding of the peptidoglycan at the existing subsites within the catalytic domain causes a conformational change in the protein that assembles the surface for binding of a more expansive peptidoglycan between the catalytic domain and an adjacent domain. The complexes of Slt with synthetic peptidoglycan substrates provide an unprecedented snapshot of the endolytic reaction.

-	~~Authors~~:	+	Exolytic and endolytic turnover of peptidoglycan by lytic transglycosylase Slt of Pseudomonas aeruginosa.,Lee M, Batuecas MT, Tomoshige S, Dominguez-Gil T, Mahasenan KV, Dik DA, Hesek D, Millan C, Uson I, Lastochkin E, Hermoso JA, Mobashery S Proc Natl Acad Sci U S A. 2018 Apr 9. pii: 1801298115. doi:, 10.1073/pnas.1801298115. PMID:29632171<ref>PMID:29632171</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5ohu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Batuecas, M T]]
		+	[[Category: Dominguez-Gil, T]]
		+	[[Category: Hermoso, J A]]
		+	[[Category: Lyase]]
		+	[[Category: Lytic transglycosylase]]

OCA at 04:55, 14 March 2018

2018-03-14T04:55:29Z

←Older revision		Revision as of 04:55, 14 March 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ohu is ON HOLD	+	The entry 5ohu is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5ohu" [edit=sysop:move=sysop]

2017-09-27T12:27:00Z

Protected "5ohu" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:27, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5ohu is ON HOLD Authors: Description: Category: Unreleased Structures

2017-09-27T12:26:59Z

New page: '''Unreleased structure''' The entry 5ohu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5ohu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]