5ojv - Revision history

OCA at 16:52, 13 December 2023

2023-12-13T16:52:27Z

←Older revision		Revision as of 16:52, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='5ojv' size='340' side='right'caption='[[5ojv]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='5ojv' size='340' side='right'caption='[[5ojv]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ojv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mychd Mychd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ojv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_hassiacum_DSM_44199 Mycolicibacterium hassiacum DSM 44199]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OJV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2M8:2-O-ALPHA-MANNOSYL-D-GLYCERATE'>2M8</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.062Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C731_0006 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1122247 MYCHD])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2M8:2-O-ALPHA-MANNOSYL-D-GLYCERATE'>2M8</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ojv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojv OCA], [~~http~~://pdbe.org/5ojv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ojv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ojv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ojv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojv OCA], [https://pdbe.org/5ojv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ojv RCSB], [https://www.ebi.ac.uk/pdbsum/5ojv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GGH_MYCHD GGH_MYCHD] Catalyzes the hydrolysis of glucosylglycerate (GG) to glycerate and glucose (PubMed:25341489, PubMed:31316802). Involved in recovery from nitrogen starvation by promoting the rapid mobilization of the glucosylglycerate that accumulates under these conditions (PubMed:25341489). Can also hydrolyze mannosylglycerate (MG), with tenfold lower efficiency (PubMed:31316802).<ref>PMID:25341489</ref> <ref>PMID:31316802</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mychd~~]]	+	[[Category: Mycolicibacterium hassiacum DSM 44199]]
-	[[Category: Cereija~~, T B~~]]	+	[[Category: Cereija TB]]
-	[[Category: Macedo-Ribeiro, S]]	+	[[Category: Macedo-Ribeiro S]]
-	[[Category: Pereira~~, P J.B]]~~	+	[[Category: Pereira PJB]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mycobacterium~~]]	+

OCA at 06:40, 31 July 2019

2019-07-31T06:40:18Z

←Older revision		Revision as of 06:40, 31 July 2019
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ojv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojv OCA], [http://pdbe.org/5ojv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ojv RCSB], [http://www.ebi.ac.uk/pdbsum/5ojv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ojv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojv OCA], [http://pdbe.org/5ojv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ojv RCSB], [http://www.ebi.ac.uk/pdbsum/5ojv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojv ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria are challenged to adapt to environmental variations in order to survive. Under nutritional stress, several bacteria are able to slow down their metabolism into a nonreplicating state and wait for favourable conditions. It is almost universal that bacteria accumulate carbon stores to survive during this nonreplicating state and to fuel rapid proliferation when the growth-limiting stress disappears. Mycobacteria are exceedingly successful in their ability to become dormant under harsh circumstances and to be able to resume growth when conditions are favourable. Rapidly growing mycobacteria accumulate glucosylglycerate under nitrogen-limiting conditions and quickly mobilize it when nitrogen availability is restored. The depletion of intracellular glucosyl-glycerate levels in Mycolicibacterium hassiacum (basonym Mycobacterium hassiacum) was associated with the up-regulation of the gene coding for glucosylglycerate hydrolase (GgH), an enzyme that is able to hydrolyse glucosylglycerate to glycerate and glucose, a source of readily available energy. Highly conserved among unrelated phyla, GgH is likely to be involved in bacterial reactivation following nitrogen starvation, which in addition to other factors driving mycobacterial recovery may also provide an opportunity for therapeutic intervention, especially in the serious infections caused by some emerging opportunistic pathogens of this group, such as Mycobacteroides abscessus (basonym Mycobacterium abscessus). Using a combination of biochemical methods and hybrid structural approaches, the oligomeric organization of M. hassiacum GgH was determined and molecular determinants of its substrate binding and specificity were unveiled.
		+
		+	The structural characterization of a glucosylglycerate hydrolase provides insights into the molecular mechanism of mycobacterial recovery from nitrogen starvation.,Cereija TB, Alarico S, Lourenco EC, Manso JA, Ventura MR, Empadinhas N, Macedo-Ribeiro S, Pereira PJB IUCrJ. 2019 May 8;6(Pt 4):572-585. doi: 10.1107/S2052252519005372. eCollection, 2019 Jul 1. PMID:31316802<ref>PMID:31316802</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ojv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:05, 21 May 2019

2019-05-21T08:05:32Z

←Older revision		Revision as of 08:05, 21 May 2019
Line 1:		Line 1:

-	==~~Mycobacterial~~ hydrolase complex ~~10.~~==	+	==Crystal structure of Mycolicibacterium hassiacum glucosylglycerate hydrolase (MhGgH) E419A variant in complex with mannosylglycerate==
-	<StructureSection load='5ojv' size='340' side='right' caption='[[5ojv]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='5ojv' size='340' side='right'caption='[[5ojv]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ojv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OJV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ojv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mychd Mychd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OJV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2M8:2-O-ALPHA-MANNOSYL-D-GLYCERATE'>2M8</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2M8:2-O-ALPHA-MANNOSYL-D-GLYCERATE'>2M8</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">C731_0006 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1122247 MYCHD])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ojv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojv OCA], [http://pdbe.org/5ojv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ojv RCSB], [http://www.ebi.ac.uk/pdbsum/5ojv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ojv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojv OCA], [http://pdbe.org/5ojv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ojv RCSB], [http://www.ebi.ac.uk/pdbsum/5ojv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojv ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mychd]]
	[[Category: Cereija, T B]]		[[Category: Cereija, T B]]
	[[Category: Macedo-Ribeiro, S]]		[[Category: Macedo-Ribeiro, S]]

OCA at 21:15, 9 August 2018

2018-08-09T21:15:45Z

←Older revision		Revision as of 21:15, 9 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ojv is ~~ON HOLD until Paper Publication~~	+	==Mycobacterial hydrolase complex 10.==
-		+	<StructureSection load='5ojv' size='340' side='right' caption='[[5ojv]], [[Resolution\|resolution]] 2.06Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ojv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OJV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OJV FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2M8:2-O-ALPHA-MANNOSYL-D-GLYCERATE'>2M8</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ojv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ojv OCA], [http://pdbe.org/5ojv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ojv RCSB], [http://www.ebi.ac.uk/pdbsum/5ojv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ojv ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cereija, T B]]
		+	[[Category: Macedo-Ribeiro, S]]
		+	[[Category: Pereira, P J.B]]
		+	[[Category: Hydrolase]]
		+	[[Category: Mycobacterium]]

OCA: Protected "5ojv" [edit=sysop:move=sysop]

2017-09-27T12:30:54Z

Protected "5ojv" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:30, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5ojv is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2017-09-27T12:30:53Z

New page: '''Unreleased structure''' The entry 5ojv is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5ojv is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]