5ole - Revision history

OCA at 16:53, 13 December 2023

2023-12-13T16:53:52Z

←Older revision		Revision as of 16:53, 13 December 2023
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	<StructureSection load='5ole' size='340' side='right'caption='[[5ole]], [[Resolution\|resolution]] 1.78Å' scene=''>		<StructureSection load='5ole' size='340' side='right'caption='[[5ole]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ole]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OLE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ole]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OLE FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.78Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ole FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ole OCA], [~~http~~://pdbe.org/5ole PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ole RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ole PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ole ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ole FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ole OCA], [https://pdbe.org/5ole PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ole RCSB], [https://www.ebi.ac.uk/pdbsum/5ole PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ole ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>	+	[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5ole" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5ole" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lysozyme 3D structures\|Lysozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Ferraro G]]
-	[[Category: Ferraro, G]]	+	[[Category: Merlino A]]
-	[[Category: Merlino, A]]	+
-	~~[[Category: Adduct]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Platinum]]~~	+
-	~~[[Category: Ribonuclease]]~~	+
-	~~[[Category: Tetraplatinum~~]]	+

OCA at 12:32, 13 March 2019

2019-03-13T12:32:32Z

←Older revision		Revision as of 12:32, 13 March 2019
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	==X-ray structure of the adduct formed upon reaction of hen egg white lysozyme with a tetranuclear Pt-thiosemicarbazone compound==		==X-ray structure of the adduct formed upon reaction of hen egg white lysozyme with a tetranuclear Pt-thiosemicarbazone compound==
-	<StructureSection load='5ole' size='340' side='right' caption='[[5ole]], [[Resolution\|resolution]] 1.78Å' scene=''>	+	<StructureSection load='5ole' size='340' side='right'caption='[[5ole]], [[Resolution\|resolution]] 1.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ole]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OLE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ole]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OLE FirstGlance]. <br>
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>		[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The tetranuclear Pt complex (PtL)4 (where L(2-) is the anion derived from para-isopropyl thiosemicarbazone) was first described in A.G. Quiroga et al., J. Med. Chem. 41, 1998, 1399-1408. (PtL)4 manifests antiproliferative properties toward various cancer cell lines being a promising anticancer drug candidate. Yet, details of its reactivity with biomolecules have not been elucidated. To this end, we investigated the reactions of (PtL)4 with a few model proteins, i.e. bovine pancreatic ribonuclease (RNase A), cytochrome c (Cyt c) and hen egg white lysozyme (Lysozyme), through electrospray ionization mass spectrometry and other biophysical methods. A rich reactivity of (PtL)4 with the above-mentioned model proteins is observed, leading to the formation of numerous metallodrug-protein adducts. The tetranuclear complex breaks down and various fragments bind proteins up to high metal/protein ratios; this typically results into very complicated mass spectral patterns. However, some of the main mass peaks could be assigned in the case of the Lysozyme adduct. In addition, crystallographic data were obtained for the (PtL)4/Lysozyme and (PtL)4/RNase A adducts pointing at His side chains as the primary binding sites for monometallic Pt fragments. Notably, a few selected features of the interactions observed in the (PtL)4/protein adducts were reproduced by reacting (PtL)4 with a small molecule, i.e. N-methylimidazole. In conclusion, the present study confirms the prodrug nature of the tetraplatinum complex, clarifies one possible pathway for its activation through cluster disassembly and allows initial identification of adducts formed with a representative protein.
		+
		+	Reactions of a tetranuclear Pt-thiosemicarbazone complex with model proteins.,Marzo T, Navas F, Cirri D, Merlino A, Ferraro G, Messori L, Quiroga AG J Inorg Biochem. 2018 Apr;181:11-17. doi: 10.1016/j.jinorgbio.2018.01.002. Epub, 2018 Jan 10. PMID:29353085<ref>PMID:29353085</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ole" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Gallus gallus]]		[[Category: Gallus gallus]]
		+	[[Category: Large Structures]]
	[[Category: Lysozyme]]		[[Category: Lysozyme]]
	[[Category: Ferraro, G]]		[[Category: Ferraro, G]]

OCA at 07:15, 29 August 2018

2018-08-29T07:15:48Z

←Older revision		Revision as of 07:15, 29 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5ole is ON HOLD until Paper Publication~~	+	==X-ray structure of the adduct formed upon reaction of hen egg white lysozyme with a tetranuclear Pt-thiosemicarbazone compound==
-		+	<StructureSection load='5ole' size='340' side='right' caption='[[5ole]], [[Resolution\|resolution]] 1.78Å' scene=''>
-	~~Authors: Ferraro, G., Merlino, A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ole]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OLE FirstGlance]. <br>
-	~~Description:~~ X-ray structure of the adduct formed upon reaction of hen egg white lysozyme with a tetranuclear Pt-thiosemicarbazone compound	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PT:PLATINUM+(II)+ION'>PT</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ole FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ole OCA], [http://pdbe.org/5ole PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ole RCSB], [http://www.ebi.ac.uk/pdbsum/5ole PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ole ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Lysozyme]]
	[[Category: Ferraro, G]]		[[Category: Ferraro, G]]
	[[Category: Merlino, A]]		[[Category: Merlino, A]]
		+	[[Category: Adduct]]
		+	[[Category: Hydrolase]]
		+	[[Category: Platinum]]
		+	[[Category: Ribonuclease]]
		+	[[Category: Tetraplatinum]]

OCA: Protected "5ole" [edit=sysop:move=sysop]

2017-09-27T12:33:30Z

Protected "5ole" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:33, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5ole is ON HOLD until Paper Publication Authors: Ferraro, G., Merlino, A. Description: X-ray structure of the adduct formed upon reaction of hen eg...

2017-09-27T12:33:28Z

New page: '''Unreleased structure''' The entry 5ole is ON HOLD until Paper Publication Authors: Ferraro, G., Merlino, A. Description: X-ray structure of the adduct formed upon reaction of hen eg...

New page

'''Unreleased structure'''

The entry 5ole is ON HOLD until Paper Publication

Authors: Ferraro, G., Merlino, A.

Description: X-ray structure of the adduct formed upon reaction of hen egg white lysozyme with a tetranuclear Pt-thiosemicarbazone compound
[[Category: Unreleased Structures]]
[[Category: Ferraro, G]]
[[Category: Merlino, A]]