5olx - Revision history

OCA at 16:54, 13 December 2023

2023-12-13T16:54:17Z

←Older revision		Revision as of 16:54, 13 December 2023
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	==5-fluorotryptophan labeled beta-phosphoglucomutase in a closed conformation, orthorhomic crystal form==		==5-fluorotryptophan labeled beta-phosphoglucomutase in a closed conformation, orthorhomic crystal form==
-	<StructureSection load='5olx' size='340' side='right' caption='[[5olx]], [[Resolution\|resolution]] 1.38Å' scene=''>	+	<StructureSection load='5olx' size='340' side='right'caption='[[5olx]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5olx]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"lactococcus_lactis_subsp~~.~~_diacitilactis_(sic)"_(garvie_and_farrow_1982)_schleifer_et_al._1986 "lactococcus~~ lactis subsp. ~~diacitilactis (sic)" (garvie and farrow 1982) schleifer et al. 1986~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5OLX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5olx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis Lactococcus lactis subsp. lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OLX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=BG6:BETA~~-~~D-GLUCOSE-6-PHOSPHATE'>BG6</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MGF:TRIFLUOROMAGNESATE'>MGF</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.38Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~FTR~~:~~FLUOROTRYPTOPHANE~~'>~~FTR~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BG6:BETA-D-GLUCOSE-6-PHOSPHATE'>BG6</scene>, <scene name='pdbligand=FTR:FLUOROTRYPTOPHANE'>FTR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MGF:TRIFLUOROMAGNESATE'>MGF</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">pgmB~~, ~~LL0429, L0001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1360 "Lactococcus lactis subsp. diacitilactis (sic)" (Garvie and Farrow 1982) Schleifer et al. 1986])~~<~~/td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5olx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5olx OCA], [https://pdbe.org/5olx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5olx RCSB], [https://www.ebi.ac.uk/pdbsum/5olx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5olx ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5olx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5olx OCA], [~~http~~://pdbe.org/5olx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5olx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5olx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5olx ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PGMB_LACLA PGMB_LACLA]] Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.<ref>PMID:9084169</ref> <ref>PMID:15005616</ref>	+	[https://www.uniprot.org/uniprot/PGMB_LACLA PGMB_LACLA] Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.<ref>PMID:9084169</ref> <ref>PMID:15005616</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 21:

	==See Also==		==See Also==
-	*[[Beta-phosphoglucomutase\|Beta-phosphoglucomutase]]	+	*[[Beta-phosphoglucomutase 3D structures\|Beta-phosphoglucomutase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Beta-phosphoglucomutase~~]]	+	[[Category: Lactococcus lactis subsp. lactis]]
-	[[Category: ~~Bowler, M W~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Velsen, J von~~]]	+	[[Category: Bowler MW]]
-	[[Category: ~~19f-nmr]]~~	+	[[Category: Von Velsen J]]
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Nmr labeling]]~~	+
-	~~[[Category: Phosphoryl transfer~~]]	+

OCA at 06:29, 18 April 2018

2018-04-18T06:29:11Z

←Older revision		Revision as of 06:29, 18 April 2018
Line 12:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/PGMB_LACLA PGMB_LACLA]] Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.<ref>PMID:9084169</ref> <ref>PMID:15005616</ref>		[[http://www.uniprot.org/uniprot/PGMB_LACLA PGMB_LACLA]] Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.<ref>PMID:9084169</ref> <ref>PMID:15005616</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ternary transition state analogue (TSA) complexes probing the isomerization of beta-d-glucose 1-phosphate (G1P) into d-glucose 6-phosphate (G6P) catalyzed by catalytically active, fluorinated (5-fluorotryptophan), beta-phosphoglucomutase (betaPGM) have been observed directly by (19)F NMR spectroscopy. In these complexes MgF3(-) and AlF4(-) are surrogates for the transferring phosphate. However, the relevance of these metal fluorides as TSA complexes has been queried. The 1D (19)F spectrum of a ternary TSA complex presented a molar equivalence between fluorinated enzyme, metal fluoride and non-isomerizable fluoromethylenephosphonate substrate analogue. Ring flips of the 5-fluoroindole ring remote from the active site were observed by both (19)F NMR and X-ray crystallography, but did not perturb function. This data unequivocally demonstrates that the concentration of the metal fluoride complexes is equivalent to the concentration of enzyme and ligand in the TSA complex in aqueous solution.
		+
		+	Observing enzyme ternary transition state analogue complexes by (19)F NMR spectroscopy.,Ampaw A, Carroll M, von Velsen J, Bhattasali D, Cohen A, Bowler MW, Jakeman DL Chem Sci. 2017 Dec 1;8(12):8427-8434. doi: 10.1039/c7sc04204c. Epub 2017 Oct 23. PMID:29619190<ref>PMID:29619190</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5olx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-phosphoglucomutase\|Beta-phosphoglucomutase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:13, 15 November 2017

2017-11-15T10:13:42Z

←Older revision		Revision as of 10:13, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5olx' size='340' side='right' caption='[[5olx]], [[Resolution\|resolution]] 1.38Å' scene=''>		<StructureSection load='5olx' size='340' side='right' caption='[[5olx]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5olx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OLX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5olx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"lactococcus_lactis_subsp._diacitilactis_(sic)"_(garvie_and_farrow_1982)_schleifer_et_al._1986 "lactococcus lactis subsp. diacitilactis (sic)" (garvie and farrow 1982) schleifer et al. 1986]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OLX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BG6:BETA-D-GLUCOSE-6-PHOSPHATE'>BG6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MGF:TRIFLUOROMAGNESATE'>MGF</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BG6:BETA-D-GLUCOSE-6-PHOSPHATE'>BG6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MGF:TRIFLUOROMAGNESATE'>MGF</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FTR:FLUOROTRYPTOPHANE'>FTR</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FTR:FLUOROTRYPTOPHANE'>FTR</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pgmB, LL0429, L0001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1360 "Lactococcus lactis subsp. diacitilactis (sic)" (Garvie and Farrow 1982) Schleifer et al. 1986])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5olx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5olx OCA], [http://pdbe.org/5olx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5olx RCSB], [http://www.ebi.ac.uk/pdbsum/5olx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5olx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5olx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5olx OCA], [http://pdbe.org/5olx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5olx RCSB], [http://www.ebi.ac.uk/pdbsum/5olx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5olx ProSAT]</span></td></tr>

OCA at 06:28, 1 November 2017

2017-11-01T06:28:08Z

←Older revision		Revision as of 06:28, 1 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5olx is ~~ON HOLD~~	+	==5-fluorotryptophan labeled beta-phosphoglucomutase in a closed conformation, orthorhomic crystal form==
-		+	<StructureSection load='5olx' size='340' side='right' caption='[[5olx]], [[Resolution\|resolution]] 1.38Å' scene=''>
-	~~Authors~~: ~~Bowler~~, M.W., ~~von Velsen~~, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5olx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OLX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OLX FirstGlance]. <br>
-	~~Description~~: 5-~~flurotryptophan labeled~~ beta-phosphoglucomutase in ~~an open conformation~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BG6:BETA-D-GLUCOSE-6-PHOSPHATE'>BG6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MGF:TRIFLUOROMAGNESATE'>MGF</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=FTR:FLUOROTRYPTOPHANE'>FTR</scene></td></tr>
-	[[Category: Bowler, M.W]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-phosphoglucomutase Beta-phosphoglucomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.2.6 5.4.2.6] </span></td></tr>
-	[[Category: ~~Von~~ Velsen, J]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5olx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5olx OCA], [http://pdbe.org/5olx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5olx RCSB], [http://www.ebi.ac.uk/pdbsum/5olx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5olx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PGMB_LACLA PGMB_LACLA]] Catalyzes the interconversion of D-glucose 1-phosphate (G1P) and D-glucose 6-phosphate (G6P), forming beta-D-glucose 1,6-(bis)phosphate (beta-G16P) as an intermediate. The beta-phosphoglucomutase (Beta-PGM) acts on the beta-C(1) anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose.<ref>PMID:9084169</ref> <ref>PMID:15005616</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Beta-phosphoglucomutase]]
		+	[[Category: Bowler, M W]]
		+	[[Category: Velsen, J von]]
		+	[[Category: 19f-nmr]]
		+	[[Category: Isomerase]]
		+	[[Category: Nmr labeling]]
		+	[[Category: Phosphoryl transfer]]

OCA: Protected "5olx" [edit=sysop:move=sysop]

2017-09-27T12:34:41Z

Protected "5olx" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:34, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5olx is ON HOLD Authors: Bowler, M.W., von Velsen, J. Description: 5-flurotryptophan labeled beta-phosphoglucomutase in an open conformation [[Cate...

2017-09-27T12:34:40Z

New page: '''Unreleased structure''' The entry 5olx is ON HOLD Authors: Bowler, M.W., von Velsen, J. Description: 5-flurotryptophan labeled beta-phosphoglucomutase in an open conformation [[Cate...

New page

'''Unreleased structure'''

The entry 5olx is ON HOLD

Authors: Bowler, M.W., von Velsen, J.

Description: 5-flurotryptophan labeled beta-phosphoglucomutase in an open conformation
[[Category: Unreleased Structures]]
[[Category: Bowler, M.W]]
[[Category: Von Velsen, J]]