5ptd - Revision history

OCA at 09:50, 22 May 2024

2024-05-22T09:50:46Z

←Older revision		Revision as of 09:50, 22 May 2024
Line 3:		Line 3:
	<StructureSection load='5ptd' size='340' side='right'caption='[[5ptd]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5ptd' size='340' side='right'caption='[[5ptd]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_14579 Atcc 14579~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5PTD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5PTD FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>PI-PLC GENE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=1396 ATCC 14579])</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~">~~<b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol~~-~~lyase Phosphatidylinositol diacylglycerol-lyase]~~, ~~with EC number~~ [~~https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [https://pdbe.org/5ptd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [https://www.ebi.ac.uk/pdbsum/5ptd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ptd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [https://pdbe.org/5ptd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [https://www.ebi.ac.uk/pdbsum/5ptd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ptd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PLC_BACCE PLC_BACCE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates.	+	[https://www.uniprot.org/uniprot/PLC_BACCE PLC_BACCE] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 14579~~]]	+	[[Category: Bacillus cereus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phosphatidylinositol diacylglycerol-lyase]]~~	+	[[Category: Heinz DW]]
-	[[Category: Heinz~~, D W]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Lipid degradation]]~~	+
-	~~[[Category: Phosphatidylinositol specific phospholipase c]]~~	+
-	~~[[Category: Phosphoric diester~~]]	+

OCA at 15:51, 3 November 2021

2021-11-03T15:51:38Z

←Older revision		Revision as of 15:51, 3 November 2021
Line 1:		Line 1:
		+
	==PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT H32A==		==PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT H32A==
-	<StructureSection load='5ptd' size='340' side='right' caption='[[5ptd]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='5ptd' size='340' side='right'caption='[[5ptd]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5PTD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5PTD FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PI-PLC GENE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 ATCC 14579])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PI-PLC GENE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 ATCC 14579])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [~~http~~://pdbe.org/5ptd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ptd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [https://pdbe.org/5ptd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [https://www.ebi.ac.uk/pdbsum/5ptd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ptd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PLC_BACCE PLC_BACCE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates.	+	[[https://www.uniprot.org/uniprot/PLC_BACCE PLC_BACCE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/5ptd_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/5ptd_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 31:

	==See Also==		==See Also==
		+	*[[Bromodomain-containing protein 3D structures\|Bromodomain-containing protein 3D structures]]
	*[[Phospholipase C\|Phospholipase C]]		*[[Phospholipase C\|Phospholipase C]]
	== References ==		== References ==
Line 36:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 14579]]		[[Category: Atcc 14579]]
		+	[[Category: Large Structures]]
	[[Category: Phosphatidylinositol diacylglycerol-lyase]]		[[Category: Phosphatidylinositol diacylglycerol-lyase]]
	[[Category: Heinz, D W]]		[[Category: Heinz, D W]]

OCA at 05:20, 9 February 2016

2016-02-09T05:20:12Z

←Older revision		Revision as of 05:20, 9 February 2016
Line 2:		Line 2:
	<StructureSection load='5ptd' size='340' side='right' caption='[[5ptd]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='5ptd' size='340' side='right' caption='[[5ptd]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_cereus Bacillus cereus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5PTD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5PTD FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PI-PLC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 ~~Bacillus cereus~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PI-PLC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 ATCC 14579])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [http://www.ebi.ac.uk/pdbsum/5ptd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [http://pdbe.org/5ptd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [http://www.ebi.ac.uk/pdbsum/5ptd PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5ptd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 5ptd" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[~~Phosphatidylinositol-specific phospholipase~~ C\|~~Phosphatidylinositol-specific phospholipase~~ C]]	+	*[[Phospholipase C\|Phospholipase C]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus cereus~~]]	+	[[Category: Atcc 14579]]
	[[Category: Phosphatidylinositol diacylglycerol-lyase]]		[[Category: Phosphatidylinositol diacylglycerol-lyase]]
	[[Category: Heinz, D W]]		[[Category: Heinz, D W]]

OCA at 04:42, 25 December 2014

2014-12-25T04:42:33Z

←Older revision		Revision as of 04:42, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5PTD FirstGlance]. <br>		<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5PTD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PI-PLC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 Bacillus cereus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PI-PLC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 Bacillus cereus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [http://www.ebi.ac.uk/pdbsum/5ptd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [http://www.ebi.ac.uk/pdbsum/5ptd PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PLC_BACCE PLC_BACCE]] Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 25:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+
		+	==See Also==
		+	*[[Phosphatidylinositol-specific phospholipase C\|Phosphatidylinositol-specific phospholipase C]]
	== References ==		== References ==
	<references/>		<references/>
Line 31:		Line 36:
	[[Category: Bacillus cereus]]		[[Category: Bacillus cereus]]
	[[Category: Phosphatidylinositol diacylglycerol-lyase]]		[[Category: Phosphatidylinositol diacylglycerol-lyase]]
-	[[Category: Heinz, D W.]]	+	[[Category: Heinz, D W]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Lipid degradation]]		[[Category: Lipid degradation]]
	[[Category: Phosphatidylinositol specific phospholipase c]]		[[Category: Phosphatidylinositol specific phospholipase c]]
	[[Category: Phosphoric diester]]		[[Category: Phosphoric diester]]

OCA: Protected "5ptd" [edit=sysop:move=sysop]

2014-06-05T08:35:12Z

Protected "5ptd" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:35, 5 June 2014

OCA at 08:35, 5 June 2014

2014-06-05T08:35:10Z

←Older revision		Revision as of 08:35, 5 June 2014
Line 1:		Line 1:
-	~~{{Seed}}~~	+	==PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT H32A==
-	[[~~Image~~:5ptd.~~png\|left~~\|200px]]	+	<StructureSection load='5ptd' size='340' side='right' caption='[[5ptd]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ptd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5PTD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PI-PLC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 Bacillus cereus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB], [http://www.ebi.ac.uk/pdbsum/5ptd PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pt/5ptd_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The role of amino acid residues located in the active site pocket of phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus[Heinz, D. W., Ryan, M., Bullock, T., & Griffith, O. H. (1995) EMBO J. 14, 3855-3863] was investigated by site-directed mutagenesis, kinetics, and crystal structure analysis. Twelve residues involved in catalysis and substrate binding (His32, Arg69, His82, Gly83, Lys115, Glu117, Arg163, Trp178, Asp180, Asp198, Tyr200, and Asp274) were individually replaced by 1-3 other amino acids, resulting in a total number of 21 mutants. Replacements in the mutants H32A, H32L, R69A, R69E, R69K, H82A, H82L, E117K, R163I, D198A, D198E, D198S, Y200S, and D274S caused essentially complete inactivation of the enzyme. The remaining mutants (G83S, K115E, R163K, W178Y, D180S, Y200F, and D274N) exhibited reduced activities up to 57% when compared with wild-type PI-PLC. Crystal structures determined at a resolution ranging from 2.0 to 2.7 A for six mutants (H32A, H32L, R163K, D198E, D274N, and D274S) showed that significant changes were confined to the site of the respective mutation without perturbation of the rest of the structure. Only in mutant D198E do the side chains of two neighboring arginine residues move across the inositol binding pocket toward the newly introduced glutamic acid. An analysis of these structure-function relationships provides new insight into the catalytic mechanism, and suggests a molecular explanation of some of the substrate stereospecificity and inhibitor binding data available for this enzyme.

-	<!--	+	Probing the roles of active site residues in phosphatidylinositol-specific phospholipase C from Bacillus cereus by site-directed mutagenesis.,Gassler CS, Ryan M, Liu T, Griffith OH, Heinz DW Biochemistry. 1997 Oct 21;36(42):12802-13. PMID:9335537<ref>PMID:9335537</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_5ptd"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_5ptd\| PDB=5ptd \| SCENE= }}~~	+

-	~~===PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT H32A===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_9335537}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 9335537 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_9335537}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~5PTD is a 1 chain structure of sequence from [http:~~/~~/en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:9335537<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus cereus]]		[[Category: Bacillus cereus]]
	[[Category: Phosphatidylinositol diacylglycerol-lyase]]		[[Category: Phosphatidylinositol diacylglycerol-lyase]]
Line 31:		Line 36:
	[[Category: Phosphatidylinositol specific phospholipase c]]		[[Category: Phosphatidylinositol specific phospholipase c]]
	[[Category: Phosphoric diester]]		[[Category: Phosphoric diester]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:47:18 2009''

OCA at 04:47, 17 February 2009

2009-02-17T04:47:20Z

←Older revision		Revision as of 04:47, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	5PTD is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA].	+	5PTD is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5PTD OCA].

	==Reference==		==Reference==
-	Probing the roles of active site residues in phosphatidylinositol-specific phospholipase C from Bacillus cereus by site-directed mutagenesis., Gassler CS, Ryan M, Liu T, Griffith OH, Heinz DW, Biochemistry. 1997 Oct 21;36(42):12802-13. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9335537 9335537]~~	+	<ref group="xtra">PMID:9335537</ref><references group="xtra"/>
	[[Category: Bacillus cereus]]		[[Category: Bacillus cereus]]
	[[Category: Phosphatidylinositol diacylglycerol-lyase]]		[[Category: Phosphatidylinositol diacylglycerol-lyase]]
-	[[Category: Single protein]]
	[[Category: Heinz, D W.]]		[[Category: Heinz, D W.]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
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	[[Category: Phosphoric diester]]		[[Category: Phosphoric diester]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 14~~:09:~~22 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:47:18 2009''

OCA at 11:09, 3 July 2008

2008-07-03T11:09:23Z

←Older revision		Revision as of 11:09, 3 July 2008
Line 1:		Line 1:
-	[[Image:5ptd.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:5ptd.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_5ptd\| PDB=5ptd \| SCENE= }}		{{STRUCTURE_5ptd\| PDB=5ptd \| SCENE= }}

-	~~'''~~PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT H32A~~'''~~	+	===PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT H32A===


-	~~==Overview==~~	+	<!--
-	The ~~role of amino acid residues located in the active site pocket of phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus[Heinz~~, ~~D. W.~~, Ryan, M., Bullock, T., & Griffith, O. H. (1995) EMBO J. 14, 3855-3863] was investigated by site-directed mutagenesis, kinetics, and crystal structure analysis. Twelve residues involved in catalysis and substrate binding (His32, Arg69, His82, Gly83, Lys115, Glu117, Arg163, Trp178, Asp180, Asp198, Tyr200, and Asp274) were individually replaced by 1-3 other amino acids, resulting in a total number of 21 mutants. Replacements in the ~~mutants H32A, H32L, R69A, R69E, R69K, H82A, H82L, E117K, R163I, D198A, D198E, D198S, Y200S, and D274S caused essentially complete inactivation of~~ the ~~enzyme. The remaining mutants~~ (~~G83S, K115E, R163K, W178Y, D180S, Y200F, and D274N) exhibited reduced activities up to 57% when compared with wild-type PI-PLC. Crystal structures determined~~ at ~~a resolution ranging from 2~~.~~0 to 2~~.~~7 A for six mutants (H32A~~, ~~H32L, R163K, D198E, D274N, and D274S) showed that significant changes were confined to~~ the ~~site of the respective mutation without perturbation of the rest of the structure~~. ~~Only in mutant D198E do the side chains of two neighboring arginine residues move across the inositol binding pocket toward the newly introduced glutamic acid. An analysis of these structure~~-function relationships provides new insight into the catalytic mechanism, and suggests a molecular explanation of some of the substrate stereospecificity and inhibitor binding data available for this enzyme.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9335537}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9335537 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9335537}}

	==About this Structure==		==About this Structure==
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	[[Category: Phosphatidylinositol specific phospholipase c]]		[[Category: Phosphatidylinositol specific phospholipase c]]
	[[Category: Phosphoric diester]]		[[Category: Phosphoric diester]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:37:08 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 14:09:22 2008''

OCA at 19:37, 4 May 2008

2008-05-04T19:37:09Z

←Older revision		Revision as of 19:37, 4 May 2008
Line 1:		Line 1:
	[[Image:5ptd.gif\|left\|200px]]		[[Image:5ptd.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 5ptd \|SIZE=350\|CAPTION=~~ <~~scene name='initialview01'>5ptd</scene>, resolution 2.7Å~~	+	The line below this paragraph, containing "STRUCTURE_5ptd", creates the "Structure Box" on the page.
-	~~\|SITE= <scene name='pdbsite=ACT:Active+Site'>ACT</scene>~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND=~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol~~-~~lyase Phosphatidylinositol diacylglycerol~~-~~lyase]~~, ~~with EC number [http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span>~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= PI-PLC GENE~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 Bacillus cereus]~~)	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_5ptd\| PDB=5ptd \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	~~\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca~~-~~docs/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca~~-~~bin/ocaids?id~~=5ptd ~~OCA], [http://www.ebi.ac.uk/pdbsum/5ptd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId~~=~~5ptd RCSB]</span>~~	+
-	}}	+

	'''PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT H32A'''		'''PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C MUTANT H32A'''
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	[[Category: Single protein]]		[[Category: Single protein]]
	[[Category: Heinz, D W.]]		[[Category: Heinz, D W.]]
-	[[Category: ~~hydrolase~~]]	+	[[Category: Hydrolase]]
-	[[Category: ~~lipid~~ degradation]]	+	[[Category: Lipid degradation]]
-	[[Category: ~~phosphatidylinositol~~ specific phospholipase c]]	+	[[Category: Phosphatidylinositol specific phospholipase c]]
-	[[Category: ~~phosphoric~~ diester]]	+	[[Category: Phosphoric diester]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:37:08 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:42:06 2008''	+

OCA at 02:42, 31 March 2008

2008-03-31T02:42:08Z

←Older revision		Revision as of 02:42, 31 March 2008
Line 5:		Line 5:
	\|SITE= <scene name='pdbsite=ACT:Active+Site'>ACT</scene>		\|SITE= <scene name='pdbsite=ACT:Active+Site'>ACT</scene>
	\|LIGAND=		\|LIGAND=
-	\|ACTIVITY= [http://en.wikipedia.org/wiki/~~Transferred_entry:_4.6.1.13 Transferred entry: 4.6.1.13~~], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.~~10 3~~.1.~~4.10~~]	+	\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphatidylinositol_diacylglycerol-lyase Phosphatidylinositol diacylglycerol-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.13 4.6.1.13] </span>
	\|GENE= PI-PLC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 Bacillus cereus])		\|GENE= PI-PLC GENE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1396 Bacillus cereus])
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ptd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ptd OCA], [http://www.ebi.ac.uk/pdbsum/5ptd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=5ptd RCSB]</span>
	}}		}}

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	Probing the roles of active site residues in phosphatidylinositol-specific phospholipase C from Bacillus cereus by site-directed mutagenesis., Gassler CS, Ryan M, Liu T, Griffith OH, Heinz DW, Biochemistry. 1997 Oct 21;36(42):12802-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9335537 9335537]		Probing the roles of active site residues in phosphatidylinositol-specific phospholipase C from Bacillus cereus by site-directed mutagenesis., Gassler CS, Ryan M, Liu T, Griffith OH, Heinz DW, Biochemistry. 1997 Oct 21;36(42):12802-13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9335537 9335537]
	[[Category: Bacillus cereus]]		[[Category: Bacillus cereus]]
		+	[[Category: Phosphatidylinositol diacylglycerol-lyase]]
	[[Category: Single protein]]		[[Category: Single protein]]
-	[[Category: Transferred entry: 4 6.1 13]]
	[[Category: Heinz, D W.]]		[[Category: Heinz, D W.]]
	[[Category: hydrolase]]		[[Category: hydrolase]]
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	[[Category: phosphoric diester]]		[[Category: phosphoric diester]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 19~~:12:41 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:42:06 2008''