5sul - Revision history

OCA at 12:48, 4 October 2023

2023-10-04T12:48:04Z

←Older revision		Revision as of 12:48, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5sul' size='340' side='right'caption='[[5sul]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='5sul' size='340' side='right'caption='[[5sul]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5sul]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5SUL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5sul]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5SUL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=U5P:URIDINE~~-~~5-MONOPHOSPHATE'>U5P</scene>~~, ~~<scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>GSY2, YLR258W, L8479.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~559292 Baker's yeast])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Glycogen(starch)_synthase Glycogen(starch) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.11 2.4.1.11]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5sul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5sul OCA], [https://pdbe.org/5sul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5sul RCSB], [https://www.ebi.ac.uk/pdbsum/5sul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5sul ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5sul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5sul OCA], [~~http~~://pdbe.org/5sul PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5sul RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5sul PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5sul ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GYS2_YEAST GYS2_YEAST]] Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.	+	[https://www.uniprot.org/uniprot/GYS2_YEAST GYS2_YEAST] Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hurley, T D~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Mahalingan, K K~~]]	+	[[Category: Hurley TD]]
-	[[Category: ~~Glycogen synthase inhibited state]]~~	+	[[Category: Mahalingan KK]]
-	~~[[Category: Phosphorylation]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 08:57, 18 December 2019

2019-12-18T08:57:10Z

←Older revision		Revision as of 08:57, 18 December 2019
Line 1:		Line 1:

	==Inhibited state structure of yGsy2p==		==Inhibited state structure of yGsy2p==
-	<StructureSection load='5sul' size='340' side='right' caption='[[5sul]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='5sul' size='340' side='right'caption='[[5sul]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5sul]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SUL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5sul]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SUL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GSY2, YLR258W, L8479.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycogen(starch)_synthase Glycogen(starch) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.11 2.4.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycogen(starch)_synthase Glycogen(starch) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.11 2.4.1.11] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5sul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5sul OCA], [http://pdbe.org/5sul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5sul RCSB], [http://www.ebi.ac.uk/pdbsum/5sul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5sul ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5sul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5sul OCA], [http://pdbe.org/5sul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5sul RCSB], [http://www.ebi.ac.uk/pdbsum/5sul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5sul ProSAT]</span></td></tr>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Hurley, T D]]		[[Category: Hurley, T D]]
	[[Category: Mahalingan, K K]]		[[Category: Mahalingan, K K]]

OCA at 03:46, 6 September 2017

2017-09-06T03:46:46Z

←Older revision		Revision as of 03:46, 6 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5sul is ~~ON HOLD until Paper~~ Publication	+	==Inhibited state structure of yGsy2p==
		+	<StructureSection load='5sul' size='340' side='right' caption='[[5sul]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5sul]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5SUL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5SUL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycogen(starch)_synthase Glycogen(starch) synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.11 2.4.1.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5sul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5sul OCA], [http://pdbe.org/5sul PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5sul RCSB], [http://www.ebi.ac.uk/pdbsum/5sul PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5sul ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GYS2_YEAST GYS2_YEAST]] Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glycogen synthase (GS) is the rate limiting enzyme in the synthesis of glycogen. Eukaryotic GS is negatively regulated by covalent phosphorylation and allosterically activated by glucose-6-phosphate (G-6-P). To gain structural insights into the inhibited state of the enzyme, we solved the crystal structure of yGsy2-R589A/R592A to a resolution of 3.3 A. The double mutant has an activity ratio similar to the phosphorylated enzyme and also retains the ability to be activated by G-6-P. When compared to the 2.88 A structure of the wild-type G-6-P activated enzyme, the crystal structure of the low-activity mutant showed that the N-terminal domain of the inhibited state is tightly held against the dimer-related interface thereby hindering acceptor access to the catalytic cleft. On the basis of these two structural observations, we developed a reversible redox regulatory feature in yeast GS by substituting cysteine residues for two highly conserved arginine residues. When oxidized, the cysteine mutant enzyme exhibits activity levels similar to the phosphorylated enzyme but cannot be activated by G-6-P. Upon reduction, the cysteine mutant enzyme regains normal activity levels and regulatory response to G-6-P activation.

-	~~Authors:~~ Mahalingan, ~~K.K.~~, ~~Hurley~~, T.D.	+	Redox Switch for the Inhibited State of Yeast Glycogen Synthase Mimics Regulation by Phosphorylation.,Mahalingan KK, Baskaran S, DePaoli-Roach AA, Roach PJ, Hurley TD Biochemistry. 2017 Jan 10;56(1):179-188. doi: 10.1021/acs.biochem.6b00884. Epub, 2016 Dec 20. PMID:27935293<ref>PMID:27935293</ref>

-	~~Description: Inhibited state structure~~ of ~~yGsy2p~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Hurley, T.D]]	+	<div class="pdbe-citations 5sul" style="background-color:#fffaf0;"></div>
-	[[Category: Mahalingan, K.K]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hurley, T D]]
		+	[[Category: Mahalingan, K K]]
		+	[[Category: Glycogen synthase inhibited state]]
		+	[[Category: Phosphorylation]]
		+	[[Category: Transferase]]

OCA at 13:16, 9 March 2017

2017-03-09T13:16:21Z

←Older revision		Revision as of 13:16, 9 March 2017
Line 7:		Line 7:
	Description: Inhibited state structure of yGsy2p		Description: Inhibited state structure of yGsy2p
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Mahalingan, K.K]]
	[[Category: Hurley, T.D]]		[[Category: Hurley, T.D]]
		+	[[Category: Mahalingan, K.K]]

OCA at 17:02, 9 December 2016

2016-12-09T17:02:47Z

←Older revision		Revision as of 17:02, 9 December 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5sul is ON HOLD	+	The entry 5sul is ON HOLD until Paper Publication

	Authors: Mahalingan, K.K., Hurley, T.D.		Authors: Mahalingan, K.K., Hurley, T.D.

OCA: Protected "5sul" [edit=sysop:move=sysop]

2016-08-10T14:42:36Z

Protected "5sul" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:42, 10 August 2016

OCA: New page: '''Unreleased structure''' The entry 5sul is ON HOLD Authors: Mahalingan, K.K., Hurley, T.D. Description: Inhibited state structure of yGsy2p Category: Unreleased Structures [[Cate...

2016-08-10T14:42:35Z

New page: '''Unreleased structure''' The entry 5sul is ON HOLD Authors: Mahalingan, K.K., Hurley, T.D. Description: Inhibited state structure of yGsy2p Category: Unreleased Structures [[Cate...

New page

'''Unreleased structure'''

The entry 5sul is ON HOLD

Authors: Mahalingan, K.K., Hurley, T.D.

Description: Inhibited state structure of yGsy2p
[[Category: Unreleased Structures]]
[[Category: Mahalingan, K.K]]
[[Category: Hurley, T.D]]