5t59 - Revision history

OCA at 14:19, 6 March 2024

2024-03-06T14:19:20Z

←Older revision		Revision as of 14:19, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='5t59' size='340' side='right'caption='[[5t59]], [[Resolution\|resolution]] 2.41Å' scene=''>		<StructureSection load='5t59' size='340' side='right'caption='[[5t59]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5t59]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T59 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5T59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5t59]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T59 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NHE:2~~-[~~N-CYCLOHEXYLAMINO~~]~~ETHANE+SULFONIC+ACID'>NHE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.405Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">[~~[5t51\|5t51]~~]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [~~http~~://pdbe.org/5t59 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [https://pdbe.org/5t59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [https://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q6CLK3_KLULA Q6CLK3_KLULA]
-	Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with the principal microtubule-binding component, the Ndc80 complex (Ndc80C). We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. MIND resembles an elongated, asymmetric Y; two globular heads project from a coiled-coil shaft. An N-terminal extension of Dsn1 from one head regulates interactions of the other head, blocking binding of Mif2 and COMA. Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C-terminal extension of Dsn1 recruits Ndc80C to the opposite end of the shaft. The structure and properties of MIND show how it integrates phospho-regulatory inputs for kinetochore assembly and disassembly.	+
-		+
-	~~Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.,Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC Cell. 2016 Nov 3;167(4)~~:~~1014-1027.e12. doi: 10.1016~~/~~j.cell.2016.10.011. Epub 2016, Oct 27. PMID:27881300<ref>PMID:27881300<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5t59" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Kluyveromyces lactis NRRL Y-1140]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dimitrova, Y]]	+	[[Category: Dimitrova Y]]
-	[[Category: Harrison~~, S C~~]]	+	[[Category: Harrison SC]]
-	[[Category: Jenni, S]]	+	[[Category: Jenni S]]
-	[[Category: Khin, Y]]	+	[[Category: Khin Y]]
-	[[Category: Valverde, R]]	+	[[Category: Valverde R]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Chromosome]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Kinetochore]]~~	+
-	~~[[Category: Mind]]~~	+
-	~~[[Category: Mis12]]~~	+
-	~~[[Category: Segregation~~]]	+

OCA at 11:22, 16 September 2020

2020-09-16T11:22:07Z

←Older revision		Revision as of 11:22, 16 September 2020
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	==Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly==		==Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly==
-	<StructureSection load='5t59' size='340' side='right' caption='[[5t59]], [[Resolution\|resolution]] 2.41Å' scene=''>	+	<StructureSection load='5t59' size='340' side='right'caption='[[5t59]], [[Resolution\|resolution]] 2.41Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5t59]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T59 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5T59 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5t59]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T59 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5T59 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5t51\|5t51]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5t51\|5t51]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [http://pdbe.org/5t59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [http://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [http://pdbe.org/5t59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [http://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Dimitrova, Y]]		[[Category: Dimitrova, Y]]
	[[Category: Harrison, S C]]		[[Category: Harrison, S C]]

OCA at 14:29, 22 December 2016

2016-12-22T14:29:51Z

←Older revision		Revision as of 14:29, 22 December 2016
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [http://pdbe.org/5t59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [http://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [http://pdbe.org/5t59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [http://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with the principal microtubule-binding component, the Ndc80 complex (Ndc80C). We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. MIND resembles an elongated, asymmetric Y; two globular heads project from a coiled-coil shaft. An N-terminal extension of Dsn1 from one head regulates interactions of the other head, blocking binding of Mif2 and COMA. Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C-terminal extension of Dsn1 recruits Ndc80C to the opposite end of the shaft. The structure and properties of MIND show how it integrates phospho-regulatory inputs for kinetochore assembly and disassembly.
		+
		+	Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.,Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC Cell. 2016 Nov 3;167(4):1014-1027.e12. doi: 10.1016/j.cell.2016.10.011. Epub 2016, Oct 27. PMID:27881300<ref>PMID:27881300</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5t59" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 19:58, 9 December 2016

2016-12-09T19:58:32Z

←Older revision		Revision as of 19:58, 9 December 2016
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 5t59 is ON HOLD until Paper Publication~~	+	==Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly==
-		+	<StructureSection load='5t59' size='340' side='right' caption='[[5t59]], [[Resolution\|resolution]] 2.41Å' scene=''>
-	~~Authors: Dimitrova, Y., Jenni, S., Valverde, R., Khin, Y., Harrison, S.C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5t59]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T59 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T59 FirstGlance]. <br>
-	~~Description:~~ Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5t51\|5t51]]</td></tr>
-	[[~~Category~~: ~~Khin, Y~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t59 OCA], [http://pdbe.org/5t59 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t59 RCSB], [http://www.ebi.ac.uk/pdbsum/5t59 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t59 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Harrison~~, S.C]]	+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Dimitrova, Y]]		[[Category: Dimitrova, Y]]
		+	[[Category: Harrison, S C]]
	[[Category: Jenni, S]]		[[Category: Jenni, S]]
		+	[[Category: Khin, Y]]
	[[Category: Valverde, R]]		[[Category: Valverde, R]]
		+	[[Category: Cell cycle]]
		+	[[Category: Chromosome]]
		+	[[Category: Complex]]
		+	[[Category: Kinetochore]]
		+	[[Category: Mind]]
		+	[[Category: Mis12]]
		+	[[Category: Segregation]]

OCA: Protected "5t59" [edit=sysop:move=sysop]

2016-09-09T15:43:42Z

Protected "5t59" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:43, 9 September 2016

OCA: New page: '''Unreleased structure''' The entry 5t59 is ON HOLD until Paper Publication Authors: Dimitrova, Y., Jenni, S., Valverde, R., Khin, Y., Harrison, S.C. Description: Structure of the MIN...

2016-09-09T15:43:40Z

New page: '''Unreleased structure''' The entry 5t59 is ON HOLD until Paper Publication Authors: Dimitrova, Y., Jenni, S., Valverde, R., Khin, Y., Harrison, S.C. Description: Structure of the MIN...

New page

'''Unreleased structure'''

The entry 5t59 is ON HOLD until Paper Publication

Authors: Dimitrova, Y., Jenni, S., Valverde, R., Khin, Y., Harrison, S.C.

Description: Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly
[[Category: Unreleased Structures]]
[[Category: Khin, Y]]
[[Category: Harrison, S.C]]
[[Category: Dimitrova, Y]]
[[Category: Jenni, S]]
[[Category: Valverde, R]]