5tgl - Revision history

OCA at 14:20, 6 March 2024

2024-03-06T14:20:38Z

←Older revision		Revision as of 14:20, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='5tgl' size='340' side='right'caption='[[5tgl]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5tgl' size='340' side='right'caption='[[5tgl]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tgl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_16457 Atcc 16457~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5TGL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tgl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TGL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEE:N~~-~~HEXYLPHOSPHONATE+ETHYL+ESTER'>HEE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEE:N-HEXYLPHOSPHONATE+ETHYL+ESTER'>HEE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5tgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgl OCA], [~~http~~://pdbe.org/5tgl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5tgl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5tgl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5tgl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgl OCA], [https://pdbe.org/5tgl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tgl RCSB], [https://www.ebi.ac.uk/pdbsum/5tgl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tgl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LIP_RHIMI LIP_RHIMI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5tgl ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5tgl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols. They have been classified as serine hydrolases owing to their inhibition by diethyl p-nitrophenyl phosphate. Lipase activity is greatly increased at the lipid-water interface, a phenomenon known as interfacial activation. X-ray analysis has revealed the atomic structures of two triacylglycerol lipases, unrelated in sequence: the human pancreatic lipase (hPL)4, and an enzyme isolated from the fungus Rhizomucor (formerly Mucor) miehei (RmL). In both enzymes the active centres contain structurally analogous Asp-His-Ser triads (characteristic of serine proteinases), which are buried completely beneath a short helical segment, or 'lid'. Here we present the crystal structure (at 3 A resolution) of a complex of R. miehei lipase with n-hexylphosphonate ethyl ester in which the enzyme's active site is exposed by the movement of the helical lid. This movement also increases the nonpolarity of the surface surrounding the catalytic site. We propose that the structure of the enzyme in this complex is equivalent to the activated state generated by the oil-water interface.
-
-	A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex.,Brzozowski AM, Derewenda U, Derewenda ZS, Dodson GG, Lawson DM, Turkenburg JP, Bjorkling F, Huge-Jensen B, Patkar SA, Thim L Nature. 1991 Jun 6;351(6326):491-4. PMID:2046751<ref>PMID:2046751</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 5tgl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Lipase 3D Structures\|Lipase 3D Structures]]		*[[Lipase 3D Structures\|Lipase 3D Structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 16457]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Triacylglycerol lipase~~]]	+	[[Category: Rhizomucor miehei]]
-	[[Category: Bjorkling, F]]	+	[[Category: Bjorkling F]]
-	[[Category: Brzozowski~~, A M~~]]	+	[[Category: Brzozowski AM]]
-	[[Category: Derewenda, U]]	+	[[Category: Derewenda U]]
-	[[Category: Derewenda~~, Z S~~]]	+	[[Category: Derewenda ZS]]
-	[[Category: Dodson~~, G G~~]]	+	[[Category: Dodson GG]]
-	[[Category: Huge-Jensen, B]]	+	[[Category: Huge-Jensen B]]
-	[[Category: Lawson, D]]	+	[[Category: Lawson D]]
-	[[Category: Patkar~~, S R~~]]	+	[[Category: Patkar SR]]
-	[[Category: Thim, L]]	+	[[Category: Thim L]]
-	[[Category: Turkenburg~~, J P~~]]	+	[[Category: Turkenburg JP]]

OCA at 12:10, 23 September 2020

2020-09-23T12:10:01Z

←Older revision		Revision as of 12:10, 23 September 2020
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		+
	==A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX==		==A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX==
-	<StructureSection load='5tgl' size='340' side='right' caption='[[5tgl]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5tgl' size='340' side='right'caption='[[5tgl]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tgl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16457 Atcc 16457]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5TGL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tgl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16457 Atcc 16457]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TGL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEE:N-HEXYLPHOSPHONATE+ETHYL+ESTER'>HEE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEE:N-HEXYLPHOSPHONATE+ETHYL+ESTER'>HEE</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5tgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgl OCA], [http://pdbe.org/5tgl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tgl RCSB], [http://www.ebi.ac.uk/pdbsum/5tgl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5tgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgl OCA], [http://pdbe.org/5tgl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tgl RCSB], [http://www.ebi.ac.uk/pdbsum/5tgl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tgl ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 11:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/5tgl_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/5tgl_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 28:		Line 29:

	==See Also==		==See Also==
-	*[[Lipase\|Lipase]]	+	*[[Lipase 3D Structures\|Lipase 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 16457]]		[[Category: Atcc 16457]]
		+	[[Category: Large Structures]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Bjorkling, F]]		[[Category: Bjorkling, F]]

OCA at 06:03, 7 February 2016

2016-02-07T06:03:03Z

←Older revision		Revision as of 06:03, 7 February 2016
Line 2:		Line 2:
	<StructureSection load='5tgl' size='340' side='right' caption='[[5tgl]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='5tgl' size='340' side='right' caption='[[5tgl]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tgl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhizomucor_miehei Rhizomucor miehei~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TGL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tgl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_16457 Atcc 16457]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TGL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEE:N-HEXYLPHOSPHONATE+ETHYL+ESTER'>HEE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEE:N-HEXYLPHOSPHONATE+ETHYL+ESTER'>HEE</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5tgl RCSB], [http://www.ebi.ac.uk/pdbsum/5tgl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgl OCA], [http://pdbe.org/5tgl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tgl RCSB], [http://www.ebi.ac.uk/pdbsum/5tgl PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5tgl ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 5tgl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rhizomucor miehei~~]]	+	[[Category: Atcc 16457]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Bjorkling, F]]		[[Category: Bjorkling, F]]

OCA at 16:47, 10 December 2014

2014-12-10T16:47:27Z

←Older revision		Revision as of 16:47, 10 December 2014
Line 1:		Line 1:
-	[[~~Image~~:5tgl.~~png\|left~~\|200px]]	+	==A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX==
		+	<StructureSection load='5tgl' size='340' side='right' caption='[[5tgl]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5tgl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TGL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEE:N-HEXYLPHOSPHONATE+ETHYL+ESTER'>HEE</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tgl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tgl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5tgl RCSB], [http://www.ebi.ac.uk/pdbsum/5tgl PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tg/5tgl_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols. They have been classified as serine hydrolases owing to their inhibition by diethyl p-nitrophenyl phosphate. Lipase activity is greatly increased at the lipid-water interface, a phenomenon known as interfacial activation. X-ray analysis has revealed the atomic structures of two triacylglycerol lipases, unrelated in sequence: the human pancreatic lipase (hPL)4, and an enzyme isolated from the fungus Rhizomucor (formerly Mucor) miehei (RmL). In both enzymes the active centres contain structurally analogous Asp-His-Ser triads (characteristic of serine proteinases), which are buried completely beneath a short helical segment, or 'lid'. Here we present the crystal structure (at 3 A resolution) of a complex of R. miehei lipase with n-hexylphosphonate ethyl ester in which the enzyme's active site is exposed by the movement of the helical lid. This movement also increases the nonpolarity of the surface surrounding the catalytic site. We propose that the structure of the enzyme in this complex is equivalent to the activated state generated by the oil-water interface.

-	~~{{STRUCTURE_5tgl\| PDB=5tgl \| SCENE= }}~~	+	A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex.,Brzozowski AM, Derewenda U, Derewenda ZS, Dodson GG, Lawson DM, Turkenburg JP, Bjorkling F, Huge-Jensen B, Patkar SA, Thim L Nature. 1991 Jun 6;351(6326):491-4. PMID:2046751<ref>PMID:2046751</ref>

-	~~===A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_2046751}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[5tgl]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rhizomucor_miehei Rhizomucor miehei]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA].~~	+

	==See Also==		==See Also==
	*[[Lipase\|Lipase]]		*[[Lipase\|Lipase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:002046751<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Rhizomucor miehei]]		[[Category: Rhizomucor miehei]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
-	[[Category: Bjorkling, F.]]	+	[[Category: Bjorkling, F]]
-	[[Category: Brzozowski, A M.]]	+	[[Category: Brzozowski, A M]]
-	[[Category: Derewenda, U.]]	+	[[Category: Derewenda, U]]
-	[[Category: Derewenda, Z S.]]	+	[[Category: Derewenda, Z S]]
-	[[Category: Dodson, G G.]]	+	[[Category: Dodson, G G]]
-	[[Category: Huge-Jensen, B.]]	+	[[Category: Huge-Jensen, B]]
-	[[Category: Lawson, D.]]	+	[[Category: Lawson, D]]
-	[[Category: Patkar, S R.]]	+	[[Category: Patkar, S R]]
-	[[Category: Thim, L.]]	+	[[Category: Thim, L]]
-	[[Category: Turkenburg, J P.]]	+	[[Category: Turkenburg, J P]]

OCA at 05:47, 20 October 2012

2012-10-20T05:47:45Z

←Older revision		Revision as of 05:47, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[5tgl]] is a 1 chain structure ~~of [[Lipase]]~~ with sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA].	+	[[5tgl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA].

	==See Also==		==See Also==

OCA at 11:08, 25 July 2012

2012-07-25T11:08:49Z

←Older revision		Revision as of 11:08, 25 July 2012
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	[[Image:5tgl.png\|left\|200px]]		[[Image:5tgl.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_5tgl", creates the "Structure Box" on the page.
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	{{STRUCTURE_5tgl\| PDB=5tgl \| SCENE= }}		{{STRUCTURE_5tgl\| PDB=5tgl \| SCENE= }}

	===A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX===		===A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX===

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	{{ABSTRACT_PUBMED_2046751}}		{{ABSTRACT_PUBMED_2046751}}

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	==See Also==		==See Also==
-	*[[Lipase]]	+	*[[Lipase\|Lipase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~2046751~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:002046751</ref><references group="xtra"/>
	[[Category: Rhizomucor miehei]]		[[Category: Rhizomucor miehei]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]

OCA: Protected "5tgl" [edit=sysop:move=sysop]

2010-12-26T20:54:02Z

Protected "5tgl" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:54, 26 December 2010

OCA at 20:54, 26 December 2010

2010-12-26T20:54:00Z

←Older revision		Revision as of 20:54, 26 December 2010
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-	{{Seed}}
	[[Image:5tgl.png\|left\|200px]]		[[Image:5tgl.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~5TGL~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA].	+	[[5tgl]] is a 1 chain structure of [[Lipase]] with sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA].
		+
		+	==See Also==
		+	*[[Lipase]]

	==Reference==		==Reference==
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	[[Category: Thim, L.]]		[[Category: Thim, L.]]
	[[Category: Turkenburg, J P.]]		[[Category: Turkenburg, J P.]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:49:35 2009''

OCA at 09:49, 16 February 2009

2009-02-16T09:49:36Z

←Older revision		Revision as of 09:49, 16 February 2009
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	==About this Structure==		==About this Structure==
-	5TGL is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA].	+	5TGL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TGL OCA].

	==Reference==		==Reference==
-	A model for interfacial activation in lipases from the structure of a fungal lipase-inhibitor complex., Brzozowski AM, Derewenda U, Derewenda ZS, Dodson GG, Lawson DM, Turkenburg JP, Bjorkling F, Huge-Jensen B, Patkar SA, Thim L, Nature. 1991 Jun 6;351(6326):491-4. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/2046751 2046751]~~	+	<ref group="xtra">PMID:2046751</ref><references group="xtra"/>
	[[Category: Rhizomucor miehei]]		[[Category: Rhizomucor miehei]]
-	[[Category: Single protein]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Bjorkling, F.]]		[[Category: Bjorkling, F.]]
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	[[Category: Turkenburg, J P.]]		[[Category: Turkenburg, J P.]]

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OCA at 11:11, 3 July 2008

2008-07-03T11:11:29Z

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-	[[Image:5tgl.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:5tgl.png\|left\|200px]]

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	{{STRUCTURE_5tgl\| PDB=5tgl \| SCENE= }}		{{STRUCTURE_5tgl\| PDB=5tgl \| SCENE= }}

-	~~'''~~A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX~~'''~~	+	===A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX===


-	~~==Overview==~~	+	<!--
-	~~Lipases are hydrolytic enzymes which break down triacylglycerides into free fatty acids and glycerols. They have been classified as serine hydrolases owing to their inhibition by diethyl p~~-~~nitrophenyl phosphate. Lipase activity is greatly increased at the lipid~~-~~water interface~~, ~~a phenomenon known as interfacial activation. X-ray analysis has revealed the atomic structures of two triacylglycerol lipases~~, ~~unrelated in sequence:~~ the ~~human pancreatic lipase (hPL)4, and an enzyme isolated from~~ the ~~fungus Rhizomucor~~ (~~formerly Mucor) miehei (RmL)~~. ~~In both enzymes the active centres contain structurally analogous Asp-His-Ser triads (characteristic of serine proteinases~~), which are buried completely beneath a short helical segment, or 'lid'. Here we present the crystal structure (at 3 A resolution) of a complex of R. miehei lipase with n-hexylphosphonate ethyl ester in which the enzyme's active site is ~~exposed by~~ the ~~movement of the helical lid~~. This movement also increases the nonpolarity of the surface surrounding the catalytic site. We propose that the structure of the enzyme in this complex is equivalent to the activated state generated by the oil-~~water interface.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_2046751}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 2046751 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_2046751}}

	==About this Structure==		==About this Structure==
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	[[Category: Thim, L.]]		[[Category: Thim, L.]]
	[[Category: Turkenburg, J P.]]		[[Category: Turkenburg, J P.]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:37:51 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 14:11:29 2008''