5tj8 - Revision history

OCA at 13:03, 4 October 2023

2023-10-04T13:03:16Z

←Older revision		Revision as of 13:03, 4 October 2023
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	==Structure of WWP2 WW2-2,3-linker-HECT (no WW2 observed)==		==Structure of WWP2 WW2-2,3-linker-HECT (no WW2 observed)==
-	<StructureSection load='5tj8' size='340' side='right' caption='[[5tj8]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5tj8' size='340' side='right'caption='[[5tj8]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5tj8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TJ8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5TJ8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5tj8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TJ8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tj7\|5tj7]]~~, [[~~5tjq~~\|~~5tjq~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~WWP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tj8 OCA], [https://pdbe.org/5tj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tj8 RCSB], [https://www.ebi.ac.uk/pdbsum/5tj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tj8 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5tj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tj8 OCA], [~~http~~://pdbe.org/5tj8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5tj8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5tj8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5tj8 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/WWP2_HUMAN WWP2_HUMAN]] E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.<ref>PMID:19274063</ref> <ref>PMID:19651900</ref>	+	[https://www.uniprot.org/uniprot/WWP2_HUMAN WWP2_HUMAN] E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.<ref>PMID:19274063</ref> <ref>PMID:19651900</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5tj8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5tj8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ubiquitin protein ligase 3D structures\|Ubiquitin protein ligase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Chen, Z~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gabelli, S B~~]]	+	[[Category: Chen Z]]
-	[[Category: ~~Autoinhibition]]~~	+	[[Category: Gabelli SB]]
-	~~[[Category: Hect domain]]~~	+
-	~~[[Category: Itch]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Ww2]]~~	+
-	~~[[Category: Wwp1]]~~	+
-	~~[[Category: Wwp2~~]]	+

OCA at 10:55, 16 November 2017

2017-11-16T10:55:19Z

←Older revision		Revision as of 10:55, 16 November 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5tj8 is ~~ON HOLD~~	+	==Structure of WWP2 WW2-2,3-linker-HECT (no WW2 observed)==
		+	<StructureSection load='5tj8' size='340' side='right' caption='[[5tj8]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5tj8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TJ8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5TJ8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5tj7\|5tj7]], [[5tjq\|5tjq]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">WWP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5tj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tj8 OCA], [http://pdbe.org/5tj8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tj8 RCSB], [http://www.ebi.ac.uk/pdbsum/5tj8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tj8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/WWP2_HUMAN WWP2_HUMAN]] E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.<ref>PMID:19274063</ref> <ref>PMID:19651900</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The HECT E3 ligases ubiquitinate numerous transcription factors and signaling molecules, and their activity must be tightly controlled to prevent cancer, immune disorders, and other diseases. In this study, we have found unexpectedly that peptide linkers tethering WW domains in several HECT family members are key regulatory elements of their catalytic activities. Biochemical, structural, and cellular analyses have revealed that the linkers can lock the HECT domain in an inactive conformation and block the proposed allosteric ubiquitin binding site. Such linker-mediated autoinhibition of the HECT domain can be relieved by linker post-translational modifications, but complete removal of the brake can induce hyperactive autoubiquitination and E3 self destruction. These results clarify the mechanisms of several HECT protein cancer associated mutations and provide a new framework for understanding how HECT ubiquitin ligases must be finely tuned to ensure normal cellular behavior.

-	~~Authors: Chen~~, Z., Gabelli, S.B.	+	A Tunable Brake for HECT Ubiquitin Ligases.,Chen Z, Jiang H, Xu W, Li X, Dempsey DR, Zhang X, Devreotes P, Wolberger C, Amzel LM, Gabelli SB, Cole PA Mol Cell. 2017 May 4;66(3):345-357.e6. doi: 10.1016/j.molcel.2017.03.020. PMID:28475870<ref>PMID:28475870</ref>

-	~~Description: STRUCTURE OF HECT_27 .~~...	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5tj8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human]]
	[[Category: Chen, Z]]		[[Category: Chen, Z]]
-	[[Category: Gabelli, S.B]]	+	[[Category: Gabelli, S B]]
		+	[[Category: Autoinhibition]]
		+	[[Category: Hect domain]]
		+	[[Category: Itch]]
		+	[[Category: Transferase]]
		+	[[Category: Ww2]]
		+	[[Category: Wwp1]]
		+	[[Category: Wwp2]]

OCA: Protected "5tj8" [edit=sysop:move=sysop]

2016-10-19T15:54:47Z

Protected "5tj8" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:54, 19 October 2016

OCA: New page: '''Unreleased structure''' The entry 5tj8 is ON HOLD Authors: Chen, Z., Gabelli, S.B. Description: STRUCTURE OF HECT_27 .... Category: Unreleased Structures Category: Chen, Z [...

2016-10-19T15:54:46Z

New page: '''Unreleased structure''' The entry 5tj8 is ON HOLD Authors: Chen, Z., Gabelli, S.B. Description: STRUCTURE OF HECT_27 .... Category: Unreleased Structures Category: Chen, Z [...

New page

'''Unreleased structure'''

The entry 5tj8 is ON HOLD

Authors: Chen, Z., Gabelli, S.B.

Description: STRUCTURE OF HECT_27 ....
[[Category: Unreleased Structures]]
[[Category: Chen, Z]]
[[Category: Gabelli, S.B]]