5u1y - Revision history

OCA at 15:44, 6 March 2024

2024-03-06T15:44:28Z

←Older revision		Revision as of 15:44, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='5u1y' size='340' side='right'caption='[[5u1y]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='5u1y' size='340' side='right'caption='[[5u1y]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u1y]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ailme Ailme~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5U1Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u1y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ailuropoda_melanoleuca Ailuropoda melanoleuca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U1Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7RS:N~2~-(3,4-DIFLUOROPHENYL)-N-{2-METHYL-5-[(PIPERAZIN-1-YL)METHYL]PHENYL}GLYCINAMIDE'>7RS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u1l\|5u1l]], [[5u1u\|5u1u]], [[5u1v\|5u1v]], [[5u1w\|5u1w]], [[5u1x\|5u1x]], [[5u2h\|5u2h]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7RS:N~2~-(3,4-DIFLUOROPHENYL)-N-{2-METHYL-5-[(PIPERAZIN-1-YL)METHYL]PHENYL}GLYCINAMIDE'>7RS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P2RX7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9646 AILME])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u1y OCA], [https://pdbe.org/5u1y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u1y RCSB], [https://www.ebi.ac.uk/pdbsum/5u1y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u1y ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5u1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u1y OCA], [~~http~~://pdbe.org/5u1y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5u1y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5u1y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5u1y ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/G1M6C4_AILME G1M6C4_AILME]] Receptor for ATP that acts as a ligand-gated ion channel.[RuleBase:RU000681]	+	[https://www.uniprot.org/uniprot/G1M6C4_AILME G1M6C4_AILME] Receptor for ATP that acts as a ligand-gated ion channel.[RuleBase:RU000681]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The P2X7 receptor is a non-selective cation channel activated by extracellular adenosine triphosphate (ATP). Chronic activation of P2X7 underlies many health problems such as pathologic pain, yet we lack effective antagonists due to poorly understood mechanisms of inhibition. Here we present crystal structures of a mammalian P2X7 receptor complexed with five structurally-unrelated antagonists. Unexpectedly, these drugs all bind to an allosteric site distinct from the ATP-binding pocket in a groove formed between two neighboring subunits. This novel drug-binding pocket accommodates a diversity of small molecules mainly through hydrophobic interactions. Functional assays propose that these compounds allosterically prevent narrowing of the drug-binding pocket and the turret-like architecture during channel opening, which is consistent with a site of action distal to the ATP-binding pocket. These novel mechanistic insights will facilitate the development of P2X7-specific drugs for treating human diseases.	+

-	~~Structural basis for subtype-specific inhibition of the P2X7 receptor.,Karasawa A, Kawate T Elife. 2016 Dec 9;5. pii: e22153. doi: 10.7554/eLife.22153. PMID:27935479<ref>PMID:27935479</ref>~~	+	==See Also==
-		+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5u1y" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ailme~~]]	+	[[Category: Ailuropoda melanoleuca]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Karasawa, A]]	+	[[Category: Karasawa A]]
-	[[Category: Kawate, T]]	+	[[Category: Kawate T]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Membrane protein: atp-gated ion channel: allosteric antagonist bound: closed state~~]]	+

OCA at 09:04, 18 December 2019

2019-12-18T09:04:46Z

←Older revision		Revision as of 09:04, 18 December 2019
Line 1:		Line 1:

	==Crystal structure of the ATP-gated P2X7 ion channel bound to allosteric antagonist GW791343==		==Crystal structure of the ATP-gated P2X7 ion channel bound to allosteric antagonist GW791343==
-	<StructureSection load='5u1y' size='340' side='right' caption='[[5u1y]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='5u1y' size='340' side='right'caption='[[5u1y]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5u1y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ailme Ailme]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U1Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[5u1y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ailme Ailme]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U1Y FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Ailme]]		[[Category: Ailme]]
		+	[[Category: Large Structures]]
	[[Category: Karasawa, A]]		[[Category: Karasawa, A]]
	[[Category: Kawate, T]]		[[Category: Kawate, T]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Membrane protein: atp-gated ion channel: allosteric antagonist bound: closed state]]		[[Category: Membrane protein: atp-gated ion channel: allosteric antagonist bound: closed state]]

OCA at 19:24, 15 November 2017

2017-11-15T19:24:16Z

←Older revision		Revision as of 19:24, 15 November 2017
Line 3:		Line 3:
	<StructureSection load='5u1y' size='340' side='right' caption='[[5u1y]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='5u1y' size='340' side='right' caption='[[5u1y]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5u1y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U1Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5u1y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ailme Ailme]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U1Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7RS:N~2~-(3,4-DIFLUOROPHENYL)-N-{2-METHYL-5-[(PIPERAZIN-1-YL)METHYL]PHENYL}GLYCINAMIDE'>7RS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7RS:N~2~-(3,4-DIFLUOROPHENYL)-N-{2-METHYL-5-[(PIPERAZIN-1-YL)METHYL]PHENYL}GLYCINAMIDE'>7RS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u1l\|5u1l]], [[5u1u\|5u1u]], [[5u1v\|5u1v]], [[5u1w\|5u1w]], [[5u1x\|5u1x]], [[5u2h\|5u2h]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u1l\|5u1l]], [[5u1u\|5u1u]], [[5u1v\|5u1v]], [[5u1w\|5u1w]], [[5u1x\|5u1x]], [[5u2h\|5u2h]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">P2RX7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9646 AILME])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u1y OCA], [http://pdbe.org/5u1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u1y RCSB], [http://www.ebi.ac.uk/pdbsum/5u1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u1y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u1y OCA], [http://pdbe.org/5u1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u1y RCSB], [http://www.ebi.ac.uk/pdbsum/5u1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u1y ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ailme]]
	[[Category: Karasawa, A]]		[[Category: Karasawa, A]]
	[[Category: Kawate, T]]		[[Category: Kawate, T]]
	[[Category: Membrane protein]]		[[Category: Membrane protein]]
	[[Category: Membrane protein: atp-gated ion channel: allosteric antagonist bound: closed state]]		[[Category: Membrane protein: atp-gated ion channel: allosteric antagonist bound: closed state]]

OCA at 16:42, 4 January 2017

2017-01-04T16:42:00Z

←Older revision		Revision as of 16:42, 4 January 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5u1y is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of the ATP-gated P2X7 ion channel bound to allosteric antagonist GW791343==
		+	<StructureSection load='5u1y' size='340' side='right' caption='[[5u1y]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5u1y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U1Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U1Y FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7RS:N~2~-(3,4-DIFLUOROPHENYL)-N-{2-METHYL-5-[(PIPERAZIN-1-YL)METHYL]PHENYL}GLYCINAMIDE'>7RS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5u1l\|5u1l]], [[5u1u\|5u1u]], [[5u1v\|5u1v]], [[5u1w\|5u1w]], [[5u1x\|5u1x]], [[5u2h\|5u2h]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u1y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u1y OCA], [http://pdbe.org/5u1y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u1y RCSB], [http://www.ebi.ac.uk/pdbsum/5u1y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u1y ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/G1M6C4_AILME G1M6C4_AILME]] Receptor for ATP that acts as a ligand-gated ion channel.[RuleBase:RU000681]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The P2X7 receptor is a non-selective cation channel activated by extracellular adenosine triphosphate (ATP). Chronic activation of P2X7 underlies many health problems such as pathologic pain, yet we lack effective antagonists due to poorly understood mechanisms of inhibition. Here we present crystal structures of a mammalian P2X7 receptor complexed with five structurally-unrelated antagonists. Unexpectedly, these drugs all bind to an allosteric site distinct from the ATP-binding pocket in a groove formed between two neighboring subunits. This novel drug-binding pocket accommodates a diversity of small molecules mainly through hydrophobic interactions. Functional assays propose that these compounds allosterically prevent narrowing of the drug-binding pocket and the turret-like architecture during channel opening, which is consistent with a site of action distal to the ATP-binding pocket. These novel mechanistic insights will facilitate the development of P2X7-specific drugs for treating human diseases.

-	~~Authors~~:	+	Structural basis for subtype-specific inhibition of the P2X7 receptor.,Karasawa A, Kawate T Elife. 2016 Dec 9;5. pii: e22153. doi: 10.7554/eLife.22153. PMID:27935479<ref>PMID:27935479</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5u1y" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Karasawa, A]]
		+	[[Category: Kawate, T]]
		+	[[Category: Membrane protein]]
		+	[[Category: Membrane protein: atp-gated ion channel: allosteric antagonist bound: closed state]]

OCA at 17:33, 15 December 2016

2016-12-15T17:33:54Z

←Older revision		Revision as of 17:33, 15 December 2016
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5u1y is ON HOLD	+	The entry 5u1y is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5u1y" [edit=sysop:move=sysop]

2016-12-09T18:11:54Z

Protected "5u1y" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:11, 9 December 2016

OCA: New page: '''Unreleased structure''' The entry 5u1y is ON HOLD Authors: Description: Category: Unreleased Structures

2016-12-09T18:11:53Z

New page: '''Unreleased structure''' The entry 5u1y is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5u1y is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]