5uc1 - Revision history

OCA at 13:24, 4 October 2023

2023-10-04T13:24:53Z

←Older revision		Revision as of 13:24, 4 October 2023
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	==Structural Analysis of Glucocorticoid Receptor beta Ligand Binding Domain Complexed with Glucocorticoid Antagonist RU-486: Implication of Helix 12 in Antagonism==		==Structural Analysis of Glucocorticoid Receptor beta Ligand Binding Domain Complexed with Glucocorticoid Antagonist RU-486: Implication of Helix 12 in Antagonism==
-	<StructureSection load='5uc1' size='340' side='right' caption='[[5uc1]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='5uc1' size='340' side='right'caption='[[5uc1]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uc1]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hetga Hetga~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UC1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uc1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Heterocephalus_glaber Heterocephalus glaber]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UC1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UC1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=486:11-(4-DIMETHYLAMINO-PHENYL)-17-HYDROXY-13-METHYL-17-PROP-1-YNYL-1,2,6,7,8,11,12,13,14,15,16,17-DODEC+AHYDRO-CYCLOPENTA[A]PHENANTHREN-3-ONE'>486</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.351Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uc3\|5uc3]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=486:11-(4-DIMETHYLAMINO-PHENYL)-17-HYDROXY-13-METHYL-17-PROP-1-YNYL-1,2,6,7,8,11,12,13,14,15,16,17-DODEC+AHYDRO-CYCLOPENTA[A]PHENANTHREN-3-ONE'>486</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GW7_10599 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10181 HETGA])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uc1 OCA], [https://pdbe.org/5uc1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uc1 RCSB], [https://www.ebi.ac.uk/pdbsum/5uc1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uc1 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5uc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uc1 OCA], [~~http~~://pdbe.org/5uc1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5uc1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5uc1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5uc1 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G5AQS2_HETGA G5AQS2_HETGA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:

	==See Also==		==See Also==
-	*[[Glucocorticoid receptor\|Glucocorticoid receptor]]	+	*[[Glucocorticoid receptor 3D structures\|Glucocorticoid receptor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hetga~~]]	+	[[Category: Heterocephalus glaber]]
-	[[Category: ~~Min, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Pedersen, L C~~]]	+	[[Category: Min J]]
-	[[Category: ~~Hormone receptor]]~~	+	[[Category: Pedersen LC]]
-	~~[[Category: Nuclear receptor~~]]	+

OCA at 06:19, 20 June 2018

2018-06-20T06:19:11Z

←Older revision		Revision as of 06:19, 20 June 2018
Line 3:		Line 3:
	<StructureSection load='5uc1' size='340' side='right' caption='[[5uc1]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='5uc1' size='340' side='right' caption='[[5uc1]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uc1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UC1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uc1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Hetga Hetga]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UC1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=486:11-(4-DIMETHYLAMINO-PHENYL)-17-HYDROXY-13-METHYL-17-PROP-1-YNYL-1,2,6,7,8,11,12,13,14,15,16,17-DODEC+AHYDRO-CYCLOPENTA[A]PHENANTHREN-3-ONE'>486</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=486:11-(4-DIMETHYLAMINO-PHENYL)-17-HYDROXY-13-METHYL-17-PROP-1-YNYL-1,2,6,7,8,11,12,13,14,15,16,17-DODEC+AHYDRO-CYCLOPENTA[A]PHENANTHREN-3-ONE'>486</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uc3\|5uc3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uc3\|5uc3]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GW7_10599 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10181 HETGA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uc1 OCA], [http://pdbe.org/5uc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uc1 RCSB], [http://www.ebi.ac.uk/pdbsum/5uc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uc1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uc1 OCA], [http://pdbe.org/5uc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uc1 RCSB], [http://www.ebi.ac.uk/pdbsum/5uc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uc1 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glucocorticoid receptor beta (GRbeta) is associated with glucocorticoid resistance via dominant negative regulation of GRalpha. To better understand how GRbeta functions as a dominant negative inhibitor of GRalpha at a molecular level, we determined the crystal structure of the ligand binding domain of GRbeta complexed with antagonist RU-486. The structure reveals that RU-486 binds in the same ligand binding pocket as in GRalpha and the unique C-terminal amino acids of GRbeta are mostly disordered. Binding energy analysis suggests that these C-terminal residues of GRbeta do not contribute to RU-486 binding. Intriguingly, the GRbeta/RU-486 complex binds corepressor peptide with similar affinity as GRalpha/RU-486 complex, despite the lack of helix 12. Our biophysical and biochemical analysis reveals that in the presence of RU-486, GRbeta is found in a conformation that favors corepressor binding, potentially antagonizing GRalpha function. This study thus presents an unexpected molecular mechanism by which GRbeta could repress transcription.
		+
		+	Probing Dominant Negative Behavior of Glucocorticoid Receptor beta through a Hybrid Structural and Biochemical Approach.,Min J, Perera L, Krahn JM, Jewell CM, Moon AF, Cidlowski JA, Pedersen LC Mol Cell Biol. 2018 Feb 5. pii: MCB.00453-17. doi: 10.1128/MCB.00453-17. PMID:29437838<ref>PMID:29437838</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5uc1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucocorticoid receptor\|Glucocorticoid receptor]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hetga]]
	[[Category: Min, J]]		[[Category: Min, J]]
	[[Category: Pedersen, L C]]		[[Category: Pedersen, L C]]
	[[Category: Hormone receptor]]		[[Category: Hormone receptor]]
	[[Category: Nuclear receptor]]		[[Category: Nuclear receptor]]

OCA at 08:26, 27 December 2017

2017-12-27T08:26:47Z

←Older revision		Revision as of 08:26, 27 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5uc1 is ~~ON HOLD until Paper Publication~~	+	==Structural Analysis of Glucocorticoid Receptor beta Ligand Binding Domain Complexed with Glucocorticoid Antagonist RU-486: Implication of Helix 12 in Antagonism==
-		+	<StructureSection load='5uc1' size='340' side='right' caption='[[5uc1]], [[Resolution\|resolution]] 2.35Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5uc1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UC1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UC1 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=486:11-(4-DIMETHYLAMINO-PHENYL)-17-HYDROXY-13-METHYL-17-PROP-1-YNYL-1,2,6,7,8,11,12,13,14,15,16,17-DODEC+AHYDRO-CYCLOPENTA[A]PHENANTHREN-3-ONE'>486</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uc3\|5uc3]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uc1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uc1 OCA], [http://pdbe.org/5uc1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uc1 RCSB], [http://www.ebi.ac.uk/pdbsum/5uc1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uc1 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Min, J]]
		+	[[Category: Pedersen, L C]]
		+	[[Category: Hormone receptor]]
		+	[[Category: Nuclear receptor]]

OCA at 14:20, 9 March 2017

2017-03-09T14:20:38Z

←Older revision		Revision as of 14:20, 9 March 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5uc1 is ON HOLD	+	The entry 5uc1 is ON HOLD until Paper Publication

-	Authors: ~~Pedersen, L.C., Min, J.~~	+	Authors:

-	Description: ~~Nuclear receptor~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Min, J]]
-	[[Category: Pedersen, L.C]]

OCA at 14:40, 18 January 2017

2017-01-18T14:40:17Z

←Older revision		Revision as of 14:40, 18 January 2017
Line 3:		Line 3:
	The entry 5uc1 is ON HOLD		The entry 5uc1 is ON HOLD

-	Authors: Pedersen, L.C., Min, J	+	Authors: Pedersen, L.C., Min, J.

	Description: Nuclear receptor		Description: Nuclear receptor

OCA: Protected "5uc1" [edit=sysop:move=sysop]

2017-01-02T15:20:45Z

Protected "5uc1" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:20, 2 January 2017

OCA: New page: '''Unreleased structure''' The entry 5uc1 is ON HOLD Authors: Pedersen, L.C., Min, J Description: Nuclear receptor Category: Unreleased Structures Category: Min, J [[Category: ...

2017-01-02T15:20:44Z

New page: '''Unreleased structure''' The entry 5uc1 is ON HOLD Authors: Pedersen, L.C., Min, J Description: Nuclear receptor Category: Unreleased Structures Category: Min, J [[Category: ...

New page

'''Unreleased structure'''

The entry 5uc1 is ON HOLD

Authors: Pedersen, L.C., Min, J

Description: Nuclear receptor
[[Category: Unreleased Structures]]
[[Category: Min, J]]
[[Category: Pedersen, L.C]]