5uwu - Revision history

OCA at 13:36, 4 October 2023

2023-10-04T13:36:30Z

←Older revision		Revision as of 13:36, 4 October 2023
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	<StructureSection load='5uwu' size='340' side='right'caption='[[5uwu]], [[Resolution\|resolution]] 2.24Å' scene=''>		<StructureSection load='5uwu' size='340' side='right'caption='[[5uwu]], [[Resolution\|resolution]] 2.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uwu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UWU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uwu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UWU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.24Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uwi\|5uwi]], [[5uwh\|5uwh]], [[5uwj\|5uwj]], [[5uwo\|5uwo]], [[5uwp\|5uwp]], [[5uwq\|5uwq]], [[5uwr\|5uwr]], [[5uws\|5uws]], [[5uwt\|5uwt]], [[5uww\|5uww]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RAN, ARA24, OK/SW-cl.81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), YRB1, CST20, HTN1, SFO1, YDR002W, YD8119.08 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), CRM1, KAP124, XPO1, YGR218W, G8514 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SMAD4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uwu OCA], [https://pdbe.org/5uwu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uwu RCSB], [https://www.ebi.ac.uk/pdbsum/5uwu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uwu ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5uwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uwu OCA], [~~http~~://pdbe.org/5uwu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5uwu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5uwu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5uwu ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> [[http://www.uniprot.org/uniprot/XPO1_YEAST XPO1_YEAST]] Receptor for the leucine-rich nuclear export signal (NES). [[http://www.uniprot.org/uniprot/YRB1_YEAST YRB1_YEAST]] Important for the export of protein containing nuclear export signal (NES) out of the nucleus. Stimulates the GTPase activity of GSP1 and GSP2.	+	[https://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 18824]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chook, Y M~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Fung, H Y.J~~]]	+	[[Category: Chook YM]]
-	[[Category: ~~Heat repeat]]~~	+	[[Category: Fung HYJ]]
-	~~[[Category: Karyopherin]]~~	+
-	~~[[Category: Ne]]~~	+
-	~~[[Category: Nuclear export]]~~	+
-	~~[[Category: Protein transport~~]]	+

OCA at 16:46, 20 November 2019

2019-11-20T16:46:15Z

←Older revision		Revision as of 16:46, 20 November 2019
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Exportin\|Exportin]]	+	*[[Exportin 3D structures\|Exportin 3D structures]]
-	*[[GTP-binding protein\|GTP-binding protein]]	+	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:41, 24 April 2019

2019-04-24T07:41:10Z

←Older revision		Revision as of 07:41, 24 April 2019
Line 1:		Line 1:

	==Crystal Structure of SMAD4 NES Peptide in complex with CRM1-Ran-RanBP1==		==Crystal Structure of SMAD4 NES Peptide in complex with CRM1-Ran-RanBP1==
-	<StructureSection load='5uwu' size='340' side='right' caption='[[5uwu]], [[Resolution\|resolution]] 2.24Å' scene=''>	+	<StructureSection load='5uwu' size='340' side='right'caption='[[5uwu]], [[Resolution\|resolution]] 2.24Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uwu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UWU FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uwu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UWU FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 5uwu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5uwu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Exportin\|Exportin]]
		+	*[[GTP-binding protein\|GTP-binding protein]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 30:
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Chook, Y M]]		[[Category: Chook, Y M]]
	[[Category: Fung, H Y.J]]		[[Category: Fung, H Y.J]]

OCA at 14:37, 6 November 2017

2017-11-06T14:37:59Z

←Older revision		Revision as of 14:37, 6 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5uwu is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal Structure of SMAD4 NES Peptide in complex with CRM1-Ran-RanBP1==
		+	<StructureSection load='5uwu' size='340' side='right' caption='[[5uwu]], [[Resolution\|resolution]] 2.24Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5uwu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UWU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uwi\|5uwi]], [[5uwh\|5uwh]], [[5uwj\|5uwj]], [[5uwo\|5uwo]], [[5uwp\|5uwp]], [[5uwq\|5uwq]], [[5uwr\|5uwr]], [[5uws\|5uws]], [[5uwt\|5uwt]], [[5uww\|5uww]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RAN, ARA24, OK/SW-cl.81 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), YRB1, CST20, HTN1, SFO1, YDR002W, YD8119.08 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), CRM1, KAP124, XPO1, YGR218W, G8514 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SMAD4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uwu OCA], [http://pdbe.org/5uwu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uwu RCSB], [http://www.ebi.ac.uk/pdbsum/5uwu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uwu ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN]] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> [[http://www.uniprot.org/uniprot/XPO1_YEAST XPO1_YEAST]] Receptor for the leucine-rich nuclear export signal (NES). [[http://www.uniprot.org/uniprot/YRB1_YEAST YRB1_YEAST]] Important for the export of protein containing nuclear export signal (NES) out of the nucleus. Stimulates the GTPase activity of GSP1 and GSP2.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nuclear export receptor CRM1 binds highly variable nuclear export signals (NESs) in hundreds of different cargoes. Previously we have shown that CRM1 binds NESs in both polypeptide orientations (Fung et al., 2015). Here, we show crystal structures of CRM1 bound to eight additional NESs which reveal diverse conformations that range from loop-like to all-helix, which occupy different extents of the invariant NES-binding groove. Analysis of all NES structures show 5-6 distinct backbone conformations where the only conserved secondary structural element is one turn of helix that binds the central portion of the CRM1 groove. All NESs also participate in main chain hydrogen bonding with human CRM1 Lys568 side chain, which acts as a specificity filter that prevents binding of non-NES peptides. The large conformational range of NES backbones explains the lack of a fixed pattern for its 3-5 hydrophobic anchor residues, which in turn explains the large array of peptide sequences that can function as NESs.

-	~~Authors:~~ Fung, H.Y.J.~~, Chook, Y~~.M.	+	Nuclear export receptor CRM1 recognizes diverse conformations in nuclear export signals.,Fung HY, Fu SC, Chook YM Elife. 2017 Mar 10;6. pii: e23961. doi: 10.7554/eLife.23961. PMID:28282025<ref>PMID:28282025</ref>

-	~~Description: Crystal Structure~~ of ~~SMAD4 NES Peptide in complex with CRM1~~-~~Ran~~-~~RanBP1~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Chook, Y.M]]	+	<div class="pdbe-citations 5uwu" style="background-color:#fffaf0;"></div>
-	[[Category: Fung, H.Y.J]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Atcc 18824]]
		+	[[Category: Human]]
		+	[[Category: Chook, Y M]]
		+	[[Category: Fung, H Y.J]]
		+	[[Category: Heat repeat]]
		+	[[Category: Karyopherin]]
		+	[[Category: Ne]]
		+	[[Category: Nuclear export]]
		+	[[Category: Protein transport]]

OCA at 19:27, 15 March 2017

2017-03-15T19:27:54Z

←Older revision		Revision as of 19:27, 15 March 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5uwu is ON HOLD	+	The entry 5uwu is ON HOLD until Paper Publication

	Authors: Fung, H.Y.J., Chook, Y.M.		Authors: Fung, H.Y.J., Chook, Y.M.

OCA: Protected "5uwu" [edit=sysop:move=sysop]

2017-03-09T15:02:17Z

Protected "5uwu" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:02, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5uwu is ON HOLD Authors: Fung, H.Y.J., Chook, Y.M. Description: Crystal Structure of SMAD4 NES Peptide in complex with CRM1-Ran-RanBP1 [[Category: ...

2017-03-09T15:02:16Z

New page: '''Unreleased structure''' The entry 5uwu is ON HOLD Authors: Fung, H.Y.J., Chook, Y.M. Description: Crystal Structure of SMAD4 NES Peptide in complex with CRM1-Ran-RanBP1 [[Category: ...

New page

'''Unreleased structure'''

The entry 5uwu is ON HOLD

Authors: Fung, H.Y.J., Chook, Y.M.

Description: Crystal Structure of SMAD4 NES Peptide in complex with CRM1-Ran-RanBP1
[[Category: Unreleased Structures]]
[[Category: Chook, Y.M]]
[[Category: Fung, H.Y.J]]