5uwv - Revision history

OCA at 13:36, 4 October 2023

2023-10-04T13:36:36Z

←Older revision		Revision as of 13:36, 4 October 2023
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	<StructureSection load='5uwv' size='340' side='right'caption='[[5uwv]], [[Resolution\|resolution]] 2.98Å' scene=''>		<StructureSection load='5uwv' size='340' side='right'caption='[[5uwv]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uwv]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myca9 Myca9~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UWV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uwv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacteroides_abscessus_ATCC_19977 Mycobacteroides abscessus ATCC 19977]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UWV FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~MAB_1530 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=561007 MYCA9])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.98Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5uwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uwv OCA], [~~http~~://pdbe.org/5uwv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5uwv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5uwv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5uwv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uwv OCA], [https://pdbe.org/5uwv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uwv RCSB], [https://www.ebi.ac.uk/pdbsum/5uwv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uwv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B1MMQ4_MYCA9 B1MMQ4_MYCA9]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myca9~~]]	+	[[Category: Mycobacteroides abscessus ATCC 19977]]
-	[[Category: Ginell~~, S L~~]]	+	[[Category: Ginell SL]]
-	[[Category: Kumar, P]]	+	[[Category: Kumar P]]
-	[[Category: Lamichhane, G]]	+	[[Category: Lamichhane G]]
-	~~[[Category: Cell wall enzyme]]~~	+
-	~~[[Category: D-transpeptidase]]~~	+
-	~~[[Category: Ldtmab2]]~~	+
-	~~[[Category: Mycobacterium abscessus]]~~	+
-	~~[[Category: Peptidoglycan synthesis enzyme]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 17:13, 14 August 2019

2019-08-14T17:13:48Z

←Older revision		Revision as of 17:13, 14 August 2019
Line 1:		Line 1:

	==Crystal structure of Mycobacterium abscessus L,D-transpeptidase 2==		==Crystal structure of Mycobacterium abscessus L,D-transpeptidase 2==
-	<StructureSection load='5uwv' size='340' side='right' caption='[[5uwv]], [[Resolution\|resolution]] 2.98Å' scene=''>	+	<StructureSection load='5uwv' size='340' side='right'caption='[[5uwv]], [[Resolution\|resolution]] 2.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uwv]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UWV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uwv]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Myca9 Myca9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UWV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uwv OCA], [http://pdbe.org/5uwv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uwv RCSB], [http://www.ebi.ac.uk/pdbsum/5uwv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uwv ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MAB_1530 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=561007 MYCA9])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uwv OCA], [http://pdbe.org/5uwv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uwv RCSB], [http://www.ebi.ac.uk/pdbsum/5uwv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uwv ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ginell, L S]]	+	[[Category: Large Structures]]
		+	[[Category: Myca9]]
		+	[[Category: Ginell, S L]]
	[[Category: Kumar, P]]		[[Category: Kumar, P]]
	[[Category: Lamichhane, G]]		[[Category: Lamichhane, G]]

OCA at 05:57, 17 August 2017

2017-08-17T05:57:23Z

←Older revision		Revision as of 05:57, 17 August 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 5uwv is ~~ON HOLD~~	+	==Crystal structure of Mycobacterium abscessus L,D-transpeptidase 2==
		+	<StructureSection load='5uwv' size='340' side='right' caption='[[5uwv]], [[Resolution\|resolution]] 2.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5uwv]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UWV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UWV FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uwv OCA], [http://pdbe.org/5uwv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uwv RCSB], [http://www.ebi.ac.uk/pdbsum/5uwv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uwv ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	As a growing number of clinical isolates of Mycobacterium abscessus are resistant to most antibiotics, new treatment options that are effective against these drug-resistant strains are desperately needed. The majority of the linkages in the cell wall peptidoglycan of M. abscessus are synthesized by non-classical transpeptidases, namely the L,D-transpeptidases. Emerging evidence suggests that these enzymes represent a new molecular vulnerability in this pathogen. Recent studies have demonstrated that inhibition of these enzymes by the carbapenem class of beta-lactams determines their activity against M. tuberculosis Here, we studied the interactions of beta-lactams with two L,D-transpeptidases in M. abscessus, namely LdtMab1 and LdtMab2, and found both the carbapenem and cephalosporin--but not penicillin-sub-class of beta-lactams inhibit these enzymes. Contrary to the commonly held belief that combination therapy with beta-lactams is redundant, doripenem and cefdinir exhibit synergy against both pan-susceptible M. abscessus and clinical isolates that are resistant to most antibiotics, which suggests that dual-beta-lactam therapy holds potential for treatment of M. abscessus Lastly, we solved the first crystal structure of an M. abscessus L,D-transpeptidase, LdtMab2, and using substitutions of critical amino acids in the catalytic site and computational simulations, we describe the key molecular interactions between this enzyme and beta-lactams, which provide an insight into the molecular basis for the relative efficacy of different beta-lactams against M. abscessus.

-	~~Authors: Kumar~~, P., Ginell, L.S., ~~Lamichhane~~, G.	+	Mycobacterium abscessus L,D-transpeptidases are susceptible to inactivation by carbapenems and cephalosporins but not penicillins.,Kumar P, Chauhan V, Silva JRA, Lameira J, d'Andrea F, Li S, Ginell SL, Freundlich JS, Alves CN, Bailey S, Cohen KA, Lamichhane G Antimicrob Agents Chemother. 2017 Jul 31. pii: AAC.00866-17. doi:, 10.1128/AAC.00866-17. PMID:28760902<ref>PMID:28760902</ref>

-	~~Description: Crystal structure of Mycobacterium abscessus L~~,~~D-transpeptidase 2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Ginell, L.S]]	+	<div class="pdbe-citations 5uwv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ginell, L S]]
	[[Category: Kumar, P]]		[[Category: Kumar, P]]
	[[Category: Lamichhane, G]]		[[Category: Lamichhane, G]]
		+	[[Category: Cell wall enzyme]]
		+	[[Category: D-transpeptidase]]
		+	[[Category: Ldtmab2]]
		+	[[Category: Mycobacterium abscessus]]
		+	[[Category: Peptidoglycan synthesis enzyme]]
		+	[[Category: Transferase]]

OCA: Protected "5uwv" [edit=sysop:move=sysop]

2017-03-09T15:02:21Z

Protected "5uwv" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:02, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5uwv is ON HOLD Authors: Kumar, P., Ginell, L.S., Lamichhane, G. Description: Crystal structure of Mycobacterium abscessus L,D-transpeptidase 2 [[C...

2017-03-09T15:02:19Z

New page: '''Unreleased structure''' The entry 5uwv is ON HOLD Authors: Kumar, P., Ginell, L.S., Lamichhane, G. Description: Crystal structure of Mycobacterium abscessus L,D-transpeptidase 2 [[C...

New page

'''Unreleased structure'''

The entry 5uwv is ON HOLD

Authors: Kumar, P., Ginell, L.S., Lamichhane, G.

Description: Crystal structure of Mycobacterium abscessus L,D-transpeptidase 2
[[Category: Unreleased Structures]]
[[Category: Ginell, L.S]]
[[Category: Kumar, P]]
[[Category: Lamichhane, G]]