5uxm - Revision history

OCA at 13:36, 4 October 2023

2023-10-04T13:36:56Z

←Older revision		Revision as of 13:36, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='5uxm' size='340' side='right'caption='[[5uxm]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='5uxm' size='340' side='right'caption='[[5uxm]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uxm]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UXM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uxm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UXM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.54Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b7o\|2b7o]], [[5uxn\|5uxn]], [[5uxo\|5uxo]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2843 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxm OCA], [https://pdbe.org/5uxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uxm RCSB], [https://www.ebi.ac.uk/pdbsum/5uxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxm ProSAT]</span></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5uxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxm OCA], [~~http~~://pdbe.org/5uxm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5uxm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5uxm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxm ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9I000_PSEAE Q9I000_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Jameson~~, G B~~]]	+	[[Category: Jameson GB]]
-	[[Category: Parker~~, E J~~]]	+	[[Category: Parker EJ]]
-	[[Category: Sterritt~~, O W]]~~	+	[[Category: Sterritt OW]]
-	~~[[Category: 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase]]~~	+
-	~~[[Category: Dah7p]]~~	+
-	~~[[Category: Shikimate pathway]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 16:46, 20 November 2019

2019-11-20T16:46:04Z

←Older revision		Revision as of 16:46, 20 November 2019
Line 1:		Line 1:

	==Type II DAH7PS from Pseudomonas aeruginosa with Trp bound==		==Type II DAH7PS from Pseudomonas aeruginosa with Trp bound==
-	<StructureSection load='5uxm' size='340' side='right' caption='[[5uxm]], [[Resolution\|resolution]] 1.54Å' scene=''>	+	<StructureSection load='5uxm' size='340' side='right'caption='[[5uxm]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uxm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXM FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uxm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXM FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]		[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
		+	[[Category: Large Structures]]
	[[Category: Pseae]]		[[Category: Pseae]]
	[[Category: Jameson, G B]]		[[Category: Jameson, G B]]

OCA at 08:25, 2 May 2018

2018-05-02T08:25:38Z

←Older revision		Revision as of 08:25, 2 May 2018
Line 3:		Line 3:
	<StructureSection load='5uxm' size='340' side='right' caption='[[5uxm]], [[Resolution\|resolution]] 1.54Å' scene=''>		<StructureSection load='5uxm' size='340' side='right' caption='[[5uxm]], [[Resolution\|resolution]] 1.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5uxm]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5uxm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b7o\|2b7o]], [[5uxn\|5uxn]], [[5uxo\|5uxo]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b7o\|2b7o]], [[5uxn\|5uxn]], [[5uxo\|5uxo]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2843 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxm OCA], [http://pdbe.org/5uxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxm RCSB], [http://www.ebi.ac.uk/pdbsum/5uxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxm OCA], [http://pdbe.org/5uxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxm RCSB], [http://www.ebi.ac.uk/pdbsum/5uxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxm ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The shikimate pathway is responsible for the biosynthesis of key aromatic metabolites in microorganisms and plants. The enzyme 3-deoxy-d- arabino-heptulosonate 7-phosphate synthase (DAH7PS) catalyzes the first step of the pathway and DAH7PSs are classified as either type I or type II. The DAH7PSs from Pseudomonas aeruginosa are of particular interest as open reading frames encoding four putative DAH7PS isoenzymes, two classified as type Ialpha and two classified as type II, have been identified. Here, the structure of a type II DAH7PS enzyme from P. aeruginosa (PAO1) has been determined at 1.54 A resolution, in complex with its allosteric inhibitor tryptophan. Structural differences in the extra-barrel elements, when compared to other type II DAH7PS enzymes, directly relate to the formation of a distinct quaternary conformation with consequences for allosteric function and the control of flux to branching pathways. In contrast to the well-characterized Mycobacterium tuberculosis type II DAH7PS, which binds multiple allosteric inhibitors, this PaeDAH7PS(PA2843) is observed to be modestly allosterically inhibited by a single aromatic amino acid, tryptophan. In addition, structures in complex with tyrosine or with no allosteric ligand bound were determined. These structures provide new insights into the linkages between the active and allosteric sites. With four putative DAH7PS enzymes, P. aeruginosa appears to have evolved control of shikimate pathway flux at the genetic level, rather than control by multiple allosteric effectors to a single type II DAH7PS, as in M. tuberculosis. Type II DAH7PSs, thus, appear to have a more varied evolutionary trajectory than previously indicated.
		+
		+	A Pseudoisostructural Type II DAH7PS Enzyme from Pseudomonas aeruginosa: Alternative Evolutionary Strategies to Control Shikimate Pathway Flux.,Sterritt OW, Kessans SA, Jameson GB, Parker EJ Biochemistry. 2018 Apr 16. doi: 10.1021/acs.biochem.8b00082. PMID:29608284<ref>PMID:29608284</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5uxm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]		[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
		+	[[Category: Pseae]]
	[[Category: Jameson, G B]]		[[Category: Jameson, G B]]
	[[Category: Parker, E J]]		[[Category: Parker, E J]]

OCA at 06:24, 28 February 2018

2018-02-28T06:24:27Z

←Older revision		Revision as of 06:24, 28 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5uxm is ~~ON HOLD until Paper Publication~~	+	==Type II DAH7PS from Pseudomonas aeruginosa with Trp bound==
-		+	<StructureSection load='5uxm' size='340' side='right' caption='[[5uxm]], [[Resolution\|resolution]] 1.54Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5uxm]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UXM FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=PEP:PHOSPHOENOLPYRUVATE'>PEP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2b7o\|2b7o]], [[5uxn\|5uxn]], [[5uxo\|5uxo]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-deoxy-7-phosphoheptulonate_synthase 3-deoxy-7-phosphoheptulonate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.54 2.5.1.54] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uxm OCA], [http://pdbe.org/5uxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uxm RCSB], [http://www.ebi.ac.uk/pdbsum/5uxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uxm ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: 3-deoxy-7-phosphoheptulonate synthase]]
		+	[[Category: Jameson, G B]]
		+	[[Category: Parker, E J]]
		+	[[Category: Sterritt, O W]]
		+	[[Category: 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase]]
		+	[[Category: Dah7p]]
		+	[[Category: Shikimate pathway]]
		+	[[Category: Transferase]]

OCA at 11:25, 5 April 2017

2017-04-05T11:25:19Z

←Older revision		Revision as of 11:25, 5 April 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5uxm is ON HOLD	+	The entry 5uxm is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5uxm" [edit=sysop:move=sysop]

2017-03-09T15:04:03Z

Protected "5uxm" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:04, 9 March 2017

OCA: New page: '''Unreleased structure''' The entry 5uxm is ON HOLD Authors: Description: Category: Unreleased Structures

2017-03-09T15:04:02Z

New page: '''Unreleased structure''' The entry 5uxm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5uxm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]