5vk3 - Revision history

OCA at 13:50, 4 October 2023

2023-10-04T13:50:21Z

←Older revision		Revision as of 13:50, 4 October 2023
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	<StructureSection load='5vk3' size='340' side='right'caption='[[5vk3]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='5vk3' size='340' side='right'caption='[[5vk3]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vk3]] is a 2 chain structure with sequence from [~~http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50] and [http~~://en.wikipedia.org/wiki/~~Chatd Chatd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VK3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VK3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vk3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VK3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VK3 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.114Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bjs\|5bjs]]~~, [[~~5tqr~~\|~~5tqr~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CTHT_0029920 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=209285 CBS 144.50])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vk3 OCA], [https://pdbe.org/5vk3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vk3 RCSB], [https://www.ebi.ac.uk/pdbsum/5vk3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vk3 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vk3 OCA], [~~http~~://pdbe.org/5vk3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vk3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vk3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vk3 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0S8H7_CHATD G0S8H7_CHATD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Polycomb\|Polycomb]]	+	*[[Polycomb complex proteins 3D structures\|Polycomb complex proteins 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cbs 144 50]]~~	+	[[Category: Chaetomium thermophilum]]
-	~~[[Category: Chatd~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bratkowski~~, M A~~]]	+	[[Category: Bratkowski MA]]
-	[[Category: Liu, X]]	+	[[Category: Liu X]]
-	~~[[Category: Transferase~~]]	+

OCA at 09:36, 1 January 2020

2020-01-01T09:36:18Z

←Older revision		Revision as of 09:36, 1 January 2020
Line 1:		Line 1:

	==Apo ctPRC2 with E840A and K852D mutations in Ezh2==		==Apo ctPRC2 with E840A and K852D mutations in Ezh2==
-	<StructureSection load='5vk3' size='340' side='right' caption='[[5vk3]], [[Resolution\|resolution]] 2.11Å' scene=''>	+	<StructureSection load='5vk3' size='340' side='right'caption='[[5vk3]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vk3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50] and [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VK3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VK3 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vk3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50] and [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VK3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VK3 FirstGlance]. <br>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5vk3" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5vk3" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Polycomb\|Polycomb]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	[[Category: Cbs 144 50]]		[[Category: Cbs 144 50]]
	[[Category: Chatd]]		[[Category: Chatd]]
		+	[[Category: Large Structures]]
	[[Category: Bratkowski, M A]]		[[Category: Bratkowski, M A]]
	[[Category: Liu, X]]		[[Category: Liu, X]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 12:55, 6 November 2017

2017-11-06T12:55:25Z

←Older revision		Revision as of 12:55, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5vk3' size='340' side='right' caption='[[5vk3]], [[Resolution\|resolution]] 2.11Å' scene=''>		<StructureSection load='5vk3' size='340' side='right' caption='[[5vk3]], [[Resolution\|resolution]] 2.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vk3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VK3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VK3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vk3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_144.50 Cbs 144.50] and [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VK3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VK3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bjs\|5bjs]], [[5tqr\|5tqr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bjs\|5bjs]], [[5tqr\|5tqr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTHT_0029920 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=209285 CBS 144.50])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vk3 OCA], [http://pdbe.org/5vk3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vk3 RCSB], [http://www.ebi.ac.uk/pdbsum/5vk3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vk3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vk3 OCA], [http://pdbe.org/5vk3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vk3 RCSB], [http://www.ebi.ac.uk/pdbsum/5vk3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vk3 ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cbs 144 50]]
		+	[[Category: Chatd]]
	[[Category: Bratkowski, M A]]		[[Category: Bratkowski, M A]]
	[[Category: Liu, X]]		[[Category: Liu, X]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 11:41, 3 August 2017

2017-08-03T11:41:54Z

←Older revision		Revision as of 11:41, 3 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5vk3 is ~~ON HOLD~~	+	==Apo ctPRC2 with E840A and K852D mutations in Ezh2==
		+	<StructureSection load='5vk3' size='340' side='right' caption='[[5vk3]], [[Resolution\|resolution]] 2.11Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5vk3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VK3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VK3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5bjs\|5bjs]], [[5tqr\|5tqr]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vk3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vk3 OCA], [http://pdbe.org/5vk3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vk3 RCSB], [http://www.ebi.ac.uk/pdbsum/5vk3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vk3 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polycomb-group proteins control many fundamental biological processes, such as anatomical development in mammals and vernalization in plants. Polycomb repressive complex 2 (PRC2) is responsible for methylation of histone H3 lysine 27 (H3K27), and trimethylated H3K27 (H3K27me3) is implicated in epigenetic gene silencing. Recent genomic, biochemical, and structural data indicate that PRC2 is broadly conserved from yeast to human in many aspects. Here, we determined the crystal structure of an apo PRC2 from the fungus Chaetomium thermophilum captured in a bona fide autoinhibited state, which represents a novel conformation of PRC2 associated with enzyme regulation in light of the basal and stimulated states that we reported previously. We found that binding by the cofactor SAM mitigates this autoinhibited structural state. Using steady-state enzyme kinetics, we also demonstrated that disrupting the autoinhibition results in a vastly activated enzyme complex. Autoinhibition provides a novel structural platform that may enable control of PRC2 activity in response to diverse transcriptional states and chromatin contexts and set a ground state to allow PRC2 activation by other cellular mechanisms as well.

-	~~Authors: Bratkowski~~, M~~.A.~~, ~~Liu~~, X.	+	Polycomb repressive complex 2 in an autoinhibited state.,Bratkowski M, Yang X, Liu X J Biol Chem. 2017 Jun 12. pii: jbc.M117.787572. doi: 10.1074/jbc.M117.787572. PMID:28607149<ref>PMID:28607149</ref>

-	~~Description: Apo ctPRC2 with E840A and K852D mutations in Ezh2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: Bratkowski, M.A]]	+	<div class="pdbe-citations 5vk3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bratkowski, M A]]
	[[Category: Liu, X]]		[[Category: Liu, X]]
		+	[[Category: Transferase]]

OCA at 11:07, 10 May 2017

2017-05-10T11:07:27Z

←Older revision		Revision as of 11:07, 10 May 2017
Line 5:		Line 5:
	Authors: Bratkowski, M.A., Liu, X.		Authors: Bratkowski, M.A., Liu, X.

-	Description: Apo ~~form of methyltransferase from yeast~~	+	Description: Apo ctPRC2 with E840A and K852D mutations in Ezh2
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Bratkowski, M.A]]		[[Category: Bratkowski, M.A]]
	[[Category: Liu, X]]		[[Category: Liu, X]]

OCA: Protected "5vk3" [edit=sysop:move=sysop]

2017-04-27T12:23:01Z

Protected "5vk3" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:23, 27 April 2017

OCA: New page: '''Unreleased structure''' The entry 5vk3 is ON HOLD Authors: Bratkowski, M.A., Liu, X. Description: Apo form of methyltransferase from yeast Category: Unreleased Structures [[Cate...

2017-04-27T12:23:00Z

New page: '''Unreleased structure''' The entry 5vk3 is ON HOLD Authors: Bratkowski, M.A., Liu, X. Description: Apo form of methyltransferase from yeast Category: Unreleased Structures [[Cate...

New page

'''Unreleased structure'''

The entry 5vk3 is ON HOLD

Authors: Bratkowski, M.A., Liu, X.

Description: Apo form of methyltransferase from yeast
[[Category: Unreleased Structures]]
[[Category: Bratkowski, M.A]]
[[Category: Liu, X]]