5vky - Revision history

OCA at 14:11, 13 March 2024

2024-03-13T14:11:01Z

←Older revision		Revision as of 14:11, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='5vky' size='340' side='right'caption='[[5vky]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5vky' size='340' side='right'caption='[[5vky]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vky]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VKY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vky]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_JAY291 Saccharomyces cerevisiae JAY291]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VKY FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TDA2~~, ~~C1Q_03053 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=574961 Baker's yeast])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vky OCA], [~~http~~://pdbe.org/5vky PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vky RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vky PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vky ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vky OCA], [https://pdbe.org/5vky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vky RCSB], [https://www.ebi.ac.uk/pdbsum/5vky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vky ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/TDA2_YEAS2 TDA2_YEAS2]
-	Clathrin- and actin-mediated endocytosis is essential in eukaryotic cells. In this study, we demonstrate that Tda2 is a novel protein of the endocytic machinery necessary for normal internalization of native cargo in yeast. Tda2 has not been classified in any protein family. Unexpectedly, solving the crystal structure of Tda2 revealed it belongs to the dynein light chain family. However, Tda2 works independently of the dynein motor complex and microtubules. Tda2 forms a tight complex with the polyproline motif-rich protein Aim21, which interacts physically with the SH3 domain of the Arp2/3 complex regulator Bbc1. The Tda2-Aim21 complex localizes to endocytic sites in a Bbc1- and filamentous actin-dependent manner. Importantly, the Tda2-Aim21 complex interacts directly with and facilitates the recruitment of actin-capping protein, revealing barbed-end filament capping at endocytic sites to be a regulated event. Thus, we have uncovered a new layer of regulation of the actin cytoskeleton by a member of a conserved protein family that has not been previously associated with a function in endocytosis.	+
-		+
-	~~Novel function of a dynein light chain in actin assembly during clathrin-mediated endocytosis.,Farrell KB, McDonald S, Lamb AK, Worcester C, Peersen OB, Di Pietro SM J Cell Biol. 2017 Aug 7;216(8)~~:~~2565-2580. doi: 10.1083~~/~~jcb.201604123. Epub 2017, Jul 13. PMID:28706108<ref>PMID:28706108<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5vky" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~McDonald, S M]]~~	+	[[Category: Saccharomyces cerevisiae JAY291]]
-	~~[[Category: Peersen, O B~~]]	+	[[Category: Di Pietro SM]]
-	[[Category: Pietro~~, S M.Di~~]]	+	[[Category: McDonald SM]]
-	[[Category: ~~Dynein light chain clathrin mediated endocytosis~~]]	+	[[Category: Peersen OB]]
-	[[Category: ~~Protein binding~~]]	+

OCA at 08:04, 27 November 2019

2019-11-27T08:04:28Z

←Older revision		Revision as of 08:04, 27 November 2019
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	==Yeast Tda2 (YER071C) - a dynein light chain family member that works independently of the dynein motor complex and microtubules.==		==Yeast Tda2 (YER071C) - a dynein light chain family member that works independently of the dynein motor complex and microtubules.==
-	<StructureSection load='5vky' size='340' side='right' caption='[[5vky]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5vky' size='340' side='right'caption='[[5vky]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vky]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VKY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vky]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VKY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vky OCA], [http://pdbe.org/5vky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vky RCSB], [http://www.ebi.ac.uk/pdbsum/5vky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vky ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TDA2, C1Q_03053 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=574961 Baker's yeast])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vky OCA], [http://pdbe.org/5vky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vky RCSB], [http://www.ebi.ac.uk/pdbsum/5vky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vky ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: McDonald, S M]]		[[Category: McDonald, S M]]
	[[Category: Peersen, O B]]		[[Category: Peersen, O B]]

OCA at 06:03, 17 August 2017

2017-08-17T06:03:00Z

←Older revision		Revision as of 06:03, 17 August 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5vky is ~~ON HOLD~~	+	==Yeast Tda2 (YER071C) - a dynein light chain family member that works independently of the dynein motor complex and microtubules.==
		+	<StructureSection load='5vky' size='340' side='right' caption='[[5vky]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5vky]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VKY FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vky OCA], [http://pdbe.org/5vky PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vky RCSB], [http://www.ebi.ac.uk/pdbsum/5vky PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vky ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clathrin- and actin-mediated endocytosis is essential in eukaryotic cells. In this study, we demonstrate that Tda2 is a novel protein of the endocytic machinery necessary for normal internalization of native cargo in yeast. Tda2 has not been classified in any protein family. Unexpectedly, solving the crystal structure of Tda2 revealed it belongs to the dynein light chain family. However, Tda2 works independently of the dynein motor complex and microtubules. Tda2 forms a tight complex with the polyproline motif-rich protein Aim21, which interacts physically with the SH3 domain of the Arp2/3 complex regulator Bbc1. The Tda2-Aim21 complex localizes to endocytic sites in a Bbc1- and filamentous actin-dependent manner. Importantly, the Tda2-Aim21 complex interacts directly with and facilitates the recruitment of actin-capping protein, revealing barbed-end filament capping at endocytic sites to be a regulated event. Thus, we have uncovered a new layer of regulation of the actin cytoskeleton by a member of a conserved protein family that has not been previously associated with a function in endocytosis.

-	~~Authors: McDonald, S.M~~., ~~Di Pietro~~, S~~.M.~~, Peersen, O.B.	+	Novel function of a dynein light chain in actin assembly during clathrin-mediated endocytosis.,Farrell KB, McDonald S, Lamb AK, Worcester C, Peersen OB, Di Pietro SM J Cell Biol. 2017 Aug 7;216(8):2565-2580. doi: 10.1083/jcb.201604123. Epub 2017, Jul 13. PMID:28706108<ref>PMID:28706108</ref>

-	~~Description: Yeast Tda2 (YER071C) -~~a ~~dynein light chain family member that works independently~~ of the ~~dynein motor complex and microtubules~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	[[Category: ~~Mcdonald~~, S.M]]	+	<div class="pdbe-citations 5vky" style="background-color:#fffaf0;"></div>
-	[[Category: Peersen, O.B]]	+	== References ==
-	[[Category: Di Pietro, S.M]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: McDonald, S M]]
		+	[[Category: Peersen, O B]]
		+	[[Category: Pietro, S M.Di]]
		+	[[Category: Dynein light chain clathrin mediated endocytosis]]
		+	[[Category: Protein binding]]

OCA: Protected "5vky" [edit=sysop:move=sysop]

2017-05-04T11:25:25Z

Protected "5vky" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:25, 4 May 2017

OCA: New page: '''Unreleased structure''' The entry 5vky is ON HOLD Authors: McDonald, S.M., Di Pietro, S.M., Peersen, O.B. Description: Yeast Tda2 (YER071C) -a dynein light chain family member that ...

2017-05-04T11:25:24Z

New page: '''Unreleased structure''' The entry 5vky is ON HOLD Authors: McDonald, S.M., Di Pietro, S.M., Peersen, O.B. Description: Yeast Tda2 (YER071C) -a dynein light chain family member that ...

New page

'''Unreleased structure'''

The entry 5vky is ON HOLD

Authors: McDonald, S.M., Di Pietro, S.M., Peersen, O.B.

Description: Yeast Tda2 (YER071C) -a dynein light chain family member that works independently of the dynein motor complex and microtubules.
[[Category: Unreleased Structures]]
[[Category: Mcdonald, S.M]]
[[Category: Peersen, O.B]]
[[Category: Di Pietro, S.M]]