5vlj - Revision history

OCA at 22:10, 10 April 2020

2020-04-10T22:10:20Z

←Older revision		Revision as of 22:10, 10 April 2020
Line 3:		Line 3:
	<SX load='5vlj' size='340' side='right' viewer='molstar' caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>		<SX load='5vlj' size='340' side='right' viewer='molstar' caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vh9\|5vh9]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vh9\|5vh9]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DYN1, DHC1, YKR054C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PAC1, LIS1, SCY_5321 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DYN1, DHC1, YKR054C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PAC1, LIS1, SCY_5321 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlj OCA], [http://pdbe.org/5vlj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlj RCSB], [http://www.ebi.ac.uk/pdbsum/5vlj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5vlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlj OCA], [http://pdbe.org/5vlj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlj RCSB], [http://www.ebi.ac.uk/pdbsum/5vlj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 19:52, 6 March 2020

2020-03-06T19:52:26Z

←Older revision		Revision as of 19:52, 6 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of yeast cytoplasmic dynein with Walker B mutation at AAA3 in presence of ATP-VO4==		==Cryo-EM structure of yeast cytoplasmic dynein with Walker B mutation at AAA3 in presence of ATP-VO4==
-	<~~StructureSection~~ load='5vlj' size='340' side='right'caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>	+	<SX load='5vlj' size='340' side='right' viewer='molstar' caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>
Line 26:		Line 26:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 09:37, 1 January 2020

2020-01-01T09:37:03Z

←Older revision		Revision as of 09:37, 1 January 2020
Line 1:		Line 1:

	==Cryo-EM structure of yeast cytoplasmic dynein with Walker B mutation at AAA3 in presence of ATP-VO4==		==Cryo-EM structure of yeast cytoplasmic dynein with Walker B mutation at AAA3 in presence of ATP-VO4==
-	<StructureSection load='5vlj' size='340' side='right' caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>	+	<StructureSection load='5vlj' size='340' side='right'caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5vlj" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5vlj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Dynein 3D structures\|Dynein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Cianfrocco, M A]]		[[Category: Cianfrocco, M A]]
	[[Category: DeSantis, M E]]		[[Category: DeSantis, M E]]

OCA at 14:41, 6 November 2017

2017-11-06T14:41:47Z

←Older revision		Revision as of 14:41, 6 November 2017
Line 3:		Line 3:
	<StructureSection load='5vlj' size='340' side='right' caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>		<StructureSection load='5vlj' size='340' side='right' caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vh9\|5vh9]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vh9\|5vh9]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DYN1, DHC1, YKR054C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), PAC1, LIS1, SCY_5321 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlj OCA], [http://pdbe.org/5vlj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlj RCSB], [http://www.ebi.ac.uk/pdbsum/5vlj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlj OCA], [http://pdbe.org/5vlj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlj RCSB], [http://www.ebi.ac.uk/pdbsum/5vlj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlj ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 18824]]
	[[Category: Cianfrocco, M A]]		[[Category: Cianfrocco, M A]]
	[[Category: DeSantis, M E]]		[[Category: DeSantis, M E]]

OCA at 04:40, 21 September 2017

2017-09-21T04:40:21Z

←Older revision		Revision as of 04:40, 21 September 2017
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST]] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref> [[http://www.uniprot.org/uniprot/LIS1_YEAS7 LIS1_YEAS7]] Positively regulates the activity of the minus-end directed microtubule motor protein dynein. Plays a central role in positioning the mitotic spindle at the bud neck during cell division. Targets cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-mediated microtubule sliding along the bud cortex and consequently the movement of the mitotic spindle to the bud neck.		[[http://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST]] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref> [[http://www.uniprot.org/uniprot/LIS1_YEAS7 LIS1_YEAS7]] Positively regulates the activity of the minus-end directed microtubule motor protein dynein. Plays a central role in positioning the mitotic spindle at the bud neck during cell division. Targets cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-mediated microtubule sliding along the bud cortex and consequently the movement of the mitotic spindle to the bud neck.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Regulation is central to the functional versatility of cytoplasmic dynein, a motor involved in intracellular transport, cell division, and neurodevelopment. Previous work established that Lis1, a conserved regulator of dynein, binds to its motor domain and induces a tight microtubule-binding state in dynein. The work we present here-a combination of biochemistry, single-molecule assays, and cryoelectron microscopy-led to the surprising discovery that Lis1 has two opposing modes of regulating dynein, being capable of inducing both low and high affinity for the microtubule. We show that these opposing modes depend on the stoichiometry of Lis1 binding to dynein and that this stoichiometry is regulated by the nucleotide state of dynein's AAA3 domain. The low-affinity state requires Lis1 to also bind to dynein at a novel conserved site, mutation of which disrupts Lis1's function in vivo. We propose a new model for the regulation of dynein by Lis1.
		+
		+	Lis1 Has Two Opposing Modes of Regulating Cytoplasmic Dynein.,DeSantis ME, Cianfrocco MA, Htet ZM, Tran PT, Reck-Peterson SL, Leschziner AE Cell. 2017 Sep 7;170(6):1197-1208.e12. doi: 10.1016/j.cell.2017.08.037. PMID:28886386<ref>PMID:28886386</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5vlj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:51, 13 September 2017

2017-09-13T09:51:54Z

←Older revision		Revision as of 09:51, 13 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5vlj is ~~ON HOLD~~	+	==Cryo-EM structure of yeast cytoplasmic dynein with Walker B mutation at AAA3 in presence of ATP-VO4==
-		+	<StructureSection load='5vlj' size='340' side='right' caption='[[5vlj]], [[Resolution\|resolution]] 10.50Å' scene=''>
-	~~Authors~~: ~~Cianfrocco, M~~.A.~~, DeSantis, M~~.E., ~~Htet, Z~~.M., ~~Tran~~, P.T., ~~Reck-Peterson, S~~.L., ~~Leschziner, A~~.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5vlj]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VLJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VLJ FirstGlance]. <br>
-	~~Description~~: ~~Cryo~~-~~EM structure~~ of yeast ~~cytoplasmic~~ dynein ~~with Walker B mutation at AAA3~~ in ~~presence of ATP~~-~~VO4~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vh9\|5vh9]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vlj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vlj OCA], [http://pdbe.org/5vlj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vlj RCSB], [http://www.ebi.ac.uk/pdbsum/5vlj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vlj ProSAT]</span></td></tr>
-	[[Category: Leschziner, A.E]]	+	</table>
-	[[Category: Reck-Peterson, S.L]]	+	== Function ==
-	[[Category: ~~Htet~~, ~~Z.M~~]]	+	[[http://www.uniprot.org/uniprot/DYHC_YEAST DYHC_YEAST]] Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.<ref>PMID:15642746</ref> [[http://www.uniprot.org/uniprot/LIS1_YEAS7 LIS1_YEAS7]] Positively regulates the activity of the minus-end directed microtubule motor protein dynein. Plays a central role in positioning the mitotic spindle at the bud neck during cell division. Targets cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-mediated microtubule sliding along the bud cortex and consequently the movement of the mitotic spindle to the bud neck.
-	[[Category: ~~Tran, P.T~~]]	+	== References ==
-	[[Category: ~~Cianfrocco, M.A~~]]	+	<references/>
-	[[Category: ~~Desantis, M.E~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Cianfrocco, M A]]
		+	[[Category: DeSantis, M E]]
		+	[[Category: Htet, Z M]]
		+	[[Category: Leschziner, A E]]
		+	[[Category: Reck-Peterson, S L]]
		+	[[Category: Tran, P T]]
		+	[[Category: Cytoplasmic dynein]]
		+	[[Category: Lis1]]
		+	[[Category: Motor protein]]

OCA: Protected "5vlj" [edit=sysop:move=sysop]

2017-05-04T11:26:56Z

Protected "5vlj" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:26, 4 May 2017

OCA: New page: '''Unreleased structure''' The entry 5vlj is ON HOLD Authors: Cianfrocco, M.A., DeSantis, M.E., Htet, Z.M., Tran, P.T., Reck-Peterson, S.L., Leschziner, A.E. Description: Cryo-EM struc...

2017-05-04T11:26:55Z

New page: '''Unreleased structure''' The entry 5vlj is ON HOLD Authors: Cianfrocco, M.A., DeSantis, M.E., Htet, Z.M., Tran, P.T., Reck-Peterson, S.L., Leschziner, A.E. Description: Cryo-EM struc...

New page

'''Unreleased structure'''

The entry 5vlj is ON HOLD

Authors: Cianfrocco, M.A., DeSantis, M.E., Htet, Z.M., Tran, P.T., Reck-Peterson, S.L., Leschziner, A.E.

Description: Cryo-EM structure of yeast cytoplasmic dynein with Walker B mutation at AAA3 in presence of ATP-VO4
[[Category: Unreleased Structures]]
[[Category: Leschziner, A.E]]
[[Category: Reck-Peterson, S.L]]
[[Category: Htet, Z.M]]
[[Category: Tran, P.T]]
[[Category: Cianfrocco, M.A]]
[[Category: Desantis, M.E]]