5vtx - Revision history

OCA at 13:58, 4 October 2023

2023-10-04T13:58:27Z

←Older revision		Revision as of 13:58, 4 October 2023
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	<StructureSection load='5vtx' size='340' side='right'caption='[[5vtx]], [[Resolution\|resolution]] 2.65Å' scene=''>		<StructureSection load='5vtx' size='340' side='right'caption='[[5vtx]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vtx]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~I68a4 I68a4~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VTX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VTX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vtx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Hong_Kong/1/1968(H3N2)) Influenza A virus (A/Hong Kong/1/1968(H3N2))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VTX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vtq\|5vtq]], [[5vtr\|5vtr]], [[5vtu\|5vtu]], [[5vtv\|5vtv]], [[5vtw\|5vtw]], [[5vty\|5vty]], [[5vtz\|5vtz]], [[5vu4\|5vu4]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=506350 I68A4])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vtx OCA], [https://pdbe.org/5vtx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vtx RCSB], [https://www.ebi.ac.uk/pdbsum/5vtx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vtx ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vtx OCA], [~~http~~://pdbe.org/5vtx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vtx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vtx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vtx ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HEMA_I68A4 HEMA_I68A4]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).	+	[https://www.uniprot.org/uniprot/HEMA_I68A4 HEMA_I68A4] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: I68a4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Wilson~~, I A~~]]	+	[[Category: Wilson IA]]
-	[[Category: Wu~~, N C]]~~	+	[[Category: Wu NC]]
-	~~[[Category: Hemagglutinin]]~~	+
-	~~[[Category: Influenza a virus]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Receptor binding]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 17:24, 11 December 2019

2019-12-11T17:24:05Z

←Older revision		Revision as of 17:24, 11 December 2019
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	==Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225M/L226T/S228A mutant apo form==		==Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225M/L226T/S228A mutant apo form==
-	<StructureSection load='5vtx' size='340' side='right' caption='[[5vtx]], [[Resolution\|resolution]] 2.65Å' scene=''>	+	<StructureSection load='5vtx' size='340' side='right'caption='[[5vtx]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5vtx]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VTX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5vtx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/I68a4 I68a4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VTX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vtq\|5vtq]], [[5vtr\|5vtr]], [[5vtu\|5vtu]], [[5vtv\|5vtv]], [[5vtw\|5vtw]], [[5vty\|5vty]], [[5vtz\|5vtz]], [[5vu4\|5vu4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vtq\|5vtq]], [[5vtr\|5vtr]], [[5vtu\|5vtu]], [[5vtv\|5vtv]], [[5vtw\|5vtw]], [[5vty\|5vty]], [[5vtz\|5vtz]], [[5vu4\|5vu4]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=506350 I68A4])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vtx OCA], [http://pdbe.org/5vtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vtx RCSB], [http://www.ebi.ac.uk/pdbsum/5vtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vtx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vtx OCA], [http://pdbe.org/5vtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vtx RCSB], [http://www.ebi.ac.uk/pdbsum/5vtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vtx ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 5vtx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5vtx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: I68a4]]
		+	[[Category: Large Structures]]
	[[Category: Wilson, I A]]		[[Category: Wilson, I A]]
	[[Category: Wu, N C]]		[[Category: Wu, N C]]

OCA at 04:26, 21 September 2017

2017-09-21T04:26:27Z

←Older revision		Revision as of 04:26, 21 September 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5vtx is ~~ON HOLD~~	+	==Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225M/L226T/S228A mutant apo form==
		+	<StructureSection load='5vtx' size='340' side='right' caption='[[5vtx]], [[Resolution\|resolution]] 2.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5vtx]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VTX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vtq\|5vtq]], [[5vtr\|5vtr]], [[5vtu\|5vtu]], [[5vtv\|5vtv]], [[5vtw\|5vtw]], [[5vty\|5vty]], [[5vtz\|5vtz]], [[5vu4\|5vu4]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vtx OCA], [http://pdbe.org/5vtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vtx RCSB], [http://www.ebi.ac.uk/pdbsum/5vtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vtx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HEMA_I68A4 HEMA_I68A4]] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Influenza A virus hemagglutinin (HA) initiates viral entry by engaging host receptor sialylated glycans via its receptor-binding site (RBS). The amino acid sequence of the RBS naturally varies across avian and human influenza virus subtypes and is also evolvable. However, functional sequence diversity in the RBS has not been fully explored. Here, we performed a large-scale mutational analysis of the RBS of A/WSN/33 (H1N1) and A/Hong Kong/1/1968 (H3N2) HAs. Many replication-competent mutants not yet observed in nature were identified, including some that could escape from an RBS-targeted broadly neutralizing antibody. This functional sequence diversity is made possible by pervasive epistasis in the RBS 220-loop and can be buffered by avidity in viral receptor binding. Overall, our study reveals that the HA RBS can accommodate a much greater range of sequence diversity than previously thought, which has significant implications for the complex evolutionary interrelationships between receptor specificity and immune escape.

-	~~Authors: Nicholas C~~.Wu, ~~Ian A.~~Wilson	+	Diversity of Functionally Permissive Sequences in the Receptor-Binding Site of Influenza Hemagglutinin.,Wu NC, Xie J, Zheng T, Nycholat CM, Grande G, Paulson JC, Lerner RA, Wilson IA Cell Host Microbe. 2017 Jun 14;21(6):742-753.e8. doi: 10.1016/j.chom.2017.05.011. PMID:28618270<ref>PMID:28618270</ref>

-	~~Description: Crystal structure~~ of the A/~~Hong Kong~~/1/~~1968 (H3N2) influenza virus hemagglutinin G225M/L226T~~/~~S228A mutant apo form~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Nicholas C.~~Wu, ~~Ian A.Wilson~~]]	+	<div class="pdbe-citations 5vtx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Wilson, I A]]
		+	[[Category: Wu, N C]]
		+	[[Category: Hemagglutinin]]
		+	[[Category: Influenza a virus]]
		+	[[Category: Mutant]]
		+	[[Category: Receptor binding]]
		+	[[Category: Viral protein]]

OCA: Protected "5vtx" [edit=sysop:move=sysop]

2017-05-24T11:50:00Z

Protected "5vtx" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:50, 24 May 2017

OCA: New page: '''Unreleased structure''' The entry 5vtx is ON HOLD Authors: Nicholas C.Wu, Ian A.Wilson Description: Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin ...

2017-05-24T11:49:59Z

New page: '''Unreleased structure''' The entry 5vtx is ON HOLD Authors: Nicholas C.Wu, Ian A.Wilson Description: Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin ...

New page

'''Unreleased structure'''

The entry 5vtx is ON HOLD

Authors: Nicholas C.Wu, Ian A.Wilson

Description: Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin G225M/L226T/S228A mutant apo form
[[Category: Unreleased Structures]]
[[Category: Nicholas C.Wu, Ian A.Wilson]]