5w3x - Revision history

OCA at 14:06, 4 October 2023

2023-10-04T14:06:29Z

←Older revision		Revision as of 14:06, 4 October 2023
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	<StructureSection load='5w3x' size='340' side='right'caption='[[5w3x]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5w3x' size='340' side='right'caption='[[5w3x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896~~] and [~~http~~://en.wikipedia.org/wiki/~~Arath Arath~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5W3X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W3X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w40\|5w40]], [[5w3y\|5w3y]], [[5w3t\|5w3t]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">popp, RUN1985_v1_1190012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 "Bacillus solanacearum" Smith 1896]), RRS1, RCH2, RRS1-R, WRKY52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3x OCA], [https://pdbe.org/5w3x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w3x RCSB], [https://www.ebi.ac.uk/pdbsum/5w3x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3x ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5w3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3x OCA], [~~http~~://pdbe.org/5w3x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5w3x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5w3x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3x ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~WR52W_ARATH WR52W_ARATH~~]] Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Acts also as a disease resistance protein involved in resistance to fungal and bacterial pathogens, including R.solanacearum, P.syringae pv. tomato and C.higginsianum. In presence of RPS4, elicites an EDS1-dependent hypersensitive response (PubMed:24146667).[UniProtKB:Q9FH83]<ref>PMID:19519800</ref> <ref>PMID:19826224</ref> <ref>PMID:24146667</ref>	+	[https://www.uniprot.org/uniprot/A0A0S4VB05_RALSL A0A0S4VB05_RALSL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus solanacearum smith 1896]]~~	+	[[Category: Arabidopsis thaliana]]
-	~~[[Category: Arath~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Gao, L~~]]	+	[[Category: Ralstonia solanacearum]]
-	[[Category: ~~Song, J~~]]	+	[[Category: Gao L]]
-	[[Category: ~~Zhang, Z M~~]]	+	[[Category: Song J]]
-	[[Category: ~~Ip6]]~~	+	[[Category: Zhang ZM]]
-	~~[[Category: Popp2]]~~	+
-	~~[[Category: Rrs-r]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Wrky]]~~	+
-	~~[[Category: Yopj effector~~]]	+

OCA at 05:58, 14 October 2020

2020-10-14T05:58:05Z

←Older revision		Revision as of 05:58, 14 October 2020
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	<StructureSection load='5w3x' size='340' side='right'caption='[[5w3x]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5w3x' size='340' side='right'caption='[[5w3x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896] and [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5W3X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896] and [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5W3X FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w40\|5w40]], [[5w3y\|5w3y]], [[5w3t\|5w3t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w40\|5w40]], [[5w3y\|5w3y]], [[5w3t\|5w3t]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">popp, RUN1985_v1_1190012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 "Bacillus solanacearum" Smith 1896]), RRS1, RCH2, RRS1-R, WRKY52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">popp, RUN1985_v1_1190012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 "Bacillus solanacearum" Smith 1896]), RRS1, RCH2, RRS1-R, WRKY52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5w3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3x OCA], [http://pdbe.org/5w3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3x RCSB], [http://www.ebi.ac.uk/pdbsum/5w3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3x ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5w3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3x OCA], [http://pdbe.org/5w3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3x RCSB], [http://www.ebi.ac.uk/pdbsum/5w3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3x ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:45, 1 January 2020

2020-01-01T09:45:56Z

←Older revision		Revision as of 09:45, 1 January 2020
Line 1:		Line 1:

	==Crystal structure of PopP2 in complex with IP6, AcCoA and the WRKY domain of RRS1-R .==		==Crystal structure of PopP2 in complex with IP6, AcCoA and the WRKY domain of RRS1-R .==
-	<StructureSection load='5w3x' size='340' side='right' caption='[[5w3x]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='5w3x' size='340' side='right'caption='[[5w3x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896] and [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3X FirstGlance]. <br>		<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896] and [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3X FirstGlance]. <br>
Line 26:		Line 26:
	[[Category: Bacillus solanacearum smith 1896]]		[[Category: Bacillus solanacearum smith 1896]]
	[[Category: Arath]]		[[Category: Arath]]
		+	[[Category: Large Structures]]
	[[Category: Gao, L]]		[[Category: Gao, L]]
	[[Category: Song, J]]		[[Category: Song, J]]

OCA at 10:32, 22 November 2017

2017-11-22T10:32:23Z

←Older revision		Revision as of 10:32, 22 November 2017
Line 3:		Line 3:
	<StructureSection load='5w3x' size='340' side='right' caption='[[5w3x]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='5w3x' size='340' side='right' caption='[[5w3x]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_solanacearum"_smith_1896 "bacillus solanacearum" smith 1896] and [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3X FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w40\|5w40]], [[5w3y\|5w3y]], [[5w3t\|5w3t]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5w40\|5w40]], [[5w3y\|5w3y]], [[5w3t\|5w3t]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">popp, RUN1985_v1_1190012 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=305 "Bacillus solanacearum" Smith 1896]), RRS1, RCH2, RRS1-R, WRKY52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3x OCA], [http://pdbe.org/5w3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3x RCSB], [http://www.ebi.ac.uk/pdbsum/5w3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3x OCA], [http://pdbe.org/5w3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3x RCSB], [http://www.ebi.ac.uk/pdbsum/5w3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3x ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus solanacearum smith 1896]]
		+	[[Category: Arath]]
	[[Category: Gao, L]]		[[Category: Gao, L]]
	[[Category: Song, J]]		[[Category: Song, J]]

OCA: Protected "5w3x" [edit=sysop:move=sysop]

2017-08-09T09:11:04Z

Protected "5w3x" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:11, 9 August 2017

OCA: New page: ==Crystal structure of PopP2 in complex with IP6, AcCoA and the WRKY domain of RRS1-R .== == Structural highlights ==
<table><tr><td colspan='2'>[[5w3x]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W3X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W3X FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IHP:INOSITOL+HEXAKISPHOSPHATE'>IHP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5w40|5w40]], [[5w3y|5w3y]], [[5w3t|5w3t]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w3x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w3x OCA], [http://pdbe.org/5w3x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w3x RCSB], [http://www.ebi.ac.uk/pdbsum/5w3x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w3x ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/WR52W_ARATH WR52W_ARATH]] Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Acts also as a disease resistance protein involved in resistance to fungal and bacterial pathogens, including R.solanacearum, P.syringae pv. tomato and C.higginsianum. In presence of RPS4, elicites an EDS1-dependent hypersensitive response (PubMed:24146667).[UniProtKB:Q9FH83]<ref>PMID:19519800</ref> <ref>PMID:19826224</ref> <ref>PMID:24146667</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Yersinia outer protein J (YopJ) family of bacterial effectors depends on a novel acetyltransferase domain to acetylate signalling proteins from plant and animal hosts. However, the underlying mechanism is unclear. Here, we report the crystal structures of PopP2, a YopJ effector produced by the plant pathogen Ralstonia solanacearum, in complex with inositol hexaphosphate (InsP6), acetyl-coenzyme A (AcCoA) and/or substrate Resistance to Ralstonia solanacearum 1 (RRS1-R)WRKY. PopP2 recognizes the WRKYGQK motif of RRS1-RWRKY to position a targeted lysine in the active site for acetylation. Importantly, the PopP2-RRS1-RWRKY association is allosterically regulated by InsP6 binding, suggesting a previously unidentified role of the eukaryote-specific cofactor in substrate interaction. Furthermore, we provide evidence for the reaction intermediate of PopP2-mediated acetylation, an acetyl-cysteine covalent adduct, lending direct support to the 'ping-pong'-like catalytic mechanism proposed for YopJ effectors. Our study provides critical mechanistic insights into the virulence activity of YopJ class of acetyltransferases.

Mechanism of host substrate acetylation by a YopJ family effector.,Zhang ZM, Ma KW, Gao L, Hu Z, Schwizer S, Ma W, Song J Nat Plants. 2017 Jul 24;3:17115. doi: 10.1038/nplants.2017.115. PMID:28737762<ref>PMID:28737762</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5w3x" style="background-color:#fffaf0;"></div>
== References ==
<references/>
TOC
</StructureSection>
[[Category: Gao, L]]
[[Category: Song, J]]
[[Category: Zhang, Z M]]
[[Category: Ip6]]
[[Category: Popp2]]
[[Category: Rrs-r]]
[[Category: Transcription]]
[[Category: Wrky]]
[[Category: Yopj effector]]