5w68 - Revision history

OCA at 14:14, 13 March 2024

2024-03-13T14:14:06Z

←Older revision		Revision as of 14:14, 13 March 2024
Line 3:		Line 3:
	<SX load='5w68' size='340' side='right' viewer='molstar' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='5w68' size='340' side='right' viewer='molstar' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Eco27 Eco27~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5W68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O127:H6_str._E2348/69 Escherichia coli O127:H6 str. E2348/69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W68 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~gspD~~, ~~E2348C_3249 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=574521 ECO27])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5w68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w68 OCA], [~~http~~://pdbe.org/5w68 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5w68 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5w68 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5w68 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w68 OCA], [https://pdbe.org/5w68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w68 RCSB], [https://www.ebi.ac.uk/pdbsum/5w68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w68 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/B7UI37_ECO27 B7UI37_ECO27]
-	The beta-barrel assembly machinery (BAM) complex is the core machinery for the assembly of beta-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis yet do not require assistance from the BAM complex raises the question of how these proteins assemble into bacterial outer membranes. Here we address this question through a structural analysis of the type 2 secretion system (T2SS) secretin from enteropathogenic Escherichia coli O127:~~H6 strain E2348~~/69. Long beta-strands assemble into a barrel extending 17 A through, and beyond the outer membrane, adding insight to how these extensive beta-strands are assembled into the E. coli outer membrane. The substrate docking chamber of this secretin is shown to be sufficient to accommodate the substrate mucinase, SteC.IMPORTANCE: In order to cause disease, bacterial pathogens inhibit immune responses and induce pathology that will favour their replication and dissemination. In Gram-negative bacteria, these key attributes of pathogenesis depend on structures assembled into or onto the outer membrane. One of these is the T2SS. The Vibrio-type T2SS mediates cholera toxin secretion in Vibrio cholerae and, in Escherichia coli O127:H6 strain E2348/~~69, the same machinery mediates secretion of the mucinases that enable the pathogen to penetrate intestinal mucus and thereby establish deadly infections~~.	+
-		+
-	~~The structure and membrane topography of the Vibrio-type secretin complex from the T2SS of enteropathogenic Escherichia coli (EPEC)~~.~~,Hay ID, Belousoff MJ, Dunstan RA, Bamert RS, Lithgow T J Bacteriol. 2017 Oct 30. pii: JB.00521-17. doi: 10.1128~~/~~JB.00521-17. PMID:29084860<ref>PMID:29084860</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5w68" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Eco27~~]]	+	[[Category: Escherichia coli O127:H6 str. E2348/69]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bamert, R]]	+	[[Category: Bamert R]]
-	[[Category: Belousoff~~, M J~~]]	+	[[Category: Belousoff MJ]]
-	[[Category: Dunstan, R]]	+	[[Category: Dunstan R]]
-	[[Category: Hay~~, I D~~]]	+	[[Category: Hay ID]]
-	[[Category: Lithgow, T]]	+	[[Category: Lithgow T]]
-	~~[[Category: Homo oligomer]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Outer membrane complex]]~~	+
-	~~[[Category: Type 2 secretin~~]]	+

OCA at 22:18, 10 April 2020

2020-04-10T22:18:34Z

←Older revision		Revision as of 22:18, 10 April 2020
Line 3:		Line 3:
	<SX load='5w68' size='340' side='right' viewer='molstar' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='5w68' size='340' side='right' viewer='molstar' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco27 Eco27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5W68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco27 Eco27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5W68 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gspD, E2348C_3249 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=574521 ECO27])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gspD, E2348C_3249 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=574521 ECO27])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5w68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w68 OCA], [http://pdbe.org/5w68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w68 RCSB], [http://www.ebi.ac.uk/pdbsum/5w68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w68 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5w68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w68 OCA], [http://pdbe.org/5w68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w68 RCSB], [http://www.ebi.ac.uk/pdbsum/5w68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w68 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:55, 6 March 2020

2020-03-06T19:55:56Z

←Older revision		Revision as of 19:55, 6 March 2020
Line 1:		Line 1:

	==Type II secretin from Enteropathogenic Escherichia coli - GspD==		==Type II secretin from Enteropathogenic Escherichia coli - GspD==
-	<~~StructureSection~~ load='5w68' size='340' side='right' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<SX load='5w68' size='340' side='right' viewer='molstar' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco27 Eco27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W68 FirstGlance]. <br>		<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco27 Eco27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W68 FirstGlance]. <br>
Line 19:		Line 19:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Eco27]]		[[Category: Eco27]]
		+	[[Category: Large Structures]]
	[[Category: Bamert, R]]		[[Category: Bamert, R]]
	[[Category: Belousoff, M J]]		[[Category: Belousoff, M J]]

OCA at 08:16, 17 January 2018

2018-01-17T08:16:16Z

←Older revision		Revision as of 08:16, 17 January 2018
Line 3:		Line 3:
	<StructureSection load='5w68' size='340' side='right' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='5w68' size='340' side='right' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Eco27 Eco27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W68 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w68 OCA], [http://pdbe.org/5w68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w68 RCSB], [http://www.ebi.ac.uk/pdbsum/5w68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w68 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gspD, E2348C_3249 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=574521 ECO27])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w68 OCA], [http://pdbe.org/5w68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w68 RCSB], [http://www.ebi.ac.uk/pdbsum/5w68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w68 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Eco27]]
	[[Category: Bamert, R]]		[[Category: Bamert, R]]
	[[Category: Belousoff, M J]]		[[Category: Belousoff, M J]]

OCA at 07:34, 15 November 2017

2017-11-15T07:34:56Z

←Older revision		Revision as of 07:34, 15 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5w68 is ~~ON HOLD until Paper~~ Publication	+	==Type II secretin from Enteropathogenic Escherichia coli - GspD==
		+	<StructureSection load='5w68' size='340' side='right' caption='[[5w68]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5w68]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W68 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w68 OCA], [http://pdbe.org/5w68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w68 RCSB], [http://www.ebi.ac.uk/pdbsum/5w68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w68 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The beta-barrel assembly machinery (BAM) complex is the core machinery for the assembly of beta-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis yet do not require assistance from the BAM complex raises the question of how these proteins assemble into bacterial outer membranes. Here we address this question through a structural analysis of the type 2 secretion system (T2SS) secretin from enteropathogenic Escherichia coli O127:H6 strain E2348/69. Long beta-strands assemble into a barrel extending 17 A through, and beyond the outer membrane, adding insight to how these extensive beta-strands are assembled into the E. coli outer membrane. The substrate docking chamber of this secretin is shown to be sufficient to accommodate the substrate mucinase, SteC.IMPORTANCE: In order to cause disease, bacterial pathogens inhibit immune responses and induce pathology that will favour their replication and dissemination. In Gram-negative bacteria, these key attributes of pathogenesis depend on structures assembled into or onto the outer membrane. One of these is the T2SS. The Vibrio-type T2SS mediates cholera toxin secretion in Vibrio cholerae and, in Escherichia coli O127:H6 strain E2348/69, the same machinery mediates secretion of the mucinases that enable the pathogen to penetrate intestinal mucus and thereby establish deadly infections.

-	~~Authors: Hay, I.D~~., Belousoff~~, M.J.~~, Dunstan~~, R.~~, Bamert~~, R.~~, Lithgow, T.	+	The structure and membrane topography of the Vibrio-type secretin complex from the T2SS of enteropathogenic Escherichia coli (EPEC).,Hay ID, Belousoff MJ, Dunstan RA, Bamert RS, Lithgow T J Bacteriol. 2017 Oct 30. pii: JB.00521-17. doi: 10.1128/JB.00521-17. PMID:29084860<ref>PMID:29084860</ref>

-	~~Description: Type II secretin from Enteropathogenic Escherichia coli -GspD~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Belousoff, M.J]]~~	+	<div class="pdbe-citations 5w68" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bamert, R]]		[[Category: Bamert, R]]
		+	[[Category: Belousoff, M J]]
	[[Category: Dunstan, R]]		[[Category: Dunstan, R]]
		+	[[Category: Hay, I D]]
	[[Category: Lithgow, T]]		[[Category: Lithgow, T]]
-	[[Category: ~~Hay, I.D~~]]	+	[[Category: Homo oligomer]]
		+	[[Category: Membrane protein]]
		+	[[Category: Outer membrane complex]]
		+	[[Category: Type 2 secretin]]

OCA: Protected "5w68" [edit=sysop:move=sysop]

2017-09-27T14:29:07Z

Protected "5w68" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:29, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5w68 is ON HOLD until Paper Publication Authors: Hay, I.D., Belousoff, M.J., Dunstan, R., Bamert, R., Lithgow, T. Description: Type II secretin fro...

2017-09-27T14:29:06Z

New page: '''Unreleased structure''' The entry 5w68 is ON HOLD until Paper Publication Authors: Hay, I.D., Belousoff, M.J., Dunstan, R., Bamert, R., Lithgow, T. Description: Type II secretin fro...

New page

'''Unreleased structure'''

The entry 5w68 is ON HOLD until Paper Publication

Authors: Hay, I.D., Belousoff, M.J., Dunstan, R., Bamert, R., Lithgow, T.

Description: Type II secretin from Enteropathogenic Escherichia coli -GspD
[[Category: Unreleased Structures]]
[[Category: Belousoff, M.J]]
[[Category: Bamert, R]]
[[Category: Dunstan, R]]
[[Category: Lithgow, T]]
[[Category: Hay, I.D]]